These results indicate that MilA indirect ELISA can be utilized for the detection ofM. bovis antibodies in milk.
📌 3 Key Takeaways
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Objective & Hypothesis:The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA withthe aim to detect M. bovis antibodies in milk.
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Key Findings & Data:An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogeniclipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies.
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Conclusion & Impact:These results indicate that MilA indirect ELISA can be utilized for the detection ofM. bovis antibodies in milk.
🔬Design:Research Article
📍Scope:291 samples
⚙️Variables:Domain Parameters
Abstract
The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA withthe aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogeniclipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested withconventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds inSouth Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive for M.bovis in PCR and 166 (57.0%) in bacterial culture. These results indicate that MilA indirect ELISA can be utilized for the detection ofM. bovis antibodies in milk.
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Cite This Article
HOARE, A., AL-FARHA, A. A., WAWEGAMA, N., FIRESTONE, S., HEMMATZADEH, F., MOFFAT, J., KOJOURI, G. A., AZARI, A. A., AMANOLLAHI, R., PETROVSKI, K. (2022). Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodies in bovine milk. Turkish Journal of Veterinary and Animal Sciences, Cilt: 44 - Sayı: 3, 752–755. https://ajindex.com/articles/614807
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