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Journals / Turkish Journal of Veterinary and Animal Sciences / 2021 / Cilt: 45 - Sayı: 1

Caprine MHC gene polymorphism and its association with endoparasitic infestation (Haemonchus contortus) in Indian goat breeds

Thirunavukkarasu SBALAMURUGAN
Pushpendra KUMAR
Kush SHRIVASTAVA
Chinmoy MISHRA
Om PRAKASH
Amit KUMAR
Anuj CHAUHAN
Nihar Ranjan SAHOO
Manjit PANIGRAHI
Bharat BHUSHAN
Arvind PRASAD
Ilayakumar KAVERIYAPPAN
Sharavanan VELUSAMY
Pages
93–100
Publication Date
2022-11-01
DOI
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⚡ TL;DR(Too Long; Didn't Read)

There was variation among genotypes in mean values of FEC and PCV for all loci; however, the effect of genotypes on indicator traits was found to be statistically nonsignificant (P ≤ 0.05).Key words: Endoparasite, goat, MHC gene, PCR-RFLP, polymorphism

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Objective & Hypothesis:Abstract: The present study examined the variability of MHC class II DRB exon 2 gene using restriction fragment length polymorphism analysis of PCR-amplified fragments PCR-(RFLP), and its association with Haemonchus contortus infestation in Salem Black and Tellicherry goat population.
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Key Findings & Data:Pooled fecal coproculture and larval identification showed predominant presence of haemonchus (L3) larva.
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Conclusion & Impact:There was variation among genotypes in mean values of FEC and PCV for all loci; however, the effect of genotypes on indicator traits was found to be statistically nonsignificant (P ≤ 0.05).Key words: Endoparasite, goat, MHC gene, PCR-RFLP, polymorphism
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Abstract: The present study examined the variability of MHC class II DRB exon 2 gene using restriction fragment length polymorphism analysis of PCR-amplified fragments PCR-(RFLP), and its association with Haemonchus contortus infestation in Salem Black and Tellicherry goat population. Animals were naturally exposed to mixed infestation of endoparasites, predominantly Haemonchus contortus. Pooled fecal coproculture and larval identification showed predominant presence of haemonchus (L3) larva. Fecal egg count (FEC) and packed cell volume (PCV) were used as indicator traits. All the three studied loci, TaqI, BsaI, and BsaHI, were polymorphic having two alleles and three genotypes. The loci showed low to moderate values of polymorphic information content in both breeds. The mean fecal egg count estimates were 477.12 ± 34.14 and 730.42 ± 41.19 eggs per gram of feces for Salem Black and Tellicherry goats, respectively. The mean PCV values were within the normal range in both breeds; however, they showed negative correlation with FEC values. There was variation among genotypes in mean values of FEC and PCV for all loci; however, the effect of genotypes on indicator traits was found to be statistically nonsignificant (P ≤ 0.05).Key words: Endoparasite, goat, MHC gene, PCR-RFLP, polymorphism

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Cite This Article

SBALAMURUGAN, T., KUMAR, P., SHRIVASTAVA, K., MISHRA, C., PRAKASH, O., KUMAR, A., CHAUHAN, A., SAHOO, N. R., PANIGRAHI, M., BHUSHAN, B., PRASAD, A., KAVERIYAPPAN, I., VELUSAMY, S. (2022). Caprine MHC gene polymorphism and its association with endoparasitic infestation (Haemonchus contortus) in Indian goat breeds. Turkish Journal of Veterinary and Animal Sciences, Cilt: 45 - Sayı: 1, 93–100. https://ajindex.com/articles/614637

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