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Journals / Turkish Journal of Veterinary and Animal Sciences / 2021 / Cilt: 45 - Sayı: 3

Detection of Rhodococcus equi by PCR from foals and determination of antimicrobial susceptibility

Şükrü KIRKAN
Elif Oya ORAL
Uğur PARIN
Hafize Tuğba YÜKSEL DOLGUN
Pages
396–403
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The development of fast, economical and highly specific diagnostic technique against the threat of R. equi is essensial for better understanding of gene expressions related to resistance and development of effective immunization.Key words: Rhodococcus equi, foal pneumonia, PCR, vapA, antibacterial resistance

📌 3 Key Takeaways

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Objective & Hypothesis:Abstract: The scope of this research was determination of Rhodococcus equi isolates and antimicrobial susceptibilities in nasal swab specimen taken from animals between 0 and 6 months of age period.
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Key Findings & Data:R. equi was identified from 10 isolates (11.1%) by automatic identification device.
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Conclusion & Impact:The development of fast, economical and highly specific diagnostic technique against the threat of R. equi is essensial for better understanding of gene expressions related to resistance and development of effective immunization.Key words: Rhodococcus equi, foal pneumonia, PCR, vapA, antibacterial resistance
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Abstract: The scope of this research was determination of Rhodococcus equi isolates and antimicrobial susceptibilities in nasal swab specimen taken from animals between 0 and 6 months of age period. R. equi was identified from 10 isolates (11.1%) by automatic identification device. The verification of isolates were conducted by 16S ribosomal RNA PCR. VapA gene presence was detected in two (20%) isolates and multidrug resistance was identified against 11 antimicrobials. The antimicrobial resistance to macrolides and rifampin is arising worldwide since the excessive usage of the chemotherapeutics in various breeding farms just by evaluating monitoring outcomes. These results exhibit the presence of resistant strains among equine populations. The development of fast, economical and highly specific diagnostic technique against the threat of R. equi is essensial for better understanding of gene expressions related to resistance and development of effective immunization.Key words: Rhodococcus equi, foal pneumonia, PCR, vapA, antibacterial resistance

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Cite This Article

KIRKAN, Ş., ORAL, E. O., PARIN, U., DOLGUN, H. T. Y. (2022). Detection of Rhodococcus equi by PCR from foals and determination of antimicrobial susceptibility. Turkish Journal of Veterinary and Animal Sciences, Cilt: 45 - Sayı: 3, 396–403. https://ajindex.com/articles/614561

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References (5)

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