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Journals / Turkish Journal of Obstetrics and Gynecology / 2018 / Cilt: 15 - Sayı: 1

Tumor necrosis factor alfa and interleukin 1 alfa induced phosphorylation and degradation of inhibitory kappa B alpha are regulated by estradiol in endometrial cells

Endometriyal hücrelerdeki inhibitör kappa B alfa’nın tümör nekroz faktörü ve interlökin 1 indüklenmiş fosforilasyonunu estradiol ile ayarlanır

Ümit Ali KAYIŞLI
Aydın ARICI
Sefa ARLIER
Pages
50–59
Publication Date
2022-11-04
DOI
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⚡ TL;DR(Too Long; Didn't Read)

We then studied in cultured endometrial cells to assess the effects of estradiol on IкB and NF-кB function.Results: Normal endometrium from mid-late proliferative phase revealed the strongest IкB immunoreactivity throughout the cycle (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: When bound to the inhibitory kappa B (IкB) protein, the transcription factor nuclear factor kappa B (NF-кB) remains inactively in thecytoplasm.
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Key Findings & Data:Activated NF-кB upregulates the gene expression of many chemokines including monocyte chemoattractant protein-1 and interleukin (IL)-8.
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Conclusion & Impact:We then studied in cultured endometrial cells to assess the effects of estradiol on IкB and NF-кB function.Results: Normal endometrium from mid-late proliferative phase revealed the strongest IкB immunoreactivity throughout the cycle (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: When bound to the inhibitory kappa B (IкB) protein, the transcription factor nuclear factor kappa B (NF-кB) remains inactively in thecytoplasm. Activated NF-кB upregulates the gene expression of many chemokines including monocyte chemoattractant protein-1 and interleukin (IL)-8. We hypothesized that estrogen may regulate IкB phosphorylation and degradation thus influencing NF-кB-dependent gene expression. Regulation ofchemokines by estrogen is different in uterine endometrial cells when compared to ectopic endometrial cells of endometriosis.Materials and Methods: We investigated the in vivo expression of IкB in normal endometrium and in eutopic and ectopic endometrium of women withendometriosis. We then studied in cultured endometrial cells to assess the effects of estradiol on IкB and NF-кB function.Results: Normal endometrium from mid-late proliferative phase revealed the strongest IкB immunoreactivity throughout the cycle (p

Özet

Amaç: İnhibitör kappa B (IкB) protein bağlandığı zaman, transkripsiyon faktörü nükleer faktör kappa B (NF-кB) sitoplazmada aktive olamaz. Aktif NF-кB, monosit kemoatraktan proteini-1 ve interlökin (IL)-8 dahil olmak üzere pek çok kemokinin gen salınımını artırır. Biz östrojenin IкB-α fosforilasyonunu ve yıkımını düzenleyerek NF-кB’ye bağımlı genlerin salgılanmasını etkileyebileceğini varsaydık. Östrojen tarafından kemokinlerin düzenlenmesi uterus içindeki endometriyal hücrelerde, endometrioziste görülen uterus dışındaki endometriyal hücreler karşılaştırıldığında farklıdır. Gereç ve Yöntemler: Normal endometriyum, endometrioziz de uterus içindeki ve dışındaki endometriyal hücrelerden in vivo IкB salgılanmasını araştırdık. Ayrıca estradiolün IкB ve NF-кB fonksiyonu üzerindeki etkisini değerlendirmek için endometriyal hücre kültüründe çalıştık. Bulgular: Normal endometriyum bütün menstrual siklus boyunca orta-geç proliferatif fazda güçlü IкB immünreaktivite gözlemlendi (p

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Cite This Article

KAYIŞLI, Ü. A., ARICI, A., ARLIER, S. (2022). Tumor necrosis factor alfa and interleukin 1 alfa induced phosphorylation and degradation of inhibitory kappa B alpha are regulated by estradiol in endometrial cells. Turkish Journal of Obstetrics and Gynecology, Cilt: 15 - Sayı: 1, 50–59. https://ajindex.com/articles/613019

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