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Journals / Turkish Journal of Obstetrics and Gynecology / 2019 / Cilt: 16 - Sayı: 1

Inflammation-mediated fetal injury by maternal granulocyte-colony stimulating factor and high-dose intraamniotic endotoxin in the caprine model

Maternal granülosit koloni stimülan faktör ve yüksek doz intra-amniyotik endotoksin ile enflamasyon aracılı fetal beyin hasarı keçi modeli

Mekin SEZİK
Afşin KÖKER
Duygu KAŞIKÇI
Özlem ÖZMEN
Mehmet HALIGÜR
Ahmet AYDOĞAN
Orhan ÖZATİK
Pages
41–49
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

G-CSF for 5 days followed by 20 mg of IA endotoxin is a feasible caprine model to exacerbate intrauterine inflammation.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To define a novel experimental model with maternal intravenous (i.v.) granulocyte-colony stimulating factor (G-CSF) followed by a single- and high-dose of 20 mg intra-amniotic (IA) endotoxin to induce fetal brain injury in the preterm fetal goat.Materials and Methods: Pregnant goats (n=4) were given 50 microg/day G-CSF into the maternal jugular vein through gestational days 110-115 (term, 150 days).
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Key Findings & Data:At gestational day 115, 20 mg of IA endotoxin was administered.
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Conclusion & Impact:G-CSF for 5 days followed by 20 mg of IA endotoxin is a feasible caprine model to exacerbate intrauterine inflammation.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: To define a novel experimental model with maternal intravenous (i.v.) granulocyte-colony stimulating factor (G-CSF) followed by a single- and high-dose of 20 mg intra-amniotic (IA) endotoxin to induce fetal brain injury in the preterm fetal goat.Materials and Methods: Pregnant goats (n=4) were given 50 microg/day G-CSF into the maternal jugular vein through gestational days 110-115 (term, 150 days). At gestational day 115, 20 mg of IA endotoxin was administered. Following preterm delivery at day 120 by cesarean section umbilical cord, fetal lung and brain tissues were harvested for histopathology, immunohistochemistry, and electron microscopy. Inflammatory markers were evaluated in the amniotic fluid and fetal plasma.Results: Necrotizing funisitis with abundant leukocyte infiltration and fetal brain injury was induced in all the fetuses in the experimental group.Conclusion: Maternal i.v. G-CSF for 5 days followed by 20 mg of IA endotoxin is a feasible caprine model to exacerbate intrauterine inflammation.

Özet

Amaç: Preterm gebe keçide fetal beyin hasarı oluşturmak için maternal intravenöz (i.v.) granülosit koloni stimulan faktör (G-CSF) sonrasında amniyon içine tek ve yüksek-doz 20 mg endotoksin verilmesini içeren yeni deneysel modelin tanımlanmasıdır.Gereç ve Yöntemler: Gebeliğin 110-115. günlerinde (term: 150 gün) keçilere (n=4) jugular ven yoluyla 50 mikrog/gün G-CSF verildi. Gebeliğin 115. gününde, 20 mg IA endotoksin uygulandı. Gebeliğin 120. gününde sezaryen ile preterm doğumu takiben umblikal kord, fetal akciğer ve beyin örnekleri histopatoloji, immünhistokimya ve elektron mikroskobu incelemeleri için elde edildi. Amniyon sıvısı ve fetal plazmada enflamatuvar belirteçler değerlendirildi.Bulgular: Deney grubundaki fetüslerin tümünde, belirgin lökosit infiltrasyonu ile şekillenen nekrotizan funisit ve fetal beyin hasarının geliştiği saptandı.Sonuç: Şiddetli intrauterin enflamasyon oluşturmak için 5 gün maternal i.v. G-CSF ve takiben 20 mg IA endotoksin uygulamalarını içeren keçi modelinin uygulanabilir olduğu sonucuna varıldı.

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Cite This Article

SEZİK, M., KÖKER, A., KAŞIKÇI, D., ÖZMEN, Ö., HALIGÜR, M., AYDOĞAN, A., ÖZATİK, O. (2022). Inflammation-mediated fetal injury by maternal granulocyte-colony stimulating factor and high-dose intraamniotic endotoxin in the caprine model. Turkish Journal of Obstetrics and Gynecology, Cilt: 16 - Sayı: 1, 41–49. https://ajindex.com/articles/612998

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