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Journals / Turkish Journal of Medical Sciences / 1999 / Cilt: 29 - Sayı: 3

Immunohistochemical localization of thymosin Beta4 in bovine tissues

Ourania E. TSITSILONIS
Stanka STOEVE
Mirna MIHELIC-RAPP
Rainer LEHMANN
Wolfgang VOELTER
Ayhan ULUBELEN
W. GIEBEL
Pages
237–247
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In the latter case a relatively strong reaction was strictly located in the sarcolemn region of the myofibrils.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The histochemical localization of thymosin b4 was investigated for the first time in several bovine tissues, using the indirect immunofluorescent technique and the newly applied alkaline phosphatase anti-alkaline phosphatase (APAAP) antibody method.
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Key Findings & Data:A polyclonal antiserum raised against the N-terminal fragment (1-14) of thymosin b4, showing less than 0.1% crossreactivity with the second b-thymosin in calf tissues, thymosin b9, was used.
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Conclusion & Impact:In the latter case a relatively strong reaction was strictly located in the sarcolemn region of the myofibrils.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The histochemical localization of thymosin b4 was investigated for the first time in several bovine tissues, using the indirect immunofluorescent technique and the newly applied alkaline phosphatase anti-alkaline phosphatase (APAAP) antibody method. A polyclonal antiserum raised against the N-terminal fragment (1-14) of thymosin b4, showing less than 0.1% crossreactivity with the second b-thymosin in calf tissues, thymosin b9, was used. Positive reaction for thymosin b4 was observed in the cytoplasm of all tissues studied, i.e., bovine spleen, thymus, lung, liver, kidney, lymph nodes and cardiac muscle. The highest staining intensity was found in the cytoplasmic area of spleen and thymus, whereas in sections of muscle the reaction was very weak. In the latter case a relatively strong reaction was strictly located in the sarcolemn region of the myofibrils.

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Cite This Article

TSITSILONIS, O. E., STOEVE, S., MIHELIC-RAPP, M., LEHMANN, R., VOELTER, W., ULUBELEN, A., GIEBEL, W. (2022). Immunohistochemical localization of thymosin Beta4 in bovine tissues. Turkish Journal of Medical Sciences, Cilt: 29 - Sayı: 3, 237–247. https://ajindex.com/articles/612463

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