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Journals / Turkish Journal of Medical Sciences / 2001 / Cilt: 31 - Sayı: 1

Sandwich-Type Enzyme Immunoassay for Amyloid A4 Protein in Cerebrospinal Fluid From Patients with Head Trauma

İbrahim PİRİM
Pages
47–50
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The assay was highly specific for A4, demonstrating no cross reactivity between polyclonal anti A4 and monoclonal A4 antibodies (

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value.
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Key Findings & Data:A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP).
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Conclusion & Impact:The assay was highly specific for A4, demonstrating no cross reactivity between polyclonal anti A4 and monoclonal A4 antibodies (
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Beta-Amyloid protein (A4) · Head Trauma · ELISA

Abstract

The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value. For this purpose, a sandwich type ELISA assay was used. A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP). The polyclonal rabbit antibody against synthetic A4 protein (1-40 residues) was used as an immobilized antibody. A mouse monoclonal antibody against synthetic A4 protein (1-28 residues) was used as a different immunoglobulin to attach the A4. Enzyme-labelled anti-immunoglobulin (peroxidase rabbit anti-mouse antibody, PRAM) was used for reaction with mouse immunoglobulin. The assay was highly specific for A4, demonstrating no cross reactivity between polyclonal anti A4 and monoclonal A4 antibodies (

Özet

The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value. For this purpose, a sandwich type ELISA assay was used. A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP). The polyclonal rabbit antibody against synthetic A4 protein (1-40 residues) was used as an immobilized antibody. A mouse monoclonal antibody against synthetic A4 protein (1-28 residues) was used as a different immunoglobulin to attach the A4. Enzyme-labelled anti-immunoglobulin (peroxidase rabbit anti-mouse antibody, PRAM) was used for reaction with mouse immunoglobulin. The assay was highly specific for A4, demonstrating no cross reactivity between polyclonal anti A4 and monoclonal A4 antibodies (

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Cite This Article

PİRİM, İ. (2022). Sandwich-Type Enzyme Immunoassay for Amyloid A4 Protein in Cerebrospinal Fluid From Patients with Head Trauma. Turkish Journal of Medical Sciences, Cilt: 31 - Sayı: 1, 47–50. https://ajindex.com/articles/612307

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