AI Summary & Key Takeaways
AI InsightThe MICs obtained with each of the methods were read after 24 h and 48 h of incubation.Results: Overall agreement rates within ±1 dilution between the 2 methods were 86.7% (24 h) and 94.1% (48 h) for amphotericin B, and 85.5% (24 h) and 73.3% (48 h) for fluconazole.
📌 3 Key Takeaways
Abstract
Aim: Antifungal susceptibility testing may be an important aid in the treatment of patients with life threatening yeast infections. The aim of this study was to determine the susceptibility of Candida isolates obtained from clinical specimens to the antifungal agents amphotericin B and fluconazole, which are frequently used in our clinical practice. Materials and methods: Susceptibility to antifungal agents was investigated using the Etest (AB Biodisk, Solna, Sweden) and the reference broth macrodilution susceptibility test (CLSI M27-A2). The MICs obtained with each of the methods were read after 24 h and 48 h of incubation.Results: Overall agreement rates within ±1 dilution between the 2 methods were 86.7% (24 h) and 94.1% (48 h) for amphotericin B, and 85.5% (24 h) and 73.3% (48 h) for fluconazole. All C. albicans, C. pelliculosa, C. glabrata, and C. kefyr isolates were inhibited by ≤1 μg mL−1 of amphotericin B, and fluconazole resistance was not observed in C. albicans or C. tropicalis isolates according to the 2 methods. Conclusion: According to the results of this study, decreased susceptibility to fluconazole and amphotericin B was most prominent in C. parapsilosis, C. krusei, and C. glabrata isolates.
Özet
Amaç: Antifungal duyarlılık testleri, yaşamı tehdit eden maya mantarı enfeksiyonu olan hastaların tedavisinde büyük önem taşımaktadır. Bu çalışmanın amacı klinik örneklerden soyutlanan Candida kökenlerinin pratik uygulamada sıklıkla kullanılan antifungal ilaçlar olan amfoterisin B ve flukonazole duyarlılıklarının belirlenmesidir.Yöntem ve gereç: Antifungal ilaçlara duyarlılık, Etest (AB Biodisk, Solna, İsveç) ve referans broth makrodilüsyon duyarlılık testi (CLSI M27-A2) ile araştırıldı. MİK değerleri, her iki yöntem ile 24 ve 48 saatlik inkübasyonun sonunda değerlendirildi. Bulgular: İki yöntem arasında (±) 1 dilüsyon içinde uyum oranları, amfoterisin B için % 86,7 (24 saat) ve % 94,1 (48 saat), flukonazol için % 85,5 (24 saat) ve % 73,3 (48 saat) idi. C. albicans, C. pelliculosa, C. glabrata ve C. kefyr kökenlerinin tümü, ≤1 μg mL−1 konsantrasyonda amfoterisin B ile inhibe oldu ve C. albicans ya da C. tropicalis kökenlerinde her iki yöntem ile flukonazole direnç saptanmadı.Sonuç: Bu çalışmada, flukonazol ve amfoterisin B’ye duyarlılığın azalması, en belirgin olarak C. parapsilosis, C. krusei ve C. glabrata kökenlerinde görüldü.