In addition, KGF treatment resulted in higherlevels of SP-C on days 2 and 8 while lowering them on day 4, higher levels of AQP5 on day 4 while lowering them on day 8, and higherlevels of TTF-1 on days 2, 6, and 8.Conclusion: KGF treatment promoted proliferation of AECIIs, inhibited cell apoptosis, promoted transdiferentiation of AECIIs, andinduced alveolar epithelial type I cells to revert to AECIIs.
📌 3 Key Takeaways
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Objective & Hypothesis:Background/aim: To investigate the efects of keratinocyte growth factor (KGF) on the growth and transdiferentiation of primaryalveolar epithelial type II cells (AECIIs).Materials and methods: Te number of primary AECIIs, their viability, and cell cycle and apoptosis were studied under KGF treatmentusing a hemocytometer, trypan blue exclusion, and fow cytometry, respectively.
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Key Findings & Data:Positive expressions of surfactant protein C (SP-C),aquaporin 5 (AQP5), and thyroid transcription factor 1(TTF-1) were examined with indirect immunofuorescence; mRNA levels of SP-C, AQP5 , and TTF-1 were determined by polymerase chain reaction.Results: In response to KGF treatment, cell numbers were signifcantly increased, more cells were blocked in the S phase, and fewercells were apoptotic or necrotic on days 2 and 4, but there was little efect on cell viability.
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Conclusion & Impact:In addition, KGF treatment resulted in higherlevels of SP-C on days 2 and 8 while lowering them on day 4, higher levels of AQP5 on day 4 while lowering them on day 8, and higherlevels of TTF-1 on days 2, 6, and 8.Conclusion: KGF treatment promoted proliferation of AECIIs, inhibited cell apoptosis, promoted transdiferentiation of AECIIs, andinduced alveolar epithelial type I cells to revert to AECIIs.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
Background/aim: To investigate the efects of keratinocyte growth factor (KGF) on the growth and transdiferentiation of primaryalveolar epithelial type II cells (AECIIs).Materials and methods: Te number of primary AECIIs, their viability, and cell cycle and apoptosis were studied under KGF treatmentusing a hemocytometer, trypan blue exclusion, and fow cytometry, respectively. Positive expressions of surfactant protein C (SP-C),aquaporin 5 (AQP5), and thyroid transcription factor 1(TTF-1) were examined with indirect immunofuorescence; mRNA levels of SP-C, AQP5 , and TTF-1 were determined by polymerase chain reaction.Results: In response to KGF treatment, cell numbers were signifcantly increased, more cells were blocked in the S phase, and fewercells were apoptotic or necrotic on days 2 and 4, but there was little efect on cell viability. In addition, KGF treatment resulted in higherlevels of SP-C on days 2 and 8 while lowering them on day 4, higher levels of AQP5 on day 4 while lowering them on day 8, and higherlevels of TTF-1 on days 2, 6, and 8.Conclusion: KGF treatment promoted proliferation of AECIIs, inhibited cell apoptosis, promoted transdiferentiation of AECIIs, andinduced alveolar epithelial type I cells to revert to AECIIs.
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Cite This Article
WU, Y., LIU, X., FENG, Z., TIAN, C., KONG, X., LV, C. (2022). Efect of keratinocyte growth factor on growth and transdiferentiation of primary alveolar epithelial type II cells. Turkish Journal of Medical Sciences, Cilt: 45 - Sayı: 2, 251–260. https://ajindex.com/articles/610949
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