SavedAdd your journal

Journals / Turkish Journal of Medical Sciences / 2015 / Cilt: 45 - Sayı: 1

Comparative study of virulence factors among ESβL-producing and nonproducing Pseudomonas aeruginosa clinical isolates

Fatma IBRAHIM SONBOL
Abdel Fattah Badr MOHAMED
Maha Abd El Fattah KHALIL
Sameh Samir ALI
Pages
60–69
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The results of this study suggest a correlation between ESβL phenotype and the production of some factors that are reported to be involved in the virulence of P. aeruginosa.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background/aim: β-Lactamase production is considered one of the most important resistance mechanisms among virulent Pseudomonas aeruginosa isolates.
📊
Key Findings & Data:Materials and methods: Out of 183 different clinical specimens, 104 Pseudomonas aeruginosa isolates were recovered.
💡
Conclusion & Impact:Conclusion: The results of this study suggest a correlation between ESβL phenotype and the production of some factors that are reported to be involved in the virulence of P. aeruginosa.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background/aim: β-Lactamase production is considered one of the most important resistance mechanisms among virulent Pseudomonas aeruginosa isolates. The aim of this study was to compare the production and antimicrobial resistance patterns of some virulence factors in extended spectrum β-lactamase (ESβL)-producing and nonproducing P. aeruginosa clinical isolates. Materials and methods: Out of 183 different clinical specimens, 104 Pseudomonas aeruginosa isolates were recovered. The isolates were screened for ESβL production using the double disk diffusion test and phenotypic confirmatory disk diffusion test. All isolates were tested for susceptibility to 25 antimicrobials, as well as for expression of various virulence factors including pigment, hemolysin, gelatinase, protease, lipase, rhamnolipids, biofilm, and cell surface hydrophobicity. The results of ESβL producers and nonproducers were statistically compared. Results: All isolates showed a high frequency of multiple resistance to at least 14 and up to 25 of the tested antimicrobials. Nevertheless, most virulence factors were produced at higher rates in ESβL-producing than in ESβL-nonproducing Pseudomonas aeruginosa isolates. Conclusion: The results of this study suggest a correlation between ESβL phenotype and the production of some factors that are reported to be involved in the virulence of P. aeruginosa.

🔖

Cite This Article

SONBOL, F. I., MOHAMED, A. F. B., KHALIL, M. A. E. F., ALI, S. S. (2022). Comparative study of virulence factors among ESβL-producing and nonproducing Pseudomonas aeruginosa clinical isolates. Turkish Journal of Medical Sciences, Cilt: 45 - Sayı: 1, 60–69. https://ajindex.com/articles/610946

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (16)

  1. Ullah A, Durrani R, Ullah I, Rafiq M. Antibiotic resistance profile of clinical gram negative bacteria. J Biologic Food Sci Res 2012; 1: 23–25
  2. Morales-Espinosa R, Soberon-Chavez G, Delgado-Sapien G, Sandner-Miranda L. Genetic and phenotypic characterization of a Pseudomonas aeruginosa population with high frequency of genomic islands. PLOS ONE 2012; 7: e37459
  3. Kindrachuk KN. Activity of and resistance to cationic antimicrobial agents in Pseudomonas aeruginosa. J Antimicrob Chemother 2010; 29: 427–433
  4. Timani RJ. Molecular typing and biofilm characterization of Pseudomonas aeruginosa isolated from clinical samples in Lebanon. MSc, Lebanese American University, Beirut, Lebanon, 2012
  5. Kapoor R, Wadman MW, Dohm MT, Czyzewski AM, Spormann AM, Barron AE. Antimicrobial peptoids are effective against Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother 2011; 55: 3054–3057
  6. Mansouri S, Norouzi F, Moradi M, Nakhaae N. Comparison of virulence factors among clinical isolates of Pseudomonas aeruginosa producing and non-producing extended spectrum beta-lactamases. Curr Res Bacteriol 2011; 4: 85–93
  7. Kalaivani R. Antibiotic resistance mechanisms in clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. MD thesis, Pondicherry Institute of Medical Sciences, Puducherry, India; 2011
  8. Taslima Y. Prevalence of ESβL among E. coli and Klebsiella Spp. in a tertiary care hospital and molecular detection of important ESβL producing genes by multiplex PCR. PhD, Mymensingh Medical College, Mymensingh, Bangladesh; 2012
  9. Krieg NR, Holt JG, editors. Bergey’s Manual for Systematic Bacteriology, Vol
  10. Baltimore, MD, USA: Williams and Wilkins; 2001
  11. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 20th Informational Supplement. Wayne, PA, USA: Clinical and Laboratory Standards Institute; 2010
  12. Brooks E, Melnick JL, Adelberg EA. Medical Microbiology. 24th ed. New York, NY, USA: McGraw-Hill; 2007
  13. Pavlov D, De Wet CME, Grabow WOK, Ehlers MM. Potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. Int J Food Microbiol 2004; 92: 275–287
  14. MacFaddin JF. Biochemical Tests for Identification of Medical Bacteria. 2nd ed. Baltimore, MD, USA: Williams and Wilkins; 1980
  15. Madigan MT, Martinko JM, Parker J. Brock Biology of Microorganisms. 9th ed. Upper Saddle River, NJ, USA: Prentice Hall; 1999
  16. Zulia