SavedAdd your journal

Journals / Turkish Journal of Medical Sciences / 2016 / Cilt: 46 - Sayı: 3

The effects of bupivacaine combined with different adjuvants on block onset and duration and on ion channel expressions (SCN9A, TRPM) in sciatic nerve block in rats

Davut Sinan KAPLAN
Levent ŞAHİN
Tuncer DEMİR
Hasan ŞİMŞEK
Mehrican ŞAHİN
Beyhan CENGİZ
Nurgül DOLU
Pages
926–935
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Because the SCN9A and TRPM2,4,7 expression ratios of the BE group showed the least amount of change, this group had the best cellular integrity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background/aim: The objective of this experimental study was to examine the effects of epinephrine, dexmedetomidine, and clonidine added as adjuvants to bupivacaine on block onset and effect times, as well as the effects on the Na+ and Ca+2 channel gene expressions, which may indicate cell damage in the sciatic nerve cell membrane.
📊
Key Findings & Data:For each group, 0.2 mL of local anesthetic was injected into the sciatic nerve bifurcation point of the right leg.
💡
Conclusion & Impact:Because the SCN9A and TRPM2,4,7 expression ratios of the BE group showed the least amount of change, this group had the best cellular integrity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background/aim: The objective of this experimental study was to examine the effects of epinephrine, dexmedetomidine, and clonidine added as adjuvants to bupivacaine on block onset and effect times, as well as the effects on the Na+ and Ca+2 channel gene expressions, which may indicate cell damage in the sciatic nerve cell membrane. Materials and methods: Rats were divided into five groups: Group S (sham), saline solution; Group B, bupivacaine; Group BD, bupivacaine + dexmedetomidine; Group BC, bupivacaine + clonidine; and Group BE, bupivacaine + epinephrine. For each group, 0.2 mL of local anesthetic was injected into the sciatic nerve bifurcation point of the right leg. Sensory (proprioceptive and nociceptive block) and motor block onset and ending times were recorded. Results: The shortest onset time for the examined sciatic block was observed in the BC group, whereas the longest sensory and motor block times were observed in the BD group. The present data suggest suppressed TRPM7 and increased TRPM2 in the groups other than the BE group. Conclusion: Clonidine is more suitable for fast onset of peripheral nerve blocks, whereas the addition of dexmedetomidine is better in terms of duration. Because the SCN9A and TRPM2,4,7 expression ratios of the BE group showed the least amount of change, this group had the best cellular integrity.

🔖

Cite This Article

KAPLAN, D. S., ŞAHİN, L., DEMİR, T., ŞİMŞEK, H., ŞAHİN, M., CENGİZ, B., DOLU, N. (2022). The effects of bupivacaine combined with different adjuvants on block onset and duration and on ion channel expressions (SCN9A, TRPM) in sciatic nerve block in rats. Turkish Journal of Medical Sciences, Cilt: 46 - Sayı: 3, 926–935. https://ajindex.com/articles/610879

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Cuvillon P NE, Ripart J, Boyer JC, Dehour L, Mahamat A, L’hermite J, Boisson C, Vialles N, Lefrant JY, de La Coussaye JE. Comparison of the pharmacodynamics and pharmacokinetics of bupivacaine, ropivacaine (with epinephrine) and their equal volume mixtures with lidocaine used for femoral and sciatic nerve blocks. Anesth Analg 2009; 108: 641-649
  2. Berridge MJ, Lipp P, Bootman MD. The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 2000; 1: 11-21
  3. Harteneck C, Plant TD, Schultz G. From worm to man: three subfamilies of TRP channels. Trends Neurosci 2000; 23: 159- 166
  4. Montell C. Physiology, phylogeny, and functions of the TRP superfamily of cation channels. Sci STKE 2001; 2001: re1
  5. Montell C, Birnbaumer L, Flockerzi V. The TRP channels, a remarkably functional family. Cell 2002; 108: 595-598
  6. Alexander SP, Mathie A, Peters JA. Guide to receptors and channels, 1st edition. Br J Pharmacol 2004; 141 Suppl 1: S1- 126
  7. RM. T. Transient receptor potential melastatin 6 and 7 channels, magnesium transport, and vascular biology: implications in hypertension. Am J Physiol 2008; 294: H1103-H1118
  8. Kraft R HC. The mammalian melastatin-related transient receptor potential cation channels: an overview. Pflugers Arch 2005; 451: 204-211
  9. Kunert-Keil C, Bispring F, Kruger J, Brinkmeier H. Tissuespecific expression of TRP channel genes in the mouse and its variation in three different mouse strains. BMC Genomics 2006; 7: 159-163
  10. Montell C. An exciting release on TRPM7. Neuron 2006; 52: 395-397
  11. Vennekens R VT, Bindels RJ, Droogmans G, Nilius B. Current understanding of mammalian TRP homologues. Cell Calcium 2002; 31: 253-264
  12. Hara Y WM, Ishii M, Maeno E, Nishida M, Yoshida T, Yamada H, Shimizu S, Mori E, Kudoh J, Shimizu N et al. LTRPC2 Ca2+ permeable channel activated by changes in redox status confers susceptibility to cell death. Mol Cell Biochem 2002; 9: 163-173
  13. Perraud C JA, Inabe CO, Smith K, Penner MK, Kurosaki R, Fleig Scharenberg T. Regulation of vertebrate cellular Mg+2 homeostasis by TRPM7. Cell 2003; 114: 191-200
  14. Perraud AL KH, Schmitz C. Novel aspects of signaling and ion homeostasis regulation in immunocytes. The TRPM ion channels and their potential role in modulating the immune response. Mol Immunol 2004; 41: 657-673
  15. Earley S, Waldron BJ, Brayden JE. Critical role for transient receptor potential channel TRPM4 in myogenic constriction of cerebral arteries. Circ Res 2004; 95: 922-92