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Journals / Turkish Journal of Medical Sciences / 2018 / Cilt: 48 - Sayı: 3

In vitro susceptibility of seven antifungal agents against dermatophytes isolated in İstanbul

Ayşe BARIŞ
Sümeyye ŞEN
Fatma ÖZAKKAŞ
Rabiye ALTINBAŞ
Deniz TURAN
Pages
615–619
Publication Date
2022-11-05
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Inthis study, we aimed to determine the effectiveness of seven antifungal agents: amphotericin B, terbinafine, itraconazole, voriconazole,ketoconazole, miconazole, and fluconazole.Materials and methods: A sensitivity study was performed using a microdilution method in accordance with the CLSI M38-A2standards using isolates of Trichophyton rubrum (n = 55), Microsporum canis (n = 9), and Trichophyton interdigitale (n = 2), which wereidentified by sequencing the internal transcribed spacer region of the rDNA.Results: According to the results of antifungal sensitivity tests, the geometric mean (GM) minimum inhibitory concentration (MIC)against T. rubrum was 0.10 μg/mL for ketoconazole, 0.20 μg/mL for itraconazole, 0.07 μg/mL for miconazole, 0.48 μg/mL for fluconazole,2.27 μg/mL for amphotericin B, 0.06 μg/mL for voriconazole, and 0.06 μg/mL for terbinafine.

📌 3 Key Takeaways

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Objective & Hypothesis:Background/aim: Dermatophytes are the causative agents of dermatophytosis, which is a common disease worldwide that affects thehair, skin, and nails.
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Key Findings & Data:Dermatophytes comprise more than 40 species in 3 genera: Microsporum, Trichophyton, and Epidermaphyton.
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Conclusion & Impact:Inthis study, we aimed to determine the effectiveness of seven antifungal agents: amphotericin B, terbinafine, itraconazole, voriconazole,ketoconazole, miconazole, and fluconazole.Materials and methods: A sensitivity study was performed using a microdilution method in accordance with the CLSI M38-A2standards using isolates of Trichophyton rubrum (n = 55), Microsporum canis (n = 9), and Trichophyton interdigitale (n = 2), which wereidentified by sequencing the internal transcribed spacer region of the rDNA.Results: According to the results of antifungal sensitivity tests, the geometric mean (GM) minimum inhibitory concentration (MIC)against T. rubrum was 0.10 μg/mL for ketoconazole, 0.20 μg/mL for itraconazole, 0.07 μg/mL for miconazole, 0.48 μg/mL for fluconazole,2.27 μg/mL for amphotericin B, 0.06 μg/mL for voriconazole, and 0.06 μg/mL for terbinafine.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background/aim: Dermatophytes are the causative agents of dermatophytosis, which is a common disease worldwide that affects thehair, skin, and nails. Dermatophytes comprise more than 40 species in 3 genera: Microsporum, Trichophyton, and Epidermaphyton. Inthis study, we aimed to determine the effectiveness of seven antifungal agents: amphotericin B, terbinafine, itraconazole, voriconazole,ketoconazole, miconazole, and fluconazole.Materials and methods: A sensitivity study was performed using a microdilution method in accordance with the CLSI M38-A2standards using isolates of Trichophyton rubrum (n = 55), Microsporum canis (n = 9), and Trichophyton interdigitale (n = 2), which wereidentified by sequencing the internal transcribed spacer region of the rDNA.Results: According to the results of antifungal sensitivity tests, the geometric mean (GM) minimum inhibitory concentration (MIC)against T. rubrum was 0.10 μg/mL for ketoconazole, 0.20 μg/mL for itraconazole, 0.07 μg/mL for miconazole, 0.48 μg/mL for fluconazole,2.27 μg/mL for amphotericin B, 0.06 μg/mL for voriconazole, and 0.06 μg/mL for terbinafine.

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Cite This Article

BARIŞ, A., ŞEN, S., ÖZAKKAŞ, F., ALTINBAŞ, R., TURAN, D. (2022). In vitro susceptibility of seven antifungal agents against dermatophytes isolated in İstanbul. Turkish Journal of Medical Sciences, Cilt: 48 - Sayı: 3, 615–619. https://ajindex.com/articles/610302

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