AI Summary & Key Takeaways
AI InsightAlso, regarding the coculture with embryo transfer, moderate leptin receptor immunoreactivity was seenin the cytoplasm and strong leptin receptor immunoreactivity was seen in the cell membrane.Conclusion: Embryo transfer to endometrium coculture triggers leptin receptor expression.
📌 3 Key Takeaways
Abstract
Background/aim: A synchronized dialogue between maternal and embryonic tissues is required for successful implantation. Lowuterine receptivity is responsible for two-thirds of implantation failures and leptin is effective in the physiology of reproduction bybinding to specific receptors. In this study, we investigate leptin receptor expression in cases of embryo transfer to endometrial coculture.Materials and methods: Biopsy materials were taken from 20 females with indication for coculture application and were cultured in anappropriate medium after the epithelial cells were isolated. The grown cells were cultured in chamber slides as the first group. For thesecond group, day 3 embryo was added to chamber slides and the development was observed. The embryo was transferred 1 or 2 dayslater and other cells (after the transfer process) were used to form the second group. After fixation, immunohistochemical staining wasperformed with anti-leptin primary antibody.Results: Regarding the coculture without embryo transfer, moderate leptin receptor immunoreactivity was seen in the perinuclearregion and the cell membrane. Also, regarding the coculture with embryo transfer, moderate leptin receptor immunoreactivity was seenin the cytoplasm and strong leptin receptor immunoreactivity was seen in the cell membrane.Conclusion: Embryo transfer to endometrium coculture triggers leptin receptor expression.