AI Summary & Key Takeaways
AI InsightAfter culture period, lymphocyte proliferation and viability, the frequency of CD4+CD25+FoxP3+ T regulatory cells, IL-10 and TNF-α levels in the culture supernatants were measured via flow cytometry.Results: Our results demonstrated that DFSCs suppressed proliferation of T lymphocytes by increasing the number of FoxP3 expressingCD4+CD25+ T regulatory cells and suppressed lymphocyte apoptosis in RA patients.
📌 3 Key Takeaways
Abstract
Background/aim: Multipotent mesenchymal stem cells (MSCs) have been investigated in autoimmune diseases such as rheumatoidarthritis (RA) due to their immunomodulatory and regenerative properties. In this study, their immunosuppressive effects on peripheralblood mononuclear cells (PBMC) of RA patients were studied.Materials and methods: Dental follicle stem cells (DFSCs) were isolated from follicle tissue in the orofacial region. Characterizationand multipotency analyses were performed. Lymphocytes were isolated from peripheral venous blood of RA patients (n = 5) andhealthy individuals (n = 5). DFSCs were preincubated with IFN-γ for 48 h. PBMCs of RA patients and healthy individuals wereseparately cultured with or without DFSCs for 72 h. After culture period, lymphocyte proliferation and viability, the frequency of CD4+CD25+FoxP3+ T regulatory cells, IL-10 and TNF-α levels in the culture supernatants were measured via flow cytometry.Results: Our results demonstrated that DFSCs suppressed proliferation of T lymphocytes by increasing the number of FoxP3 expressingCD4+CD25+ T regulatory cells and suppressed lymphocyte apoptosis in RA patients. Also, DFSCs reduced TNF-α cytokine secretionand upregulated IL-10 secreting cells.Conclusions: Such cells could potentially be a source for future immunomodulatory treatments of RA patients.