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Journals / Turkish Journal of Medical Sciences / 2019 / Cilt: 49 - Sayı: 2

Antibiotic resistance pattern and spa types of Staphylococcus aureus strains isolated from food business and hospital kitchen employees in Çanakkale, Turkey

Nesrin ÇAKICI
Nükhet Nilüfer DEMİREL ZORBA
Alper AKÇALI
Pages
675–682
Publication Date
2022-11-05
DOI
—
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All MRSA were determined to be SCCmec type IVa.Conclusion: spa typing can be performed to screen for transmission of S. aureus. t786, ST88, and SCCmec IVa MRSA strains wereidentified for the first time in Turkey

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Objective & Hypothesis:Background/aim: The aim of this study was to determine antibiotic resistance profiles and spa types of Staphylococcus aureus strainsisolated from food business employees in Çanakkale, Turkey.Materials and methods: S. aureus isolates were collected from hand and nasal swabs of 300 individuals working in 17 food businessesand 9 hospital kitchens.
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Key Findings & Data:All S. aureus isolates obtained from each carrier were typed by staphylococcal protein A (spa) typing method.Staphylococcal cassette chromosome mec (SCCmec) and multilocus sequence typing (MLST) of MRSA were performed by sequencingmethod.Results: Of the 300 individuals, 125 (41.6%) were found to be carriers of S. aureus, 215 isolates of which were obtained in total.
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Conclusion & Impact:All MRSA were determined to be SCCmec type IVa.Conclusion: spa typing can be performed to screen for transmission of S. aureus. t786, ST88, and SCCmec IVa MRSA strains wereidentified for the first time in Turkey
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background/aim: The aim of this study was to determine antibiotic resistance profiles and spa types of Staphylococcus aureus strainsisolated from food business employees in Çanakkale, Turkey.Materials and methods: S. aureus isolates were collected from hand and nasal swabs of 300 individuals working in 17 food businessesand 9 hospital kitchens. All S. aureus isolates obtained from each carrier were typed by staphylococcal protein A (spa) typing method.Staphylococcal cassette chromosome mec (SCCmec) and multilocus sequence typing (MLST) of MRSA were performed by sequencingmethod.Results: Of the 300 individuals, 125 (41.6%) were found to be carriers of S. aureus, 215 isolates of which were obtained in total. Three(1.4%) of 215 isolates were identified as MRSA. Sixty spa types were identified among the 121 MSSA isolates, the most common beingt084 (9%). A novel spa type was discerned and added to the Ridom SpaServer database as t14963. The MLST type of the MRSA strainsidentified as spa type t786 was ST88 and as spa type t223 was ST22. All MRSA were determined to be SCCmec type IVa.Conclusion: spa typing can be performed to screen for transmission of S. aureus. t786, ST88, and SCCmec IVa MRSA strains wereidentified for the first time in Turkey

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Cite This Article

ÇAKICI, N., ZORBA, N. N. D., AKÇALI, A. (2022). Antibiotic resistance pattern and spa types of Staphylococcus aureus strains isolated from food business and hospital kitchen employees in Çanakkale, Turkey. Turkish Journal of Medical Sciences, Cilt: 49 - Sayı: 2, 675–682. https://ajindex.com/articles/609968

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