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Journals / Turkish Journal of Hematology / 2020 / Cilt: 37 - Sayı: 2

Expression Profile Screening and Bioinformatics Analysis of circRNA, LncRNA, and mRNA in Acute Myeloid Leukemia DrugResistant Cells

Akut Myeloid Lösemi İlaç-Dirençli Hücrelerde circRNA, LncRNA, ve mRNA Ekspresyon Profili Tarama ve Biyoinformatik Analizi

Meiling Lİ
Quanyi LU
Fuxue MENG
Pages
104–110
Publication Date
2022-11-06
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The mTOR signaling pathway, MAPK signaling pathway,RAP1 signaling pathway, and Akt signaling pathway were closelyrelated to drug resistance.Conclusion: Our study provides a systematic outlook on the potentialfunction of ncRNA in the molecular mechanisms of resistant AML cells.Hsa-circ-0000978 and hsa-circ-0000483 might serve as potentialprognostic biomarkers and therapeutic targets of AML resistance.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Acute myeloid leukemia (AML) is a highly heterogeneoushematological malignancy, and drug resistance and relapse arekey factors in the failure of leukemia treatment.
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Key Findings & Data:Studies haveincreasingly shown that circRNA and LncRNA play important rolesin the development of tumors, but their roles remain unclear in themechanism of AML resistance.Materials and Methods: Resistant AML cell line HL-60/ADM(adriamycin, ADM) was constructed and circRNA, LncRNA, and mRNAexpression profiles were screened followed by high-throughputsequencing.
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Conclusion & Impact:The mTOR signaling pathway, MAPK signaling pathway,RAP1 signaling pathway, and Akt signaling pathway were closelyrelated to drug resistance.Conclusion: Our study provides a systematic outlook on the potentialfunction of ncRNA in the molecular mechanisms of resistant AML cells.Hsa-circ-0000978 and hsa-circ-0000483 might serve as potentialprognostic biomarkers and therapeutic targets of AML resistance.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Acute myeloid leukemia (AML) is a highly heterogeneoushematological malignancy, and drug resistance and relapse arekey factors in the failure of leukemia treatment. Studies haveincreasingly shown that circRNA and LncRNA play important rolesin the development of tumors, but their roles remain unclear in themechanism of AML resistance.Materials and Methods: Resistant AML cell line HL-60/ADM(adriamycin, ADM) was constructed and circRNA, LncRNA, and mRNAexpression profiles were screened followed by high-throughputsequencing. Bioinformatics analysis was then carried out, and thecircRNA-miRNA ceRNA network was constructed and confirmed usingqRT-PCR analysis.Results: A total of 1824 circRNAs, 2414 LncRNAs, and 5346 mRNAswere screened for differentially expressed genes. Enrichment analysiswas performed utilizing Gene Ontology and the Kyoto Encyclopedia ofGenes and Genomes, which mainly involved protein domain specificbinding, transforming growth factor-β (TGF-β) receptor, and cellularmetabolism. The mTOR signaling pathway, MAPK signaling pathway,RAP1 signaling pathway, and Akt signaling pathway were closelyrelated to drug resistance.Conclusion: Our study provides a systematic outlook on the potentialfunction of ncRNA in the molecular mechanisms of resistant AML cells.Hsa-circ-0000978 and hsa-circ-0000483 might serve as potentialprognostic biomarkers and therapeutic targets of AML resistance.

Özet

Amaç: Akut myeloid lösemi (AML) oldukça heterojen bir hematolojik malignitedir, ve tedavi başarısızlığında ilaç direnci ve nüks anahtar rol oynamaktadır. Çalışmalar, circRNA ve lncRNA’nın tümör gelişiminde önemli rol oynadığını artarak göstermektedir, ancak AML direnç mekanizmasında rolleri belirsizliğini korumaktadır. Gereç ve Yöntemler: Dirençli AML hücre hattı HL-60/ADM (adriamisin, ADM) oluşturuldu ve circRNA, LncRNA, ve mRNA ekspresyon profilleri yüksek-kapasitede dizileme sonrası tarandı. Sonra biyoinformatik analiz gerçekleştirildi ve circRNA-miRNA ceRNA ağı oluşturuldu ve qRT-PCR analizi kullanılarak doğrulandı. Bulgular: Farklı ifade edilen genler için toplam 1824 circRNA, 2414 LncRNA, ve 5346 mRNA tarandı. Başlıca protein domain spesifik bağlama, transforme edici büyüme faktörü-β (TGF-β) reseptörü, ve hücresel metabolizma ile ilgili ‘Gene Ontology’ ve ‘Kyoto Encyclopedia of Genes and Genome’ kullanılarak zenginleştirme analizi gerçekleştirildi. mTOR sinyal yolağı, MAPK sinyal yolağı, RAP1 sinyal yolağı ve Akt sinyal yolağı ilaç direnci ile yakından ilişkili idi. Sonuç: Çalışmamız, dirençli AML hücrelerinin moleküler mekanizmasında ncRNA’ın potansiyel fonksiyonuna sistematik bir bakış açısı sağlamıştır. Hsa-circ-0000978 ve hsa-circ-0000483 potansiyel prognostik biyogöstergeler ve AML direncinin terapötik hedefleri olarak işlev görebilirler.

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Cite This Article

Lİ, M., LU, Q., MENG, F. (2022). Expression Profile Screening and Bioinformatics Analysis of circRNA, LncRNA, and mRNA in Acute Myeloid Leukemia DrugResistant Cells. Turkish Journal of Hematology, Cilt: 37 - Sayı: 2, 104–110. https://ajindex.com/articles/606639

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References (12)

  1. Li XX, Zhou JD, Wen XM, Zhang TJ, Wu DH, Deng ZQ, Zhang ZH, Lian XY, He PF, Yao XY, Lin J, Qian J. Increased MCL-1 expression predicts poor prognosis and disease recurrence in acute myeloid leukemia. Onco Targets Ther 2019;12:3295-3304
  2. Shang J, Chen WM, Liu S, Wang ZH, Wei TN, Chen ZZ, Wu WB. CircPAN3 contributes to drug resistance in acute myeloid leukemia through regulation of autophagy. Leuk Res 2019;85:106198
  3. Lai QY, He YZ, Peng XW, Zhou X, Liang D, Wang L. Histone deacetylase 1 induced by neddylation inhibition contributes to drug resistance in acute myelogenous leukemia. Cell Commun Signal 2019;17:86
  4. Bertone P, Stolc V, Royce TE, Rozowsky JS, Urban AE, Zhu X, Rinn JL, Tongprasit W, Samanta M, Weissman S, Gerstein M, Snyder M. Global identification of human transcribed sequences with genome tiling arrays. Science 2004;306:2242-2246
  5. Kapranov P, Cheng J, Dike S, Nix DA, Duttagupta R, Willingham AT, Stadler PF, Hertel J, Hackermüller J, Hofacker IL, Bell I, Cheung E, Drenkow J, Dumais E, Patel S, Helt G, Ganesh M, Ghosh S, Piccolboni A, Sementchenko V, Tammana H, Gingeras TR. RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science 2007;316:1484-1488
  6. Guttman M, Rinn JL. Modular regulatory principles of large noncoding. Nature 2012;482:339-346
  7. Li Z, Zhao X, Zhou Y, Liu Y, Zhou Q, Ye H, Wang Y, Zeng J, Song Y, Gao W, Zheng S, Zhuang B, Chen H, Li W, Li H, Li H, Fu Z, Chen R. The long noncoding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer. J Transl Med 2015;13:84
  8. Li Z, Zhao L, Wang Q. Overexpression of long non-coding RNA HOTTIP increases chemoresistance of osteosarcoma cell by activating the Wnt/β- catenin pathway. Am J Transl Res 2016;8:2385-2393
  9. Qu L, Ding J, Chen C, Wu ZJ, Liu B, Gao Y, Chen W, Liu F, Sun W, Li XF, Wang X, Wang Y, Xu ZY, Gao L, Yang Q, Xu B, Li YM, Fang ZY, Xu ZP, Bao Y, Wu DS, Miao X, Sun HY, Sun YH, Wang HY, Wang LH. Exosome-transmitted lncARSR promotes sunitinib resistance in renal cancer by acting as a competing endogenous RNA. Cancer Cell 2016;29:653-668
  10. Wilusz JE, Sharp PA. Molecular biology. A circuitous route to noncoding RNA. Science 2013;340:440-441
  11. Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF, Sharpless NE. Circular RNAs are abundant, conserved, and associated with ALU repeats. RNA 2013;19:141-157
  12. Grisendi S, Mecucci C, Falini B, Pandolfi PP. Nucleophosmin and