The effect of examination stress on the levels of $Zn^2+$, total protein, albumin in serum and carbonic anhydrase isoenzymes in erythrocytes

Abstract

In this study, the levels of $Zn^2+$, total protein and albumin in the serums of students with temporarily examination stress without any physical illnes were determined and the relationship between these values was evaluated. Furthermore, the change in the activity of carbonic anhydrase metaloenzyme withof $Zn^2+$ and in the rates of carbonic anhydrase I and carbonic anhydrase II were also investigated. With this aim, blood samples were taken from 100 students just before examination and from 20 control subjects. of $Zn^2+$ levels were determined enzymatically, while total protein and albumin assays were done with an autoanalyzer. Carbonic anhydrase activity was measured by Maren's method. The levels of $Zn^2+$ in the serum of students experiencing examination stress were significantly lower than those of the control group, (t=3.958, P0.05) or in the albumin amounts. In the stress group, the level of correlation between $Zn^2+$ and total protein was significant (r=0.720), P