Journals / Turkish Journal of Biology / 1998 / Cilt: 22 - Sayı: 1

The changes of the localization of acorbic in the rat adrenal gland on microwave applied

Mikrodalga uygulanmış ratların böbreküstü bezlerinde askorbik asit'in lokalizasyonundaki değişiklikler

Pages
43–51
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the reason or the increase of ascorbic acid granulles in the adrenal glands of the rast to which microwave was applied for long duration, is likely to be due to the increase of body resistance against stress response.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The purpose of this study was to investigate the effect of microwave on the localization of ascorbic acid in the rat adrenal gland.
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Key Findings & Data:We studies on 21 male Spraque-Dawley rast (220-250 g), aged 2.5 month.
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Conclusion & Impact:In conclusion, the reason or the increase of ascorbic acid granulles in the adrenal glands of the rast to which microwave was applied for long duration, is likely to be due to the increase of body resistance against stress response.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The purpose of this study was to investigate the effect of microwave on the localization of ascorbic acid in the rat adrenal gland. We studies on 21 male Spraque-Dawley rast (220-250 g), aged 2.5 month. The animals were divided into three different groups. First group was taken on control (n:7). The second group (n:7) 9450 MHz, 2.65 mW/cm2 was applied for one hour a day for 13 days, while in the third group (n:7) this application continued for 52 days. Rast were sacrified under ether anaesthesia and then their adrenal gland were removed enbloc and fixed in silver nitrate solution (10 %). The paraffin sections were stained with Alum Carmen and evaluated under the ligth microscope. In group I, the ascorbic acid granules formed a homogen distribution, whereas in the second group the ascorbic acid in the capsula capiller endothelium and zona glomerulosa of adrenal gland does not exit, but a different formation was observed in the fasciulata, reticularis and medulla. One the other hand, in group III animals the ascorbic acid granules being in accumulation was clearly seen in the endothelium of the capiller sinuzoids. In conclusion, the reason or the increase of ascorbic acid granulles in the adrenal glands of the rast to which microwave was applied for long duration, is likely to be due to the increase of body resistance against stress response.

Özet

Bu çalısmanın amacı, mikrodalga uygulanan ratların böbreküstü bezlerinde askorbik asitin lokalizasyonunu histosimlik yöntemlerle arastırmaktadır. Çalısmamızda, 21 adet 220-250 g agırlıgında 10 haftalık erkek Spraque-Dawley rat kullanıldı. Deney hayvanları üç gruba bölündü. Grup-I’deki deney hayvanları kontrol grubu olarak kullanıldı (n:7). Grup-II’deki ratlara 13 gün süreyle, günde 1 saat 9450 MHz frekansında, 2,65 mW/cm2 dozunda mikrodalga uygulandı (n:7). Grup-III’deki ratlara 52 gün süreyle, günde 1 saat 9450 MHz frekansında, 2,65 mW/cm2 dozunda mikrodalga uygulandı (n:7). Deney bitiminde, eter anestezisi altında sakrifiye edilen ratların, böbreküstü bezleri alınarak %10'luk gümüs nitratla fikse edildi. Parafin bloklardan elde edilen kesitler alum carmen ile boyanarak ısık mikroskobu altında degerlendirildi. GrupI’de askorbik asit granülleri homojen bir dagılım gösterirken, Grup-II’deki böbreküstü bezlerinin, kapsula kapiller endoteli ve zona glomeruloza askorbik asitten tamamen yoksun olmasına karsın; fasikulata, retikülaris ve medulla askorbik asit yönünden farklı formasyonlar göstermekteydi. Grup-III’ün böbreküstü bezlerinde ise organın kapsulası dahi her tarafta bulunan askorbik asit granülleri farklı çap ve yogunlukta izlendi. Özellikle kapiller sinüzoidlerin endotellerinde bu akümülasyon çok barizdi. Sonuç olarak; uzun süreli mikrodalga uygulanan ratların böbreküstü bezlerinde askorbik asit granüllerinde artıs, strese karsı vücut direncini artırmaya yönelik oldugu kanaatine varıldı.

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Cite This Article

NERGİZ, Y., AKDAĞ, Z., ÇELİK, M., ŞAHAN, Ö., KETANİ, M. (2022). The changes of the localization of acorbic in the rat adrenal gland on microwave applied. Turkish Journal of Biology, Cilt: 22 - Sayı: 1, 43–51. https://ajindex.com/articles/600660

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