SavedAdd your journal

Journals / Turkish Journal of Biology / 2002 / Cilt: 26 - Sayı: 3

Tavşanlarda deneysel diyabetin plazma laktat dehidrogenaz ve glutamik oksalasetik transaminaz enzim düzeylerine etkisi

Effect of experimental diabetes mellitus on plasma lactate deydrogenase and glutamic oxaloacetic transaminase levels in rabbits

Fehmi ODABAŞOĞLU
İsmail ÇELİK
Eşref YEĞİN
Pages
151–154
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, we agree that routine use of LDH measurement to monitor diabetic status may be useful, but the characteristic parameters of diabetes mellitus are better indicators.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Hyperglycemia was induced in 20 male, white, New Zealand rabbits (2-3 kg) (Oryctolagus cuniculus huxley) by alloxan (100 mg/kg body weight, I.V.) and maintained for 16 weeks without insulin treatment.
📊
Key Findings & Data:The levels of LDH and GOT were 155.6 $pm$ 41.9 U/L and 47.0 $pm$ 20.4 U/L in the control group respectively.
💡
Conclusion & Impact:In conclusion, we agree that routine use of LDH measurement to monitor diabetic status may be useful, but the characteristic parameters of diabetes mellitus are better indicators.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Hyperglycemia was induced in 20 male, white, New Zealand rabbits (2-3 kg) (Oryctolagus cuniculus huxley) by alloxan (100 mg/kg body weight, I.V.) and maintained for 16 weeks without insulin treatment. Blood samples were taken from both diabetic and non-diabetic rabbits at the same time for measurement of plasma enzymes. Levels of glutamic oxalacetic transaminase (GOT) and lactate dehydrogenase (LDH) were determined after treatment. The levels of LDH and GOT were 155.6 $pm$ 41.9 U/L and 47.0 $pm$ 20.4 U/L in the control group respectively. These parameters were 286.9 $pm$ 142.9 U/L and 93.9 $pm$62.5 U/L in the diabetic group respectively. It was observed that LDH (p 0.05) in the diabetic group. In conclusion, we agree that routine use of LDH measurement to monitor diabetic status may be useful, but the characteristic parameters of diabetes mellitus are better indicators.

Özet

Bu çalışmada, diabet 20 erkek, beyaz, Yeni Zelanda ırkı tavşan (Oryctolagus cuniculus huxley) alloxan verilerek (100 mg/kg vucut i.v.) oluşturuldu ve insulin tedavisi yapılmaksızın 16 hafta bekletildi. Plazma enzimlerini ölçümü için kan örnekleri hem diabetik hemde kontrol grublarından alındı. Muameleden sonra laktat dehidrogenaz (LDH) ve glutamik oksalasetik transaminaz (GOT) seviyeleri belirlendi. LDH ve GOT seviyeleri sırasıyla, kontrol grubunda 155,6 ± 41,9 U/L ve 47,0 ± 20,4 U/L iken, diabetli grubunda 286,9 ± 142,9 U/L ve 93,9 ± 62,5 U/L idi. Yapılan istatistiki analiz sonucu, diabetli, plazma LDH değerinin önemli oranda (p 0,05) görüldü. Sonuç olarak, LDH diabetes mellitus için bir indikatör olabilirler ancak, diabetes mellitus’un mevcut karakteristik paremetreleri daha iyi birer indikatördürler.

🔖

Cite This Article

ODABAŞOĞLU, F., ÇELİK, İ., YEĞİN, E. (2022). Tavşanlarda deneysel diyabetin plazma laktat dehidrogenaz ve glutamik oksalasetik transaminaz enzim düzeylerine etkisi. Turkish Journal of Biology, Cilt: 26 - Sayı: 3, 151–154. https://ajindex.com/articles/600578

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Stewart, M. Animal Physiology, Kent UK, 1991, Holder and Stough Ltd., 313-7
  2. Leninger, A.L. Principles of Biochemistry, New York, 1982, Worth Publishers Inc., 712-14
  3. Oliver, R.C., Tervonen, T., Flynn, D.G., Keenan, K.M. Enzyme activity in crevicular fluid in relation to metabolic control of diabetes and other periodontal risk factors. J. Periodontal. 91(5): 423-6, 1993
  4. Rossi, I., Sanchez-Arias, J.A., Feliu, J.E. Effect of streptozotocin diabetes on glycolytic flux and on fructose 2,6 biphosphate level in isolated rat enterocytes. Metabolism, 39(8): 882-5, 1990
  5. Melinkeri, R.R., Sontakke, A.N., Kulkarni, A.M. Serum zinc and zinc containing enzymes in diabetes mellitus. Indian J. Med. Sci, 44(7): 173-7, 1990
  6. Awaji, Y., Hastimoto, H., Matsui, Y., Kawaguchi, K., Okumura, K., Ito, T., Stake, T. Isoenzyme profiles of creatine kinase, lactate dehydrogenase and aspartate aminotransferase in diabetic heart: comparison with herditary and catecholamine cardiomyopathies. Cardivasc Res, 24(7): 547-54, 1990
  7. Cai, F. Studies of enzymes histochemistry and ultrastructure of myocardium in rats with streptozotocin-induced diabetes. Zhonghua Yi Xue Za Zhi, 69(5): 276-8, 1989
  8. Nikolaeva, L.F., Azmukhanbetova, A.Z., Titov, V.N., Perelygina, A.A. Value of determining serum enzyme activity during the exercise test in evaluating the functional condition of patients with ischemic heart disease and concomitant diabetes mellitus. Kardiologiia, 28(11): 85-90, 1988
  9. Tanaka, T., Nanbara, S., Tanaka, T. Koide, H., Hayashi, T. Aminotransferase activity in liver of diabetic mice. Diabetes Res. Clin. Pract, 19: 71-5, 1988
  10. Jones, R.G., Grant, P.G., Brown, D., Stickland, M., Wiles, P.G. A rise in the plasma activites of hepatic enzymes is not a common consequence of hypoglycaemia. Diabetes Med, 5(3): 253-5, 1988
  11. Ryder, E., Campos, G., Morales-Villalobos, L.M. Enzymatic changes in polymorphonuclear cells isolated from type II diabetics. Biochem. Med. Metab. Biol, 37(2): 205-12, 1987
  12. Margiavichene, L.E., Gribausckas, P.S., Norkus, A.V., Gribauskene R.A., Masalskene, V.V. Lipid metabolism and the activity of cardiospecific enzymes in diabetes mellitus. Probl. Endokrinol (Mosk), 32(5): 28-32, 1986
  13. Cheshchevik A.B. Energy metabolism in the kidney cortex of rats with alloxan diabetes. Vopr. Med. Khim, 31(1): 52-54, 1985
  14. Zappacosta, B., De Sole, P., Rossi, C., Marra, G., Ghirlanda, G., Giardina, B. Lactate dehydrogenase activiy of platelets in d