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Journals / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 6

Preparation and characterization of acellular porcine pericardium for cardiovascular surgery

Ha Le Bao TRAN
Trang Thi Huyen DINH
Öy Thi Ngoc NGUYEN
Quan Minh TO
Anh Tho Tuan PHAM
Pages
1243–1250
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not cause a local inflammatory effect after 30 days of transplantation in mice.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair.
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Key Findings & Data:Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals.
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Conclusion & Impact:Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not cause a local inflammatory effect after 30 days of transplantation in mice.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair. Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals. The optimal decellularization protocol was determined according to elimination of cellular components and DNA via histology and DNA quantification, respectively. There were no significant changes in stress and fracture strain after decellularization. Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not cause a local inflammatory effect after 30 days of transplantation in mice.

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Cite This Article

TRAN, H. L. B., DINH, T. T. H., NGUYEN, Ö. T. N., TO, Q. M., PHAM, A. T. T. (2022). Preparation and characterization of acellular porcine pericardium for cardiovascular surgery. Turkish Journal of Biology, Cilt: 40 - Sayı: 6, 1243–1250. https://ajindex.com/articles/600404

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