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Journals / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 6

Higher expression level of Bat3 is associated with silencing of the Midn gene in primary mouse cardiomyocytes

Evrim BAYRAK KÖMÜRCÜ
Bilge SELÇUK ÖZSAİT
Nihan ÜNALTUNA ERGİNER
Pages
1295–1302
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, we have detected a suggestive gene that might act in the common cellular pathways with Midn in cardiomyocyte cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Midnolin (Midn) is a developmentally regulated nucleolar protein that was originally identified in the mesencephalon of developing mouse embryos.
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Key Findings & Data:After 24 h of silencing, we observed that the expression level of Bat3 was significantly increased (1.69-fold, P = 0.04).
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Conclusion & Impact:As a result, we have detected a suggestive gene that might act in the common cellular pathways with Midn in cardiomyocyte cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Midnolin (Midn) is a developmentally regulated nucleolar protein that was originally identified in the mesencephalon of developing mouse embryos. Additionally, Midn expression was detected in various adult tissues and was associated with glucokinase activity in pancreatic beta cells and with the regulation of folliculogenesis in ovaries. In this study, our aim was to analyze the expression and function of the Midn gene in cardiomyocyte cells. We constructed a primary neonatal mouse cardiomyocyte cell culture and analyzed the expression of possible Midn-associated genes after short interfering RNA (siRNA)-mediated gene silencing for Midn. After 24 h of silencing, we observed that the expression level of Bat3 was significantly increased (1.69-fold, P = 0.04). As a result, we have detected a suggestive gene that might act in the common cellular pathways with Midn in cardiomyocyte cells.

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Cite This Article

KÖMÜRCÜ, E. B., ÖZSAİT, B. S., ERGİNER, N. Ü. (2022). Higher expression level of Bat3 is associated with silencing of the Midn gene in primary mouse cardiomyocytes. Turkish Journal of Biology, Cilt: 40 - Sayı: 6, 1295–1302. https://ajindex.com/articles/600368

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