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Journals / Turkish Journal of Biology / 2017 / Cilt: 41 - Sayı: 1

Cryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen

Muammer CEYLAN
Ergun KAYA
Emel GÖKDOĞAN YILMAZ
Fernanda Vidigal Duerte SOUZA
Maria M. JENDEREK
Pages
242–248
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation.
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Key Findings & Data:The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck.
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Conclusion & Impact:Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability ranged from 73.3% (Poncirus trifoliata Raf. × C. sinensis Osb. and Fortunella margarita (Lour.) Swingle) to 93.3% (C. jambhiri Lush.). Dehydration was beneficial for germination of seeds from all of the tested citrus species after the liquid nitrogen exposure. Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.

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Cite This Article

CEYLAN, M., KAYA, E., YILMAZ, E. G., SOUZA, F. V. D., JENDEREK, M. M. (2022). Cryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen. Turkish Journal of Biology, Cilt: 41 - Sayı: 1, 242–248. https://ajindex.com/articles/600319

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