SavedAdd your journal

Journals / Turkish Journal of Biology / 2017 / Cilt: 41 - Sayı: 6

Overexpression of the IbOr gene from sweet potato ( Ipomea batatasHoang Long') in maize increases total carotenoid and β-carotene contents

Thi-Luong TRAN
Duc-Thanh NGUYEN
Thi-Huong HO
Pages
1003–1010
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the total carotenoid and β-carotene content of all analyzed transgenic maizeplants were significantly higher than those of wild-type lines.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitaminA carotenoids including β-carotene, α-carotene, and β-cryptoxanthin.
📊
Key Findings & Data:In this study, we report the successful overexpression of theIbOrgene in H145 and H95 inbred maize lines under the control of maize seed-specific promoter globulin 1 (Glo1) for the purpose ofimproving β-carotene in maize.
💡
Conclusion & Impact:The results showed that the total carotenoid and β-carotene content of all analyzed transgenic maizeplants were significantly higher than those of wild-type lines.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitaminA carotenoids including β-carotene, α-carotene, and β-cryptoxanthin. In this study, we report the successful overexpression of theIbOrgene in H145 and H95 inbred maize lines under the control of maize seed-specific promoter globulin 1 (Glo1) for the purpose ofimproving β-carotene in maize. The results showed that the total carotenoid and β-carotene content of all analyzed transgenic maizeplants were significantly higher than those of wild-type lines. For H145-IbOr transgenic maize, in the best line (H145-IbOr.10), the totalcarotenoid and β-carotene contents were increased up to 10.36- and 15.11-fold, respectively, compared to the wild type (H145-WT). Inthe case of H95-IbOr transgenic plants, 5.58-fold increase in total carotenoid and 7.63-fold increase in β-carotene were achieved in theH95-IbOr.6 line compared to nontransgenic plants (H95-WT). In all the transgenic plants derived from the wild-type maize line withless carotenoid content (H145-WT), the content of both total carotenoid and β-carotene was higher than in transgenic plants derivedfrom the wild-type maize line having more carotenoid content (H95-WT). Our research is the first in successful overexpression ofIbOr gene in maize.

🔖

Cite This Article

TRAN, T., NGUYEN, D., HO, T. (2022). Overexpression of the IbOr gene from sweet potato ( Ipomea batatasHoang Long') in maize increases total carotenoid and β-carotene contents. Turkish Journal of Biology, Cilt: 41 - Sayı: 6, 1003–1010. https://ajindex.com/articles/600279

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Al Babili S, Hartung W, Kleinig H, Beyer P (1999). CPTA modulates levels of carotenogenic proteins and their mRNAs and affects carotenoid and ABA content as well as chromoplast structure in Narcissus pseudonarcissus flowers. Plant Biol 1: 607-612.
  2. Aluru M, Xu Y, Guo R,Wang Z G, Li SS, White W, Wang K, Rodermel S (2008). Generation of transgenic maize with enhanced provitamin content. J Exp Bot 59: 3551-3562.
  3. Bai C, Rivera S, Medina V, Alves R, Vilaprinio E, Sorribas A, Canela R, Capell T, Sandmann G, Christou P et al. (2014). An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation. Plant J 77: 464-475.
  4. Bai C, Capell T, Berman J, Medina V, Sandmann G, Christou P, Zhu C (2016). Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor–product balance. Plant Biotech J 14: 195-205.
  5. Berman J, Zorrilla-López U, Medina V, Farré G, Sandmann G, Capell T, Christou P, Zhu C (2017). The Arabidopsis ORANGE ( AtOR ) gene promotes carotenoid accumulation in transgenic corn hybrids derived from parental lines with limited carotenoid pools. Plant Cell Rep 36: 933-945.
  6. Burkhardt PK, Beyer P, Wunn J, Kloti A, Armstrong GA, Schledz M, von Lintig J, Potrykus I (1997). Transgenic rice ( Oryza sativa ) endosperm expressing daffodil ( Narcissus pseudonarcissus ) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J 11: 1071-1078.
  7. Cho KS, Han EH, Kwak SS, Cho JH, Im JS, Hong SY, Sohn HB, Kim YH, Lee SW (2016). Expressing the sweet potato orange gene in transgenic potato improves drought tolerance and marketable tuber production. C R Biol 339: 207-213.
  8. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975). Establishment of an efficient medium for another culture of rice through comparative experiments of the nitrogen sources. Sci Sin 18: 659-668.
  9. Cookson PJ, Kiano JW, Shipton CA, Fraser PD, Romer S, Schuch W, Bramley P, Pyke KA (2003). Increases in cell elongation, plastid compartment size and phytoene synthase activity underlie the phenotype of the high pigment-1 mutant of tomato. Planta 217: 896-903.
  10. Diretto G, Al-Babili S, Tavazza R, Papacchioli V, Beyer P, Giuliano G (2007). Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini- pathway. PLoS ONE 2: e350. Goo YM, Han EH, Jeong JC, Kwak SS, Yu J, Kim YH, Ahn MJ, Lee SW (2015). Overexpression of the sweet potato