SavedAdd your journal

Journals / Turkish Journal of Agriculture and Forestry / 2014 / Cilt: 38 - Sayı: 4

A highly efective nucleopolyhedrovirus against Malacosoma spp. (Lepidoptera: Lasiocampidae) from Turkey: isolation, characterization, phylogeny, and virulence

Zihni DEMİRBAĞ
İsmail DEMİR
Remziye NALÇACIOĞLU
Lida GHOLİZAD MOHAMMAD
Pages
462–470
Publication Date
2022-10-19
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results indicate that the new virus is anotherisolate of ManeNPV that infects M. americanum larvae naturally.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:A nucleopolyhedrovirus (NPV) was isolated from diseased larvae of the eastern tent caterpillar, Malacosoma americanum(Fabricius), collected from the northeastern part of Turkey.
📊
Key Findings & Data:Te dimensions of the polyhedral inclusion bodies varied from 1.0 to 2.1 µm, and the sizesof the virions were also measured to be approximately 194.5 nm.
💡
Conclusion & Impact:Our results indicate that the new virus is anotherisolate of ManeNPV that infects M. americanum larvae naturally.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

A nucleopolyhedrovirus (NPV) was isolated from diseased larvae of the eastern tent caterpillar, Malacosoma americanum(Fabricius), collected from the northeastern part of Turkey. Light and electron microscopic observations showed that this virus is amultiple nucleopolyhedrovirus (MNPV). Te dimensions of the polyhedral inclusion bodies varied from 1.0 to 2.1 µm, and the sizesof the virions were also measured to be approximately 194.5 nm. Restriction analysis of the new isolate and ManeNPV-T2 (previouslyisolated from M. neustria larvae) showed that the new virus is closely related to ManeNPV-T2. Furthermore, sequence and phylogeneticanalysis of polh and lef-8 also revealed a high degree of homology to M. neustria NPV. Our results indicate that the new virus is anotherisolate of ManeNPV that infects M. americanum larvae naturally. Terefore, it was named ManeNPV-T3. Te virulence of ManeNPV-T3,which was tested against the third instar of M. americanum and M. neustria larvae under laboratory conditions, indicated that 0.6 ×10 5 occlusion bodies per larvae caused 100% mortality within 10 days. Te same dose also showed viral infection in Malacosomacastrensis, Plodia interpunctella , Taumetopoea pityocampa, and Galleria mellonella larvae causing 65%, 60%, 35%, and 25% mortalities,respectively. Te fndings indicate that ManeNPV-T3 is a promising microbial agent for the biological control of Malacosoma species.

🔖

Cite This Article

DEMİRBAĞ, Z., DEMİR, İ., NALÇACIOĞLU, R., MOHAMMAD, L. G. (2022). A highly efective nucleopolyhedrovirus against Malacosoma spp. (Lepidoptera: Lasiocampidae) from Turkey: isolation, characterization, phylogeny, and virulence. Turkish Journal of Agriculture and Forestry, Cilt: 38 - Sayı: 4, 462–470. https://ajindex.com/articles/599473

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (1)

  1. Abbott WS (1925). A method of computing the efectiveness of an insecticide. J Econ Entomol 18: 265– 267. Ackermann HW, Smirnof WA (1983). A morphological investigation of 23 baculoviruses. J Invertebr Pathol 41: 269 –280. Biliotti E (1955). Survie des larves endophages de tachinaires à une mort prématurée de leur hôte par maladie. CR Acad Sci Paris 240: 1021–1023 (article in French). Demir İ, Gürel N, Nalçacıoğlu R, İnce İA, Demirbağ Z (2009a). Productive replication of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) in Md203 cell line. Turk J Biol 33: 239–248. Demir İ, Gürel N, Nalçacıoğlu R, Demirbağ Z (2009b). Comparative susceptibilities of six insect cell lines to infection by Malacosoma neustria nucleopolyhedrovirus (ManeNPV). Turk J Biol 33: 259–273. Demir İ, Nalçacıoğlu R, Demirbağ Z (2008). Te signifcance of insect viruses in biotechnology. Tarım Bilim Derg 14: 193–201 (article in Turkish with an abstract in English). Demir İ, Nalçacıoğlu R, Mohammad Gholizad L, Demirbağ Z (2013). Characterization of a new isolate of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey. Turk J Biol 37: 385–391. de Moraes RR, Maruniak JE (1997). Detection and identifcation of multiple baculoviruses using the polymerase chain reaction (PCR) and restriction endonuclease analysis. J Virol Methods 63: 209–217. Doğanlar M (1975). Erzurum bölgesinde önemli lepidopter tırtıllarında bulunan Tachinidae sinekleri ve bunların kısa biyolojileri. Atatürk Üniversitesi Yayınları, No. 375. Atatürk Üniversitesi Basımevi, Erzurum, 136 s. (article in Turkish). Günther S (1958). Eine bisher unbekannte Mikrosporidie aus dem Ringelspinner (Malacosoma neustria L.: Lasiocampidae). Z Pf Krankh 65: 534–535 (article in German). Herniou EA, Olszewski JA, O’Reilly DR, Cory JS (2004). Ancient coevolution of baculoviruses and their insect hosts. J Virol 78: 3244–3251. Ishii T, Nakai M, Okuna S, Takatsuka J, Kunimi Y (2003). Characterization of Adoxophyes honmai single-nucleocapsid nucleopolyhedrovirus: morphology, structure, and efects on larvae. J Invertebr Pathol 83: 206–214. Jankevica L, Cudare Z, Ose V (1998). New isolate of Malacosoma neustria nuclear polyhedrosis virus in Latvia. J Invertebr Pathol 71: 283–285. Jankevica L, Zarins I (1999). Virulance of Malacosoma neustria nucleopolyhedrovirus Latvian isolates. Latvijas Entomologs 37: 40–45. Keddie AB, Erlandson MA (1995). Characterization of a nuclear polyhedrosis virus from the forest tent caterpiller, Malacasoma disstria.