Journals / Turkish Journal of Agriculture and Forestry / 2017 / Cilt: 41 - Sayı: 5

Diversity and genetic analysis through DArTseq in common bean (Phaseolus vulgaris L.) germplasm from Turkey

Pages
389–404
Publication Date
2022-10-19
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study demonstrated that discovering SNPs from the whole genome is a potential resource for identifyingnaturally diverse accessions and also the information could be used in breeding programs to develop new common bean varieties.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The common bean (Phaseolus vulgaris L.) is the most consumed food legume in the world and is a major source of dietaryprotein, carbohydrates, and valuable micronutrients, especially in developing countries.
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Key Findings & Data:A total of 43,018 SNPs were identifiedfrom 173 common bean accessions, including Andean and Mesoamerican genotypes.
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Conclusion & Impact:This study demonstrated that discovering SNPs from the whole genome is a potential resource for identifyingnaturally diverse accessions and also the information could be used in breeding programs to develop new common bean varieties.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The common bean (Phaseolus vulgaris L.) is the most consumed food legume in the world and is a major source of dietaryprotein, carbohydrates, and valuable micronutrients, especially in developing countries. Diversity Arrays Technology (DArTseq),based on genome reduction with restriction enzymes, provides a rapid, high-throughput, and cost-effective tool capable of generatingthousands of genotyped single nucleotide polymorphisms (SNPs) for a genome-wide analysis of genetic diversity. In this study, weaimed to characterize common bean accessions using SNPs detected by a DArTseq approach. A total of 43,018 SNPs were identifiedfrom 173 common bean accessions, including Andean and Mesoamerican genotypes. After filtering raw and redundant data, a totalof 16,366 SNPs were considered for further analyses. According to population structure analysis, the genotypes were roughly dividedinto 2 gene pools of Andean and Mesoamerican types. Pairwise fixation index (Fst) values were calculated to resolve the differentiationbetween populations. This study demonstrated that discovering SNPs from the whole genome is a potential resource for identifyingnaturally diverse accessions and also the information could be used in breeding programs to develop new common bean varieties.

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Cite This Article

EŞİYOK, D., AŞÇIOĞUL, T. K., NEMLİ, S., ATEŞ, D., TANYOLAÇ, M. B. (2022). Diversity and genetic analysis through DArTseq in common bean (Phaseolus vulgaris L.) germplasm from Turkey. Turkish Journal of Agriculture and Forestry, Cilt: 41 - Sayı: 5, 389–404. https://ajindex.com/articles/599348

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References (8)

  1. Bajaj D, Das S, Badoni S, Kumar V, Singh M, Bansal KC, Tyagi AK, Parida SK (2014). Genome-wide high-throughput SNP discovery and genotyping for understanding natural (functional) allelic diversity and domestication patterns in wild chickpea. Sci Rep 5: 12468. Bastien M, Sonah H, Belzile F (2014). Genome wide association mapping of Sclerotinia sclerotiorum resistance in soybean with a genotyping-by-sequencing approach. Plant Genome 7: 1-13.
  2. Becerra VV, Paredes MC, Debouck D (2011). Genetic relationships of common bean (Phaseolus vulgaris L.) race Chile with wild Andean and Mesoamerican germplasm. Chil J Ag Res 71: 3-15.
  3. Benjak A, Ercisli S, Vokurka A, Maletic E, Pejic I (2005). Genetic relationships among grapevine cultivars native to Croatia, Greece and Turkey. Vitis 44: 73-77.
  4. Bitocchi E, Nanni L, Bellucci E, Rossi M, Giardini A, Zeuli PS, Logozzo G, Stougaard J, McClean P, Attene G et al. (2012). Mesoamerican origin of the common bean (Phaseolus vulgaris L.) is revealed by sequence data. P Natl Acad Sci USA 109: 788- 796. Blair M, Diaz LM, Buendia HF, Duque MC (2009). Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.). Theor Appl Genet 119: 955-972.
  5. Blair MW, Cortés AJ, Penmetsa RV, Farmer A, Carrasquilla-Garcia N, Cook DR (2013). A high-throughput SNP marker system for parental polymorphism screening, and diversity analysis in common bean (Phaseolus vulgaris L.). Theor Appl Genet 126: 535-548.
  6. Blair MW, Soler A, Cortés AJ (2012). Diversification and population structure in common beans (Phaseolus vulgaris L.). PLoS One 7: e49488. Bolibok-Brągoszewska H, Heller-Uszyńska K, Wenzl P, Uszyński G, Kilian A, Rakoczy-Trojanowska M (2009). DArT markers for the rye genome - genetic diversity and mapping. BMC Genomics 10: 578. Burle ML, Fonseca JR, Kami JA, Gepts P (2010). Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity. Theor Appl Genet 121: 801-813.
  7. Cabral PDS, Soares TCB, Lima ABP, de Miranda FD, Souza FB, Gonçalves LSA (2011). Genetic diversity in local and commercial dry bean (Phaseolus vulgaris) accessions based on microsatellite markers. Genet Mol Res 10: 140-149.
  8. Corrado G, Piffanelli P, Caramante M, Coppola M, Rao R (2013). SNP genotyping reveals genetic diversity between cultivated landraces and contemporary varieties of tomato. BMC Genomics 14: 835. Cortés AJ, Chavarro MC, Blair MW (2