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Journals / Trakya University Journal of Natural Sciences / 2021 / Cilt: 22 - Sayı: 1

DISTINCTIVE SIRE1 RETROTRANSPOSON PATTERNS ON BARLEY CHROMOSOMES?

Elif KARLIK
Nermin GÖZÜKIRMIZI
Pages
9–15
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results may provide to uncover the organization of SIRE retrotransposon pattern in barley genome.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:SIRE1 is an active and relatively high copy-number retroelement belongs to the Tyl/Copia long terminal repeat (LTR) retrotransposon superfamily.
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Key Findings & Data:Distinctive SIRE1 elements (ENV and GAG) distributions in barley genome were observed by using fluorescent in situ hybridization (FISH).
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Conclusion & Impact:These results may provide to uncover the organization of SIRE retrotransposon pattern in barley genome.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

SIRE1 is an active and relatively high copy-number retroelement belongs to the Tyl/Copia long terminal repeat (LTR) retrotransposon superfamily. Distinctive SIRE1 elements (ENV and GAG) distributions in barley genome were observed by using fluorescent in situ hybridization (FISH). We performed PCR to obtain tetramethylrhodamine-dUTP (TRITC)-labelled probes. Localizations of SIRE1 ENV and GAG domains were demonstrated under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed the distributions of SIRE1 elements ENV and GAG in barley genome. These results may provide to uncover the organization of SIRE retrotransposon pattern in barley genome.

Özet

SIRE1, Tyl/Copia Uzun Uç Tekrarlı (Long Terminal Repeats- LTR) retrotranspozon üst ailesine ait olan aktif, nispeten yüksek kopyalı bir retroementtir. Arpa genomundaki ayırt edici SIRE1 elementlerinin (ENV ve GAG) dağılımları floresan in situ hibridizasyonu (FISH) kullanılarak gözlemlendi. Tetramethylrhodamine-dUTP (TRITC)-işaretli probların elde edilmesinde PCR gerçekleştirildi. SIRE1 ENV ve GAG domainlerinin yerleşimleri, Hordeum vulgare L. cv. Hasat kök preparatlarında konfokal mikroskobu altında gösterildi. Sonuçlarımız, arpa genomundaki SIRE1 elementlerinin ENV ve GAG dağılımlarını göstermektedir. Bu sonuçlar, SIRE1 elementlerinin arpa genomunun organizasyonunun ortaya çıkarılmasına katkı sağlayacaktır.

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Cite This Article

KARLIK, E., GÖZÜKIRMIZI, N. (2022). DISTINCTIVE SIRE1 RETROTRANSPOSON PATTERNS ON BARLEY CHROMOSOMES?. Trakya University Journal of Natural Sciences, Cilt: 22 - Sayı: 1, 9–15. https://ajindex.com/articles/598544

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