Journals / Tarım Bilimleri Dergisi / 2019 / Cilt: 25 - Sayı: 4

Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape

Pages
459–466
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In the light of these results, isolates can be used for multiple hydrolytic enzyme production in industry.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Black aspergilli newly isolated from local grape and date were investigated for production of hydrolytic enzymes including cellulase, tannase and pectinase.
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Key Findings & Data:Enzymatic activity was determined in the culture filtrate of liquid medium.
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Conclusion & Impact:In the light of these results, isolates can be used for multiple hydrolytic enzyme production in industry.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Black aspergilli newly isolated from local grape and date were investigated for production of hydrolytic enzymes including cellulase, tannase and pectinase. Isolates were morphologically and molecularly identified as Aspergillus niger, Aspergillus tubingensis, Aspergillus japonicus and Aspergillus aculeatus. Isolates were screened for enzyme production ability on solid and in liquid media. Enzymatic activity was determined in the culture filtrate of liquid medium. A total of six isolates were found to produce multiple hydrolytic enzymes. The highest activity of cellulase was produced by A. japonicus ZGM4 and A. aculeatus ZGM6 as 40 and 35 U g-1 dry biomass, respectively. All the isolates exhibited high level of tannase activity in the range of 150-343 U g-1 dry biomass after 24 h of incubation. A. tubingensis ZGM5 andA. aculeatus ZGM6 were found to produce the highest pectinase activity at a level of 130 and 117 U g-1 dry biomass, respectively. In the light of these results, isolates can be used for multiple hydrolytic enzyme production in industry.

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Cite This Article

GÜLER, F. K., GULSUNOGLU, Z., AKYILMAZ, M. K., RAES, K. (2022). Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape. Tarım Bilimleri Dergisi, Cilt: 25 - Sayı: 4, 459–466. https://ajindex.com/articles/594803

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References (10)

  1. Ang S K, Shaza E M, Adibah Y, Suraini A A & Madihah M S (2013). Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation. Process Biochemistry 48(9): 1293-1302
  2. Banerjee D, Mondal K & Pati B (2007). Tannase production by Aspergillus aculeatus DBF9 through solid state fermentation. Acta Microbiologica et Immunologica Hungarica 54(2): 159-166
  3. Botella C, De Ory I, Webb C, Cantero D & Blandino A (2005). Hydrolytic enzyme production by Aspergillus awamori on grape pomace. Biochemical Engineering Journal 26(2): 100-106
  4. Bradoo S, Gupta R & Saxena R K (1996). Screening of extracellular tannase-producing fungi: Development of a rapid and simple plate assay. Journal of General and Applied Microbiology 42: 325-329
  5. Buenrostro-Figueroa J J, Velázquez M, Flores-Ortega O, Ascacio-Valdés J A, Huerta-Ochoa S, Aguilar C N & Prado-Barragán L A (2017). Solid state fermentation of fig (Ficus carica L.) by-products using fungi to obtain phenolic compounds with antioxidant activity and qualitative evaluation of phenolics obtained. Process Biochemistry 62: 16-23
  6. Debing J, Peijun L, Stagnitti F, Xianzhe X & Ling L (2006). Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment. Ecotoxicology and Environmental Safety 64(2): 244-250
  7. Dulf F V, Vodnar D C & Socaciu C (2016). Effects of solid-state fermentation with two filamentous fungi on the total phenolic contents, flavonoids, antioxidant activities and lipid fractions of plum fruit (Prunus domestica L.) by-products. Food Chemistry 209: 27- 36 Gautam S P, Bundela P S, Pandey A K, Khan J, Awasthi M K & Sarsaiya S (2011). Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotechnology Research International 2011: 1-8
  8. GenBank (2016). National Center for Biotechnology Information (NCBI), US National Library of Medicine. Retrieved in June, 25, 2016 from http:// www.ncbi.nlm.nih.gov/ Heerd D, Yegin S, Tari C & Fernandez-Lahore M (2012). Pectinase enzyme-complex production by Aspergillus spp. in solid-state fermentation: A comparative study. Food and Bioproducts Processing 90(2): 102-110
  9. Imran M, Anwar Z, Irshad M, Javid A, Hussain A & Ali S (2017). Optimization of cellulase production from a novel strain of Aspergillus tubingensis IMMIS2 through response surface methodology. Biocatalysis and Agricultural Biotechnology 12: 191-198
  10. Jayani R S, Saxena S & Gu