Journals / Su Ürünleri Dergisi / 2005 / Cilt: 22 - Sayı: 1-2

Vegatative growth characteristics of Haematococcus pluvialis Flotow at different light intensities

Haematococcus pluvialis Flatow (Chlorophyceae)' un farklı ışık şiddetlerinde vejetatif büyüme özellikleri

Pages
21–24
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, astaxanthin accumulation was triggered and cell number was decreased above the illuminations of 200 $mu$ mol photon $m^{-2}sn^{-1}$ (12.73 x $10^4$ , 16.10 x $10^4$ ,19.73 x $10^4$ ,15.90 x $10^4$ and 12,89 x $10^4$ cells $ml^{-1}$ for 50,100,200,400 and 600 $mu$ mol photon $m^{-2}sn^{-1}$ , respectively).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Five different light intensities of 50,100, 200, 400 and 600 $mu$ mol photon $m^{-2}sn^{-1}$ were applied on the vegetative cultures of Haematococcus pluvialis to determine the optimal light intensity, and the growth performance.
📊
Key Findings & Data:While dry weight and total carotenoid amounts increased in parallel to the light intensities, total chlorophyll amount increased up to the irradiance of 200 $mu$ mol photon $m^{-2}sn^{-1}$ (2.21 mg $L^{-1}$ ), and no change was observed in the higher illuminations (mean value of 2.35 mg $L^{-1}$ ).
💡
Conclusion & Impact:In conclusion, astaxanthin accumulation was triggered and cell number was decreased above the illuminations of 200 $mu$ mol photon $m^{-2}sn^{-1}$ (12.73 x $10^4$ , 16.10 x $10^4$ ,19.73 x $10^4$ ,15.90 x $10^4$ and 12,89 x $10^4$ cells $ml^{-1}$ for 50,100,200,400 and 600 $mu$ mol photon $m^{-2}sn^{-1}$ , respectively).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Five different light intensities of 50,100, 200, 400 and 600 $mu$ mol photon $m^{-2}sn^{-1}$ were applied on the vegetative cultures of Haematococcus pluvialis to determine the optimal light intensity, and the growth performance. While dry weight and total carotenoid amounts increased in parallel to the light intensities, total chlorophyll amount increased up to the irradiance of 200 $mu$ mol photon $m^{-2}sn^{-1}$ (2.21 mg $L^{-1}$ ), and no change was observed in the higher illuminations (mean value of 2.35 mg $L^{-1}$ ). Interestingly, cell count increased in all the groups from the mean value of 3.45 x $10^4$ cells ml1 on the 15th hour to 5.66 x $10^4$ cells $ml^{-1}$ on the 18th hour, which coincided with the dark period. In conclusion, astaxanthin accumulation was triggered and cell number was decreased above the illuminations of 200 $mu$ mol photon $m^{-2}sn^{-1}$ (12.73 x $10^4$ , 16.10 x $10^4$ ,19.73 x $10^4$ ,15.90 x $10^4$ and 12,89 x $10^4$ cells $ml^{-1}$ for 50,100,200,400 and 600 $mu$ mol photon $m^{-2}sn^{-1}$ , respectively). In the experiment applied five different illuminations, optimal light intensity range for the vegetative stage cultivation of the cells was found to be 50 - 200 $mu$ mol photon $m^{-2}sn^{-1}$ and the best growth was in the light intensity of 200 $mu$ mol photon $m^{-2}sn^{-1}$ .

Özet

Haematococcus pluvialis'm vejetatif kültürlerinde, optimal ışık şiddeti aralığı ve büyüme performanslarının tespiti için 50,100, 200, 400 ve 600 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik beş farklı ışık şiddeti uygulandı. Deneme gruplarında kuru ağırlık ve toplam karaten miktarları artan ışık şiddetleri ile doğru orantılı olarak artarken, toplam klorofil miktarı 200 $mu$ mol foton $m^{-2}sn^{-1}$'lik aydınlatmaya kadar arttı (2.21 mg $L^{-1}$ ) ve bunun üzerindeki aydınlatma şiddetlerinde herhangi bir değişim görülmedi (ortalama 2.35 mg $L^{-1}$ ). Hücre sayısı tüm gruplarda karanlık periyoda denk gelen 15. saatte ortalama 3.45 x $10^4$ hücre $ml^{-1}$'den 18. saatte 5.66 x $10^4$ hücre $ml^{-1}$ 'ye arttı. Sonuç olarak, 200 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik ışık şiddetinin üzerindeki aydınlatmalarda astaksantin birikiminin tetiklendiği ve hücre sayısının azaldığı görüldü (50,100, 200, 400 ve 600 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik ışık şiddetleri için sırasıyla 12.73 x $10^4$ ,16.10 x $10^4$ , 19.73 x $10^4$ , 15.90 x $10^4$ ve 12,89 x $10^4$ hücre $ml^{-1}$ ). Beş farklı ışık şiddetinin uygulandığı denemede hücrelerin vejetatif safhada kültür edilebilmesi için optimal ışık şiddeti aralığı 50 - 200 $mu$ mol foton $m^{-2}sn^{-1}$ olarak bulundu ve en iyi büyüme 200 $mu$ mol foton $m^{-2}sn^{-1}$ 'lik ışık şiddetinde gerçekleşti.

🔖

Cite This Article

GÖKPINAR, Ş., GÖKSAN, T. (2022). Vegatative growth characteristics of Haematococcus pluvialis Flotow at different light intensities. Su Ürünleri Dergisi, Cilt: 22 - Sayı: 1-2, 21–24. https://ajindex.com/articles/593687

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (17)

  1. Ako, H., C.S. Tamaru, 1999. Are feeds for food fish practical for aquarium fish? Intl. Aqua Feeds. 2:30-36.
  2. Belarbi E.H., Molina E., Christi Y.A., 2000. Process for high yield and scaleable recovery of high purity eicosapentaenoic acid esters from 1 microalgae and fish oil. Enzyme Microbiol Technol., 26:516-29.
  3. Boussiba, S., A. Vonshak, 1991. Astaxanthin accumulation in the green alga H. pluvialis, Plant Cell Physiol., 32:1077-1082.
  4. Boussiba, S., 2000. Carotenogenesis in the green alga Haematococcus pluvialis: cellular physiology and stress response, Physiol. Plantarum, 108:111-117.
  5. Davis, B.H., 1985. Carotenoid metabolism in animals: a biochemist's view, Pure Appl. Chem., 57:679-684.
  6. Elliot, A.M., 1934. Morphology and life history of Haematococcus pluvialis, Archiv. Protistekunde, 82:250-272.
  7. Gökpınar, Ş., 1980. Observations on the culture of a marine diatom Phaeodactylum tricomutum (in Turkish), TÜBİTAK VII. Bilim Kong., 827- 840, Kuşadası, İZMİR. Gökpınar, Ş, 1982. Observations on the culture of a marine diatom Phaeodactylum tricomutum in different nutrient and salinity concentrations, E.Ü. Fac. of S.Ü., Jour.Ser.B., 6:77-86.
  8. Göksan, T., G. Torzillo, M. Sergejevova, M. Sekerkova, M. Verbovikova, Ş. Gökpınar, J. Kopecky, J. Masojidek, 2003. Microalgae cultivation in a new type of photobioreactor-preliminary studies, (in Turkish), XII. Ulusal Su Ürünleri Sempozyumu, Fırat Üniversitesi,
  9. Su Ürünleri Fakültesi, Elazığ, Eylül 2003 (in press). Guerin, M., M.E. Huntley, M. Olaizola, 2003. Haematococcus astaxanthin: applications for human health and nutrition, Trends in Biotecgnology, 21: 210-216.
  10. Inborr, J., 1998.
  11. Haematococcus, The poultry pigmentor, Feed Mix., 6:31-34.
  12. Johnson, E.A., G.H. An, 1991.
  13. Astaxanthin from microbial sources, Crit. Rev. Biotechnol., 11:297-326.
  14. Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: pigments of photosynthetic membranes, Methods Enzymol. 148:349-382.
  15. Masojidek, J., G. Torzillo, M. Koblizek, J. Kopecky, L. Nidiaci, J. Komenda, A. Lukavska, A. Sacchi, 2000. Changes in chlorophyll fluorescence quenching and pigment composition in the green alga Chlorococcum sp. grown under nitrogen deficiency and salinity stress, J. Appl. Phycol., 12: 417-426.
  16. Rippka, R., J.B. Deruelles, M. Herdman, B. Waterbury, R.Y. Stanier, 1979. Generic assignments, strain history and properties of pure cultures of Cyanobacteria, J.Gen.Microbiol., 111:1-61.
  17. Terao, J., 1989. Antioxidant activity of beta-carotene related carotenoids in solution, Lipids