Journals / Su Ürünleri Dergisi / 2020 / Cilt: 37 - Sayı: 2

Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile

Farklı protein kaynaklarının turna, Esox lucius Linneaus 1758, jüvenillerinin proteaz aktivitesi üzerine etkisi

Pages
135–138
Publication Date
2022-10-17
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Additionally, chicken meal again has the highest effect on juveniles with the ratio 89.27 %.As a result, the increase of proteolytic activity was notably increased in 7-14-21 DAH, however, it is concluded that feeding larvae and juveniles with live preyis still suggested to get better result for such a carnivorous species culture.Keywords: Northern pike, protease activity, protein sources, inhibition effect

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of the study is to determine the inhibitory effect of different protein sources on protease activity of Northern pike, E. lucius, during larvalontogeny.
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Key Findings & Data:For this purpose, Northern pike were fed from yolk sac absorption until 21 days after hatching (DAH).
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Conclusion & Impact:Additionally, chicken meal again has the highest effect on juveniles with the ratio 89.27 %.As a result, the increase of proteolytic activity was notably increased in 7-14-21 DAH, however, it is concluded that feeding larvae and juveniles with live preyis still suggested to get better result for such a carnivorous species culture.Keywords: Northern pike, protease activity, protein sources, inhibition effect
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of the study is to determine the inhibitory effect of different protein sources on protease activity of Northern pike, E. lucius, during larvalontogeny. For this purpose, Northern pike were fed from yolk sac absorption until 21 days after hatching (DAH). At that point, larvae were sampled on 7, 14and 21 DAH days and the activity of enzyme was analyzed in vitro. In the study, two different fish meal, chicken meal, krill meal, corn gluten, soybean proteinconcentrate, soybean meal and dried distillers grains with solubles were tested. Fish meal-I showed the lowest effect (7.53 %) on 7 DAH larvae. Moreover,chicken meal has the highest inhibitory effect on the proteases in the first week (68.27%). In the following period (DAH 14), although the inhibition ratiodramatically increased in all ingredients, fish meal-I has still the lowest effect on proteolytic activity (55.66%). In the same period, the highest effect wasobtained from krill (82.28 %) and chicken meals (86.73 %), respectively. Then, there was no statistical difference between fish meal-I, fish meal-II and corngluten in the 21 DAH and relatively lower than the others (p>0.05). Additionally, chicken meal again has the highest effect on juveniles with the ratio 89.27 %.As a result, the increase of proteolytic activity was notably increased in 7-14-21 DAH, however, it is concluded that feeding larvae and juveniles with live preyis still suggested to get better result for such a carnivorous species culture.Keywords: Northern pike, protease activity, protein sources, inhibition effect

Özet

Bu çalışmanın amacı, farklı protein kaynaklarının turna balığının larval gelişimi sırasında sindirim enzimlerinden proteaz enzimlerini inhibe edici etkisinin tespit edilmesidir. Bu amaçla, Turna balığı, E. lucius, larvaları yumurtadan çıkıştan itibaren 21. günün sonuna kadar beslenmişlerdir. Bu noktada, besleme yapılan larvalardan 7, 14 ve 21. günlerde örnekler alınarak in vitro enzim aktiviteleri tespit edilmiştir. Çalışmada, protein kaynağı olarak iki farklı balık unu, tavuk unu, krill unu, mısır glüteni, soya protein konsantresi, soya unu ve damıtılmış tahıl test edilmiştir. Yedinci günde, en düşük etki balık unu-I grubunda tespit edilmiştir (% 7,53). Ayrıca, tavuk unu ilk hafta en yüksek durdurucu etkiyi göstermiştir (% 68,27). Bunu takip eden dönemde (14. gün), tüm hammaddelerin durdurucu etkisi dramatik bir şekilde artmış olsa da, proteolitik aktivite üzerindeki en düşük etki yine balık unu-I grubunda görülmüştür (% 55,66). Aynı dönem içerisinde, en yüksek etki krill ve tavuk ununda tespit edilmiştir (sırasıyla, %82,28 ve 86,73). Ardından, 21. günde ise balık unu-I, balık unu-II ve mısır gluteni arasında herhangi bir istatistiki fark bulunmamıştır (p>0.05). Bunun yanında, tavuk unu bu dönemde de enzim aktivitesini durdurucu en yüksek etkiye sahip olmuştur (% 89,27). Sonuç olarak, 7- 14 ve 21. günlerde proteolitik aktivitenin belirgin artış göstermesi ile birlikte, bu türden karnivor balıkların larva ve juvenil boylarının beslenmesi sırasında canlı yemlerin kullanılmasının daha başarılı gelişim sonuçlarını ortaya koyacağı düşünülmektedir.

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Cite This Article

0000-0003-3535-2236, 0., Gökçek, K., Szabó, T. (2022). Effect of different protein sources on protease activity of northern pike, Esox lucius Linneaus 1758, juvenile. Su Ürünleri Dergisi, Cilt: 37 - Sayı: 2, 135–138. https://ajindex.com/articles/593528

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