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Journals / Selçuk Tıp Dergisi / 2021 / Cilt: 37 - Sayı: 2

Vascular Effects of Ginkgo Biloba Extract (EGb-761) on Isolated Human Umbilical Artery Contraction Responses

Ginkgo Biloba Ekstresinin (EGb-761) İzole İnsan Umbilikal Arteri Kasılma Yanıtları Üzerine V asküler Etkileri

Ipek Duman
Cigdem Gokbas
Pages
158–165
Publication Date
2022-10-17
DOI
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Results: Cumulative EGb-761 generated concentration-dependent relaxation in 5-HT induced contraction (p

📌 3 Key Takeaways

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Objective & Hypothesis:This in vitro study assesses the vascular effects of EGb-761 on the isolated human umbilical artery and the role of nitric oxide (NO) and prostaglandins in these effects.
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Key Findings & Data:Procedures were conducted in different groups of strips (n=9); 1) Effect of cumulative EGb-761 on the basal tonus of the artery. 2) The relaxant effect of cumulative EGb-761 on contraction elicited by 10-6 M 5-HT. 3) Effect of L-NAME incubation on the relaxant effect of cumulative EGb-761 on contraction elicited by 5-HT. 4) Effect of indomethacin incubation on the relaxant effect of cumulative EGb-761 on contraction elicited by 5-HT. 5) Combined effect of L-NAME and indomethacin incubation on the relaxant effect of cumulative EGb-761 on contraction elicited by 5-HT.
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Conclusion & Impact:Results: Cumulative EGb-761 generated concentration-dependent relaxation in 5-HT induced contraction (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This in vitro study assesses the vascular effects of EGb-761 on the isolated human umbilical artery and the role of nitric oxide (NO) and prostaglandins in these effects. Materials and Methods: Human umbilical artery strips were suspended in organ baths containing KrebsHenseleit solution. Isometric vasoactive changes to the applied agents were recorded. Procedures were conducted in different groups of strips (n=9); 1) Effect of cumulative EGb-761 on the basal tonus of the artery. 2) The relaxant effect of cumulative EGb-761 on contraction elicited by 10-6 M 5-HT. 3) Effect of L-NAME incubation on the relaxant effect of cumulative EGb-761 on contraction elicited by 5-HT. 4) Effect of indomethacin incubation on the relaxant effect of cumulative EGb-761 on contraction elicited by 5-HT. 5) Combined effect of L-NAME and indomethacin incubation on the relaxant effect of cumulative EGb-761 on contraction elicited by 5-HT. Results: Cumulative EGb-761 generated concentration-dependent relaxation in 5-HT induced contraction (p

Özet

Bu in vitro çalışma, EGb-761'in izole insan umbilikal arter üzerindeki vasküler etkilerini ve bu etkilerde nitrik oksit (NO) ve prostaglandinlerin rolünü değerlendirmeyi amaçlamaktadır. Gereçler ve Yöntem: Umbilikal arter şeritleri, Krebs-Henseleit solüsyonu içeren organ banyolarında asıldı. Uygulanan ajanların oluşturduğu izometrik vazoaktif değişiklikler kaydedildi. Farklı doku gruplarında (n=9) şu deneysel prosedürler yapıldı; 1) Kümülatif uygulanan EGb-761'in umbilikal arter şeritlerinin bazal tonusu üzerindeki etkisi. 2) Kümülatif EGb-761'in 10-6 M 5-HT ile arter şeritlerinde oluşturulan kasılma yanıtı üzerindeki gevşetici etkisi. 3) Kümülatif EGb-761'in L-NAME ile inkübasyon sonrası 5-HT ile arter şeritlerinde oluşturulan kasılma yanıtı üzerindeki gevşetici etkisi 4) Kümülatif EGb-761'in indometazin ile inkübasyon sonrası 5-HT ile arter şeritlerinde oluşturulan kasılma yanıtı üzerindeki gevşetici etkisi ve 5) Kümülatif EGb-761'in L-NAME ve indometazin ile inkübasyonu sonrası 5-HT ile arter şeritlerinde oluşturulan kasılma yanıtı üzerindeki gevşetici etkisi. Bulgular: Kümülatif EGb-761, 5-HT kaynaklı kasılma cevaplarında konsantrasyona bağlı gevşeme oluşturdu (p

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Cite This Article

Duman, I., Gokbas, C. (2022). Vascular Effects of Ginkgo Biloba Extract (EGb-761) on Isolated Human Umbilical Artery Contraction Responses. Selçuk Tıp Dergisi, Cilt: 37 - Sayı: 2, 158–165. https://ajindex.com/articles/591798

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References (11)

  1. Pinto MDS, Kwon YI, Apostolidis E, et al. Potential of Ginkgo biloba L. leaves in the management of hyperglycemia and hypertension using in vitro models. Bioresour Technol 2009;100(24):6599-609. Bilia AR. Ginkgo biloba L. Fitoterapia 2002;73(3):276-9.
  2. Singh SK, Srivastav S, Castellani RJ, et al. Neuroprotective and antioxidant effect of Ginkgo biloba extract against AD and other neurological disorders. Neurotherapeutics 2019;16(3):666-74.
  3. Zhou, W, Chai H. Lin PH, et al. Clinical use and molecular mechanisms of action of extract of Ginkgo biloba leaves in cardiovascular diseases. Cardiovasc Drug Rev 2004;22(4):309-19.
  4. Chan PC, Xia QS, Fu PP. Ginkgo biloba leave extract: Biological, medicinal, and toxicological effects. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2007;25(3):211- 44. Tian J, Liu Y, Chen K. Ginkgo biloba extract in vascular protection: molecular mechanisms and clinical applications, Curr Vasc Pharmacol 2017;15:532-48. EGb 761: ginkgo biloba extract, Ginkor. Drugs R D 2003;4(3):188-93.
  5. Delaflotte S, Auguet M, DeFeudis F, et al. Endotheliumdependent relaxations of rabbit isolated aorta produced by carbachol and by Ginkgo biloba extract. Biomed Biochim Acta 1984;43(8-9):S212-6. Kubota Y, Tanaka N, Umegaki K, et al. Ginkgo biloba extract-induced relaxation of rat aorta is associated with increase in endothelial intracellular calcium level. Life Sci 2001;69(20):2327-36.
  6. Chen X, Salwinski S, Lee TF. Extracts of Ginkgo biloba and ginsenosides exert cerebral vasorelaxation via a nitric oxide pathway. Clin Exp Pharmacol Physiol 1997;24(12):958-9.
  7. Chen X, Liu L, Li Z. Cardiovascular protective effects and NOmediated cerebrovasorelaxant effects of extract of Ginkgo biloba leaves. Zhonghua Yi Xue Za Zhi 1998;78(9):692-5.
  8. Lorigo M, Mariana M, Feiteiro J, et al. How is the human umbilical artery regulated? J Obstet Gynaecol Res 2018;44(7):1193-201.
  9. Dagtekin E, Sahin AS. Effects of leptin on hydrogen peroxide induced contractions in isolated human umbilical artery. Selcuk Med J 2018;34(4):160-4.
  10. Coyoy-Salgado A, Segura-Uribe JJ, Guerra-Araiza C, et al. The importance of natural antioxidants in the treatment of spinal cord injury in animal models: An overview. Oxid Med Cell Longev 2019;2019:3642491. Nishida S, Satoh H. Mechanisms for the vasodilations induced by Ginkgo biloba extract and its main constituent, bilobalide, in rat aorta, Life Sci 2003;72(23):2659-67.
  11. Nishida S, Satoh H. Comparative vasodilating actions among terpenoids and flavonoids