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Journals / Selcuk Dental Journal / 2020 / Cilt: 7 - Sayı: 1

Ozone improves autogenous graft healing in experimental diabetes mellitus: A morphometric and immunohistochemical study

Ozonun deneysel diabetes mellitustta otojen greft iyileşmesini artırması: Morfometrik ve immünhistokimyasal çalışma

Hatice ÖZER
Aysan LEKTEMUR ALPAN
Hülya TOKER
Pages
44–53
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Osteocalcin immune positivity inAG groups was higher than those of the Control groups.Conclusion: Within the limitations of this study, gaseous ozoneapplication decreased osteoclast number and increasedosteoblast number and bone regeneration, especially, in earlystages of bone regeneration in diabetic rats

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Diabetes mellitus (DM) is a metabolic disorder whichplays crucial role in the pathogenesis of periodontitis.
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Key Findings & Data:Study groups: 1-Empty defect(Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Emptydefect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application (AG+Ozone, n=14) group.
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Conclusion & Impact:Osteocalcin immune positivity inAG groups was higher than those of the Control groups.Conclusion: Within the limitations of this study, gaseous ozoneapplication decreased osteoclast number and increasedosteoblast number and bone regeneration, especially, in earlystages of bone regeneration in diabetic rats
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Diabetes mellitus (DM) is a metabolic disorder whichplays crucial role in the pathogenesis of periodontitis. Ozone haveseveral actions such as antimicrobial, immunostimulating,antihypoxic effects, and activates antioxidant enzymes andangiogenesis. The aim of this study is to investigate the effect ofgaseous ozone on bone healing in diabetic rat calvarial defectstreated with autogenous bone graft, morphometrically andimmunohistochemically.Material and Methods: Diabetes was induced and critical sizedefects were created on rats. Study groups: 1-Empty defect(Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Emptydefect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application (AG+Ozone, n=14) group. Gaseousozone was applied on the operation day and the following 2 weeksdaily (140ppm @ 2L/d, 2.24 mg). Total bone area was measured.Osteocalcin and Bone morphogenic protein-2 protein expressionswere evaluated.Results: Control and Control+Ozone groups had no osteoclastand residuel lacunae during the study . Osteoblasts in AG+Ozonegroup were higher than AG group at 4th week (p>0.05).AG+Ozone group had more total bone area than AG group at 4thweek. AG+Ozone group revealed more BMP-2 immune positivitycompared to the other groups. Osteocalcin immune positivity inAG groups was higher than those of the Control groups.Conclusion: Within the limitations of this study, gaseous ozoneapplication decreased osteoclast number and increasedosteoblast number and bone regeneration, especially, in earlystages of bone regeneration in diabetic rats

Özet

Amaç: Diabetes mellitus (DM), periodontitis patogenezinde önemli rol oynayan bir metabolik bozukluktur. Ozonun antimikrobiyal, immünostimülasyon, antihipoksik etkileri gibi çeşitli etkileri vardır ve antioksidan enzimleri ve anjiyogenezi aktive eder. Bu çalışmanın amacı otojen kemik grefti ile tedavi edilen diyabetik sıçan kalvaryal defektlerinde uygulanan ozonunun kemik iyileşmesi üzerine etkisini morfometrik ve immünhistokimyasal olarak incelemektir. Gereç ve Yöntemler: Sıçanlarda diyabet indüklendi ve kalvaryal defektler oluşturuldu. Çalışma grupları: 1-Boş defekt (Kontrol, n = 14) grubu, 2-Otogreft (AG, n = 14) grubu, 3-Boş defekt + ozon tedavisi (Kontrol + Ozon, n = 14) grubu, 4-Otogreft + Ozon uygulaması (AG + Ozon, n = 14) grubu. Operasyon gününde ve sonraki 2 haftada gaz ozon (140ppm2L / d, 2.24 mg) kalvaryal bölgeye uygulandı. Toplam kemik alanı ölçüldü. Osteokalsin ve Kemik morfojenik protein-2 protein ekspresyonları değerlendirildi. Bulgular: Kontrol ve Kontrol + Ozon gruplarında çalışma süresince osteoklast ve rezidüel lakün gözlenmedi. AG + Ozon grubunda osteoblastlar, 4. haftada AG grubundan daha fazla gözlendi (p> 0.05). AG + Ozon grubunun 4. haftada AG grubundan daha fazla toplam kemik alanı vardı. AG + Ozon grubu diğer gruplara göre daha fazla BMP-2 immün pozitifliği saptadı. AG gruplarında osteokalsin immün pozitifliği, kontrol gruplarından daha yüksekti. Sonuç: Bu çalışmanın kısıtlılıkları dahilinde, gaz ozon uygulaması, özellikle diyabetik sıçanlarda kemik rejenerasyonunun erken aşamalarında osteoklast sayısını azaltarak ve osteoblast sayısını artırarak kemik rejenerasyonunu artırmıştır.

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Cite This Article

ÖZER, H., ALPAN, A. L., TOKER, H. (2022). Ozone improves autogenous graft healing in experimental diabetes mellitus: A morphometric and immunohistochemical study. Selcuk Dental Journal, Cilt: 7 - Sayı: 1, 44–53. https://ajindex.com/articles/591432

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