AI Summary & Key Takeaways
AI InsightHowever, SOCS3 promoter methylation level was significantly higher for patients having locally advanced PCa compared to those having localizedPCa (p
📌 3 Key Takeaways
Abstract
Objective: The aim of this study was to investigate the promoter methylation status of Rasassociated domain family 1A (RASSF1A), O-6-methylguanine-DNA methyltransferase (MGMT),Phosphatase with tensin homology (PTEN) and Suppressor of cytokine signaling 3 (SOCS3) tumor suppressor genes and evaluate the clinical utility of these genes as noninvasive, bloodbased epigenetic biomarkers for the diagnosis of Prostate Cancer (PCa).Method: A total of 41 consecutive patients and 10 healthy control groups were enrolled in thestudy. Pyrosequencing was performed to analyze the methylation levels of the promoter regionsof the four tumor suppressor genes in patients compared to healthy controls.Results: The promoter methylation levels of RASSF1A, MGMT, PTEN and SOCS3 did not differbetween the patient and control groups. However, SOCS3 promoter methylation level was significantly higher for patients having locally advanced PCa compared to those having localizedPCa (p
Özet
Amaç: Bu çalışmanın amacı tümör baskılayıcı genler Ras-associated domain family 1A (RASSF1A), O-6-methylguanine-DNA methyltransferase (MGMT), Phosphatase with tensin homology (PTEN) ve Suppressor of cytokine signaling 3 (SOCS3)’in promotör metilasyonlarını araştırmak ve bu genlerin Prostat Kanseri (PK) tanısı için noninvaziv, kan temelli epigenetik biyobelirteçler olarak klinik yararını değerlendirmekti. Yöntem: 41 hasta ve 10 sağlıklı kontrol grubu çalışmaya kaydedildi. Dört tümör baskılayıcı genin promotör bölgelerinin metilasyon düzeylerini hastalarda sağlıklı kontrol grubuna kıyasla analiz etmek için pirodizileme gerçekleştirildi. Bulgular: RASSF1A, MGMT, PTEN ve SOCS3 promotör metilasyon düzeyleri hasta ve konrol grubu arasında farklı değildi. Ancak SOCS3 promotör metilasyon düzeyi lokalize PK’lı hastalara kıyasla lokal olarak ilerlemiş PK’lı hastalarda önemli şekilde daha yüksekti (p