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Journals / Marine Science and Technology Bulletin / 2020 / Cilt: 9 - Sayı: 2

Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity

Mahinur KIRICI
Muhammed ATAMANALP
Muammer KIRICI
Şükrü BEYDEMİR
Pages
92–101
Publication Date
2022-10-19
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag+.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the in vitro effects of some metal ions (Ag+, Cd2+, Cu2+, Fe2+, Ni2+ and Pb2+) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated.
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Key Findings & Data:For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively.
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Conclusion & Impact:Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag+.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, the in vitro effects of some metal ions (Ag+, Cd2+, Cu2+, Fe2+, Ni2+ and Pb2+) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated. For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively. In order to control the enzyme purification SDS-PAGE showed a single band for the enzyme. In addition, in vitro effects of metal ions on enzyme activity were researched. As a result, Ag+, Cd2+, Cu2+, Ni2+ and Pb2+ inhibited fish liver G6PD; Ag+, Cd2+, Fe2+, Ni2+ and Pb2+ inhibited fish gill G6PD. Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag+. Our results also demonstrate that these metals might be dangerous at low micromolar concentrations for fish G6PD enzyme.

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Cite This Article

KIRICI, M., ATAMANALP, M., KIRICI, M., BEYDEMİR, Ş. (2022). Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity. Marine Science and Technology Bulletin, Cilt: 9 - Sayı: 2, 92–101. https://ajindex.com/articles/578268

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