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Journals / Klinik Psikofarmakoloji Bülteni / 2001 / Cilt: 11 - Sayı: 2

In vitro effects of chlorpromazine and haloperidol on human erythrocyte glucose 6-phosphate dehydrogenase enzyme

Klorpromazin ve haloperidol'ün insan eritrosit glukoz 6-fosfat dehidrogenaz enzimi üzerine invitro etkileri

Mehmet Emin BÜYÜKOKUROĞLU
Zekiye Nur BANOĞLU
Sayit ALTIKAT
Fatma GÖÇER
Mehmet ÇİFTÇİ
Pages
101–105
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The results of this study suggested that chlorpromazine and haloperidol have significant inhibition effect on the activity of glucose 6-phosphate dehydrogenase enzyme in invitro

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Effects of chlorpromazine and haloperidol on erythrocyte glucose 6-phosphate dehydrogenase enzyme activity were investigated in invitro conditions.
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Key Findings & Data:Methods: Glucose 6-phosphate dehydrogenase was purified from human erythrocyte.
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Conclusion & Impact:Conclusion: The results of this study suggested that chlorpromazine and haloperidol have significant inhibition effect on the activity of glucose 6-phosphate dehydrogenase enzyme in invitro
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Effects of chlorpromazine and haloperidol on erythrocyte glucose 6-phosphate dehydrogenase enzyme activity were investigated in invitro conditions. Methods: Glucose 6-phosphate dehydrogenase was purified from human erythrocyte. Enzyme activity was determined according to the Beutler method by using a spectrophotometer at 340 nm. Results: The results of study showed that I50 values were 0,42 mM for chlorpromazine and 0,37 mM for haloperidol in invitro study. Conclusion: The results of this study suggested that chlorpromazine and haloperidol have significant inhibition effect on the activity of glucose 6-phosphate dehydrogenase enzyme in invitro

Özet

Amaç: Klorpromazin ve haloperidolün in vitro koşullarda insan eritrosit glukoz 6-fosfat dehidrogenaz enzimi üzerine etkileri belirlemek amacıyla bu çalışma yapıldı. Yöntem: Glukoz 6-fosfat dehidrogenaz enzimi insan eritrositlerinden saflaştırıldı. Enzim aktivitesinin tayini Beutler metoduna göre spektrofotometrik olarak 340nm 'de yapıldı. Bulgular: Çalışmanın sonuçlarına göre 150 değerleri klorpromazin için 0,42 mM, haloperidol için 0,37 mM oldu. Sonuç: Elde edilen veriler göstermektedir ki, klorpromazin ve haloperidol glukoz 6-fosfat dehidrogenaz enziminin aktivitesini in vitro olarak güçlü bir şekilde inhibe etmiştir.

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Cite This Article

BÜYÜKOKUROĞLU, M. E., BANOĞLU, Z. N., ALTIKAT, S., GÖÇER, F., ÇİFTÇİ, M. (2022). In vitro effects of chlorpromazine and haloperidol on human erythrocyte glucose 6-phosphate dehydrogenase enzyme. Klinik Psikofarmakoloji Bülteni, Cilt: 11 - Sayı: 2, 101–105. https://ajindex.com/articles/574533

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References (12)

  1. T elefoncu A, Telefoncu F. Glukoz-6-fosfat dehidrogenaz aktivitesine primaquine’nin etkisi. Tr J Medical Sci 1989
  2. Sodeman WA Jr, Sodeman TM. Sodeman’s Pathologic Physiology Mechanism of Disease, 1985. Çev: kurul, Fizyopatoloji cilt-2, Ankara:Türkiye Klinikleri Yayınevi, 1991:720-724. 3.K ayaalp SO. Rasyonel Tedavi Yönünden Tıbbi Farmakoloji. 9. Baskı. Ankara: Hacettepe-Taş Kitapçılık 2000:155-156. 4. Weksler BB, Moore A, Tepler J. Hematology. In: Andreoli TE, Carpenter CCJ, Plum F, Smith LH Jr eds. Cecil Essentials of Medicine. USA: WB Saunders Co., 1990:342-403. 5.Laur ence DR, Bennett PN, Brown MJ. Clinical Pharmacology, China: Churchil Livingstone 1997:77-120. 6.Cotr an RS, Kumar V, Collins TR. Pathological Basis of Disease. USA: W.B. Saunders Co. 1999:601-643. 7.T amer l, Ünal B, Aksoy K. Glukoz-6-fosfat dehidrogenaz enzim eksikliği gözlenen olgularda eritrosit zarı Na+- K+/Mg++ adenozin 5’-trifosfataz, eritrosit süperoksid dismutaz ve plazma malondialdehid düzeyleri. Ç.Ü. Tıp Fakültesi Dergisi 1998
  3. Beutler E. G6PD deficiency. Blood 1994
  4. Shr eve DS, Levy HR. On the molecular weight of human glucose-6-phosphate dehydrogenase. Biochem Biophys Res Com 1977
  5. Çiftçi M, Küfrevioğlu Öİ, Gündoğdu M, Özmen İ. Effects of some antibiotics on enzyme activity of glucose-6- phosphate dehydrogenase from human erythrocytes. Pharmacol Res 2000
  6. Ninfali P, Orsenigo T, Baor ciani L, Rapa SR.apid purification of glucose-6-phosphate dehydrogenase from mammal’s erythrocyte. Prep Biochem 1990
  7. Delgado C, Tejedor C, Luquue J. Partial purification of glucose-6-phosphate dehydrogenase from rat erythrocyte haemolysate by partitioning in aqueous two-phase system. J Chromatogr 1990
  8. Mor elli A, Benatti U, Gaetani GF, De Flora A. Biochemical mechanisms of glucose-6-phosphate dehydrogenase deficiency. Proc Natl Acad Sci 1978
  9. Laemmli DK. Clevage of structural proteins during in assembly of the head of bacteriophage T4. Nature 1970
  10. Beutler E. Red Cell Metabolism Manuel of Biochemical Methods. London: Academic Press 1971. 16.Nas r J. Glucose-6-phosphate dehydrogenase deficiency with psychosis. Arch Gen Psychiatry 1976
  11. Alevizos BH, Stefanis CN. Enzyme activity of G-6-PD, gamma-GT and Lysozyme in white cells of schizophrenics. Neuropsychobiology 1980
  12. Nor gaad T, Faarup P, Hansen BB, Kristensen AR, Christensen S. C