SavedAdd your journal

Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2018 / Cilt: 24 - Sayı: 5

Development and Evaluation of an ELISA System for Detection of PEDV-Specific IgA Antibodies in Colostrum

Kolostrumda Domuz Epidemik Diare Virus-Spesifik IgA Antikorlarının Belirlenmesinde ELISA Sisteminin Geliştirilmesi ve Değerlendirilmesi

Ren-Feng LI
Xiang-Qin TIAN
Zi-Liang WANG
Jin-You MA
Li-Rong WANG
Shou-Ping ZHANG
Yan-Yan YANG
Jin-Qing JIANG
San-Hu WANG
Yan YU
Pages
717–723
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This test will aid in future diagnostics and assessment of the protective levels of mucosal immune response against PEDV bymeasuring IgA levels in the colostrum.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Porcine epidemic diarrhea virus (PEDV) is a devastating swine infectious disease.
📊
Key Findings & Data:Development of high-performance methods to diagnoseand evaluate viral immune status remains very important to control PEDV.
💡
Conclusion & Impact:This test will aid in future diagnostics and assessment of the protective levels of mucosal immune response against PEDV bymeasuring IgA levels in the colostrum.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Porcine epidemic diarrhea virus (PEDV) is a devastating swine infectious disease. Development of high-performance methods to diagnoseand evaluate viral immune status remains very important to control PEDV. Here, a recombinant S1 protein-based indirect enzyme-linkedimmunosorbent assay (rpS1-iELISA) was developed to monitor IgA antibody in the colostrum. Optimized cut-off value of the rpS1-iELISA wasdetermined as 0.448. Results yielded a sensitivity of 96.87% and a specificity of 100.0%. Repeatability tests indicated that the coefficients ofvariation of the colostrum samples within and between runs were both less than 8%. Test results of 523 field colostrum samples showed thatthe rpS1-iELISA had excellent agreement with immunofluorescence assay (kappa = 0.958) and better test performance than a commercialELISA kit. This test will aid in future diagnostics and assessment of the protective levels of mucosal immune response against PEDV bymeasuring IgA levels in the colostrum.

Özet

Domuz Epidemik Diare Virus (DEDV) enfeksiyonu domuzların oldukça yıkıcı enfeksiyöz bir hastalığıdır. Virusa karşı bağışıklık durumunu değerlendirmek ve tanıyı koyabilmek için yüksek performanslı metotların gelişimi hastalığın kontrol altına alınabilmesi için elzemdir. Bu çalışmada, kolostrumdaki IgA antikorlarını taramak amacıyla rekombinant S1 protein temelli indirekt enzime bağlı immunosorbent metot (rpS1-iELISA) geliştirildi. rpS1-iELISA için optimize edilmiş eşik değeri 0.448 olarak belirlendi. Sonuçlar %96.87 duyarlılık ve %100 özgüllük gösterdi. Tekrarlanabilirlik testleri kolostrum örnekleri ve koşumlar arasında varyasyon katsayısıının %8’den daha az olduğunu gösterdi. Toplam 523 adet saha kolostrum örneklerinin test sonuçları rpS1-iELISA’nın immunofloresans testi (kappa = 0.958) ile mükemmel uyumlulukta olduğunu ve ticari ELISA kitinden daha iyi performansa sahip olduğunu gösterdi. Mevcut test kolostrumda IgA seviyesini ölçerek DEDV’e karşı mukozal bağışıklık yanıtın koruyuculuk seviyesini tahmin etmede ve tanıyı koymada yardımcı olacaktır.

🔖

Cite This Article

LI, R., TIAN, X., WANG, Z., MA, J., WANG, L., ZHANG, S., YANG, Y., JIANG, J., WANG, S., YU, Y. (2022). Development and Evaluation of an ELISA System for Detection of PEDV-Specific IgA Antibodies in Colostrum. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 24 - Sayı: 5, 717–723. https://ajindex.com/articles/569907

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (3)

  1. Gerber PF, Gong Q, Huang YW, Wang C, Holtkamp D, Opriessnig T: Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA. Vet J, 202 (1): 33-36, 2014. DOI: 10.1016/j. tvjl.2014.07.018 Li Y, Zheng F, Fan B, Muhammad HM, Zou Y, Jiang P: Development of an indirect ELISA based on a truncated S protein of the porcine epidemic diarrhea virus. Can J Microbiol, 61 (11): 811-817, 2015. DOI: 10.1139/cjm-2015-0213
  2. Gerber PF, Opriessnig T: Detection of immunoglobulin (Ig) A antibodies against porcine epidemic diarrhea virus (PEDV) in fecal and serum samples. MethodsX, 2, 368-373, 2015. DOI: 10.1016/j.mex. 2015.10.001 Yuan X, Lin H, Li B, He K, Fan H: Efficacy and immunogenicity of recombinant swinepox virus expressing the truncated S protein of a novel isolate of porcine epidemic diarrhea virus. Arch Virol, 162 (12): 3779- 3789, 2017. DOI: 10.1007/s00705-017-3548-1
  3. Song L, Zhang H, Hou P, Wang H, Zhao G, Xia X, He H: Development and preliminary application of an indirect ELISA to detect infectious bovine rhinotracheitis virus using recombinant glycoprotein D of IBRV strain SD. Kafkas Univ Vet Fak Derg, 22 (4): 503-509, 2016. DOI: 10.9775/ kvfd.2015.14890 Kalkanov I, Dinev I, Aleksandrov M, Dimitrov K, Zarkov I: Morphological and etiological investigations in a rotaviral enteritis outbreak in calves. Kafkas Univ Vet Fak Derg, 22 (2): 309-313, 2016. DOI: 10.9775/kvfd.2015.14365 Genç O, Büyüktanir Ö, Yurdusev N: Development of Qualitative and quantitative ELISA models for bovine brucellosis diagnosis. Kafkas Univ Vet Fak Derg, 16 (2): 287-291, 2010. DOI: 10.9775/kvfd.2009.692 Fu Y, Li P, Cao Y, Wang N, Sun P, Shi Q, Ji X, Bao H, Li D, Chen Y, Bai X, Ma X, Zhang J, Lu Z, Liu Z: Development of a blocking elisa using a monoclonal antibody to a dominant epitope in non-structural protein 3A of foot-and-mouth disease virus, as a matching test for a negativemarker vaccine. PLos One, 12 (1): e0170560, 2017. DOI: 10.1371/journal. pone.0170560 Liu C, Kokuho T, Kubota T, Watanabe S, Inumaru S, Yokomizo Y, Onodera T: A serodiagnostic ELISA using recombinant antigen of swine transmissible gastroenteritis virus nucleoprotein. J Vet Med Sci, 63 (11): 1253-1256, 2001. DOI: 10.1292/jvms.63.1253 Jittimanee S, Nuntawan Na Ayudhya S, Kedkovid R, Teankum K, Suradhat S, Thanawongnuwech R: An indirect enzyme-linked immunosorbent assay using a recombinant truncated capsid protein of Porcine circovirus-2. J Vet Diagn Invest, 24 (6): 1129-1132, 2012.