AI Summary & Key Takeaways
AI InsightGSH levels and GPx, SOD activities in both serum and ovarian tissuewere significantly lower in TD group compared to the control and TD-CH groups (P
📌 3 Key Takeaways
Abstract
The objective of this study is to assess the protective eff ect of chrysin (CH) against ovarian torsion-detorsion injury. Thirty-two female albino ratswere divided into 4 groups as Control, Torsion/Detorsion (T/D), TD-CH, and CH. Ovarian torsion was done for 3 hours on TD and TD-CH groupsand then detorsion was performed. 50 mg/kg of CH was administered to the TD-CH group via oral gavage half an hour before the detorsion.Subsequently, 50 mg/kg of CH was administered via oral gavage to TD-CH and CH groups for 14 days. At the end of the experiment, bloodsamples were collected and ovarian tissue were taken. SOD and GPx activities and GSH and 8-OHdG levels were studied in serum and ovariantissue. Also, IL-18, KIM-1, NGAL, Cys-C levels were studied in serum samples. GSH levels and GPx, SOD activities in both serum and ovarian tissuewere significantly lower in TD group compared to the control and TD-CH groups (P
Özet
Bu çalışmadaki amacımız ovaryum torsiyon detorsiyon hasarına karşı chrysinin (CH) koruyucu etkisini değerlendirmektir. Otuz iki adet dişi wistar albino rat 4 gruba ayrıldı. Grup 1. Kontrol grubu, grup 2: TD grubu; grup 3: TD-CH grubu; grup 4: CH grubu. TD ve TD- CH gruplarına 3 saat torsiyon uygulandı ve 3 saatin sonunda detorsiyon yapıldı. TD-CH grubuna detorsiyondan yarım saat önce 50 mg/kg CH oral gavaj ile verildi. Daha sonra TD-CH ve CH gruplarına 14 gün boyunca 50 mg/kg CH oral gavaj ile verildi. Deneme sonunda ratlar sakrifiye edilerek kan örnekleri ve ovaryum dokusu alındı. Serum ve ovaryum dokusunda SOD ve GPx aktiviteleri, GSH ve 8-OHdG seviyeleri, serum örneklerinde ise 8-OHdG, IL-18, KIM-1, NGAL, Cys-C seviyeleri çalışıldı. TD grubunda hem serum hem de ovaryum dokusunda, GSH seviyeleri, GPx ve SOD aktiviteleri kontrol ve TD-CH gruplarından anlamlı olarak düşükken (P