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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2020 / Cilt: 26 - Sayı: 3

A Mutant of Listeria monocytogenes Shows Decreased Virulence and Confers Protection Against Listeriosis in Mice

Farelerde Düşük Virulans Gösteren ve Listeriosise Karşı Koruma Sağlayan Listeria monocytogenes Mutantı

Jingjing REN
Mingwei YANG
Pengyan WANG
Jianjun JIANG
Genqiang YAN
Pages
419–426
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the virulence of Lm90-ΔinlB couldsignificantly decrease compared with the parental strain (Lm90).

📌 3 Key Takeaways

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Objective & Hypothesis:This research was performed to obtain a safe and highly immunogenic Listeria strain and evaluate the biological characteristics of the deletionmutant.
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Key Findings & Data:Based on homologous recombination technology, we constructed a deletion mutant Lm90-ΔinlB of L. monocytogenes.
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Conclusion & Impact:The results showed that the virulence of Lm90-ΔinlB couldsignificantly decrease compared with the parental strain (Lm90).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This research was performed to obtain a safe and highly immunogenic Listeria strain and evaluate the biological characteristics of the deletionmutant. Based on homologous recombination technology, we constructed a deletion mutant Lm90-ΔinlB of L. monocytogenes. Meanwhile, wecharacterized its safety and protective efficacy against listeriosis infection in mice. The results showed that the virulence of Lm90-ΔinlB couldsignificantly decrease compared with the parental strain (Lm90). The deletion strain retained hemolytic activity and induced CD8+ T cell responsecomparable to that of Lm90. Mice immunized with Lm90-ΔinlB were capable of stimulating specific CD8+ T cells to the listerial epitopes LLO91-99and P60217-225 at levels equivalent to Lm90. Importantly, immunization of mice with Lm90-ΔinlB displayed good protection against listeriosis. Inconclusion, strain Lm90-ΔinlB is a vaccine candidate with the potential to be more immunogenic yet considerably less toxic than the parental strain.

Özet

Bu araştırma güvenli ve yüksek derecede immunojenik bir Listeria suşu elde etmek ve delesyon mutantının biyolojik özelliklerini değerlendirmek için yapıldı. Homolog rekombinasyon teknolojisi kullanılarak, L. monocytogenes’in bir delesyon mutantı olan Lm90-ΔinlB oluşturuldu. Aynı zamanda, farelerde listeriosis enfeksiyonuna karşı güvenliği ve koruyucu etkinliği belirlendi. Sonuçlar, Lm90-ΔinlB’in virülansının, parental suşa (Lm90) kıyasla önemli ölçüde azalabileceğini gösterdi. Delesyon mutantı suş hemolitik aktiviteyi korudu ve CD8+ T hücre tepkisini Lm90 ile karşılaştırılabilir düzeyde uyardı. Lm90- ΔinlB ile immünize aşılanmış farelerde, Lm90’a eşdeğer seviyelerde LLO91-99 ve P60217-225 listeria epitoplarına spesifik CD8+ T hücrelerini uyarabilme kapasitesi mevcuttu. Farelerde Lm90-ΔinlB ile aşılanması sonrası listeriyoza karşı iyi bir koruma görülmesi önemli bir bulgudur. Sonuç olarak, Lm90-ΔinlB suşu, parental suşundan daha fazla immünojenik ancak önemli ölçüde daha az toksik olması nedeniyle potansiyel bir aşı adayıdır.

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Cite This Article

REN, J., YANG, M., WANG, P., JIANG, J., YAN, G. (2022). A Mutant of Listeria monocytogenes Shows Decreased Virulence and Confers Protection Against Listeriosis in Mice. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 26 - Sayı: 3, 419–426. https://ajindex.com/articles/569674

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References (1)

  1. Jacks A, Pihlajasaari A, Vahe M, Myntti A, Kaukoranta SS, Elomaa N, Salmenlinna S, Rantala L, Lahti K, Huusko S, Kuusi M, Siitonen A, Rimhanen-Finne R: Outbreak of hospital-acquired gastroenteritis and invasive infection caused by Listeria monocytogenes, Finland, 2012. Epidemiol Infect, 144 (13): 2732-2742, 2016. DOI: 10.1017/s0950268815002563 2. Radoshevich L, Cossart P: Listeria monocytogenes: Towards a complete picture of its physiology and pathogenesis. Nat Rev Microbiol, 16 (1): 32-46, 2018. DOI: 10.1038/nrmicro.2017.126 3. Pagliano P, Ascione T, Boccia G, De Caro F, Esposito S: Listeria monocytogenes meningitis in the elderly: Epidemiological, clinical and therapeutic findings. Infez Med, 24 (2): 105-111, 2016. 4. Pucci L, Massacesi M, Liuzzi G: Clinical management of women with listeriosis risk during pregnancy: A review of national guidelines. Expert Rev Anti-Infect Ther, 16 (1): 13-21, 2018. DOI: 10.1080/14787210.2018.1417837 5. Charlier C, Perrodeau É, Leclercq A, Cazenave B, Pilmis B, Henry B, Lopes A, Maury MM, Moura A, Goffinet F, Dieye HB, Thouvenot P, Ungeheuer MN, Tourdjman M, Goulet V, de Valk H, Lortholary O, Ravaud P, Lecuit M; MONALISA study group: Clinical features and prognostic factors of listeriosis: The MONALISA national prospective cohort study. Lancet Infect Dis, 17, 510-519, 2017. DOI: 10.1016/S1473- 3099(16)30521-7 6. Harty JT, Lenz LL, Bevan MJ: Primary and secondary immune responses to Listeria monocytogenes. Curr Opin Immunol, 8 (4): 526-530, 1996. DOI: 10.1016/S0952-7915(96)80041-0 7. Pamer EG: Immune responses to Listeria monocytogenes. Nat Rev Immunol, 4, 812–823, 2004. DOI: 10.1038/nri1461 8. Jia Q, Bowen R, Dillon BJ, Maslesagalic S, Chang BT, Kaidi AC, Horwitz MA: Single vector platform vaccine protects against lethal respiratory challenge with tier 1 select agents of anthrax, plague, and tularemia. Sci Rep, 8:7009, 2018. DOI: 10.1038/s41598-018-24581-y 9. Yin Y, Tian D, Jiao H, Zhang C, Pan Z, Zhang X, Wang X, Jiao X: Pathogenicity and immunogenicity of a mutant strain of Listeria monocytogenes in the chicken infection model. Clin Vaccine Immunol, 18 (3): 500-505, 2011. DOI: 10.1128/CVI.00445-10 10. Cruz R, Pereira-Castro I, Almeida MT, Moreira A, Cabanes D, Sousa S: Epithelial keratins modulate cMet expression and signaling and promote InlB-Mediated Listeria monocytogenes infection of Hela cells. Front Cell Infect Microbiol, 8:146, 2018. DOI: 10.3389/fcimb.2018.00146 11. Van Ngo H, Bhalla M, Chen DY, Ireton K: A r