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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2022 / Cilt: 28 - Sayı: 2

Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm

Gökkuşağı Alabalığı (Oncorhynchus mykiss) Seminal Plazması İlave Edilmiş Liyofilize Sulandırıcı, Koç Spermasının Dondurulmasını İyileştirir

Zekariya NUR
N. Tekin ÖNDER
Burcu ÜSTÜNER
Eser AKAL
Ahmet AKTAR
Oğuzhan HURAYDIN
Selim ALCAY
M. Melih YILMAZ
Elif GÖKÇE
Muhammed DUMAN
Pages
255–260
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Th e highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P

📌 3 Key Takeaways

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Objective & Hypothesis:Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen.
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Key Findings & Data:Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method.
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Conclusion & Impact:Th e highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. Th e highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P

Özet

Bu çalışma Gökkuşağı alabalığı seminal plazması (ASP) ilave edilmiş (kontrol, %10 ve %15) liyofilize sulandırıcının koç spermasının dondurulabilirliği üzerindeki etkisini belirlemeyi amaçlamıştır. Alınan sperma birleştirilerek, iki eşit hacime bölündü ve ejakülatların her biri, iki aşamalı sulandırma yöntemi kullanılarak ASP (%0, FC ve LC; %10 F10 ve L10 veya %15 F15 ve L15) ilave edilen taze ve liyofilize sulandırıcılarla sulandırıldı. Sperma programlanabilir dondurma makinesi kullanılarak donduruldu. Sperma numuneleri, taze ve eritme sonrası aşamada motilite, akrozom hasarı, plazma membran bütünlüğü ve DNA fragmentasyonu bakımından incelendi. Eritme sonrası en yüksek motilite ve plazma membrane bütünlüğü F10, F15 ve L15 gruplarında gözlendi (P

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Cite This Article

NUR, Z., ÖNDER, N. T., ÜSTÜNER, B., AKAL, E., AKTAR, A., HURAYDIN, O., ALCAY, S., YILMAZ, M. M., GÖKÇE, E., DUMAN, M. (2022). Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 28 - Sayı: 2, 255–260. https://ajindex.com/articles/569454

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References (1)

  1. Ustuner B, Gunay U, Nur Z, Ustuner H: Effects of long and short-term progestagen treatments combined with PMSG on oestrus synchronization and fertility in Awassi ewes during the breeding season. Acta Vet Brno, 76, 391-397, 2007. DOI: 10.2754/avb200776030391 2. Masoudi R, Zare Shahneh A, Towhidi A, Kohram H, Akbarisharif A, Sharafi M: Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen. Cryobiology, 74, 77-80, 2017. DOI: 10.1016/j.cryobiol.2016.11.012 3. Nur Z, Zik B, Ustuner B, Sagirkaya H, Ozguden CG: Effects of different cryoprotective agents on ram sperm morphology and DNA integrity. Theriogenology, 73 (9): 1267-1275, 2010. DOI: 10.1016/j.theriogenology. 2009.12.007 4. Ustuner B, Alçay S, Nur Z, Sağırkaya H, Soylu MK: Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Univ Vet Fak Derg, 20 (3): 393-398, 2014. DOI: 10.9775/kvfd.2013.10248 5. Alcay S, Toker MB, Gokce E, Onder NT, Ustuner B, Nur Z: Long-term incubation resilience of post-thaw ram semen diluted with lecithin-based extender supplemented with bovine serum albumin. Kafkas Univ Vet Fak Derg, 25 (3): 291-297, 2019. DOI: 10.9775/kvfd.2018.20843 6. Pool KR, Rickard JP, De Graaf SP: Melatonin improves the motility and DNA integrity of frozen-thawed ram spermatozoa likely via suppression of mitochondrial superoxide production. Domest Anim Endocrinol, 74:106516, 2021. DOI: 10.1016/j.domaniend.2020.106516 7. Len JS, Koh WSD, Tan SX: The roles of reactive oxygen species and antioxidants in cryopreservation. Biosci Rep, 39 (8): BSR20191601, 2019. DOI: 10.1042/BSR20191601 8. Riesco MF, Alvarez M, Anel-Lopez L, Neila-Montero M, Palacin- Martinez C, Montes-Garrido R, Boixo JC, de Paz P, Anel L: Multiparametric study of antioxidant effect on ram sperm cryopreservation-from field trials to research bench. Animals, 11 (2): 283, 2021. DOI: 10.3390/ani11020283 9. Wu C, Dai J, Zhang S, Sun L, Liu Y, Zhang D: Effect of thawing rates and antioxidants on semen cryopreservation in Hu sheep. Biopreserv Biobank, 19 (3): 204-209, 2021. DOI: 10.1089/bio.2020.0067 10. Jha PK, Shahi Alam MG, Mansur AA, Naher N, Islam T, Uddin Bhuiyan M, Bari FY: Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques. Cryobiology, 89, 35-41, 2019. DOI: 10.1016/j.cryobiol.2019.06.001 11. Sarlos P, Molnar A, Kokai A, Gabor G, Ratky J: Evaluation of the effect of antioxidants in