Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2022 / Cilt: 28 - Sayı: 3

Th e Eff ects of Fast and Slow Th awing on Spermatological Parameters and Detect of Chromatin Condensation by Toluidine Blue Staining in Frozen-Th awed Bull Sperm

Dondurulmuş-Çözdürülmüş Boğa Spermasında Hızlı ve Yavaş Çözdürmenin Spermatolojik Parametreler ve Toluidin Mavisi Boyama ile Kromatin Kondenzasyonunun Belirlenmesi Üzerine Etkisi

Pages
307–313
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Th ere was a significant diff erence (P

📌 3 Key Takeaways

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Objective & Hypothesis:Th e purpose of this study was to observe the eff ect of diff erent thawing methods on semen parameters such as motility and morphology and sperm chromatin integrity as assessed by toluidine blue (TB) staining.
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Key Findings & Data:A total of 20 frozen sperm straws from the same Holstein bull were used.
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Conclusion & Impact:Th ere was a significant diff erence (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Th e purpose of this study was to observe the eff ect of diff erent thawing methods on semen parameters such as motility and morphology and sperm chromatin integrity as assessed by toluidine blue (TB) staining. A total of 20 frozen sperm straws from the same Holstein bull were used. While the 30 sec thawing protocol at 37°C, which is used for thawing frozen sperm straws, constitutes our slow thawing group (n=10), the 6 sec thawing protocol at 70°C constitutes our fast-thawing group (n=10). Th e motility, viability, morphology, plasma membrane integrity, and sperm chromatin condensation parameters of all thawed sperm were investigated. Th ere was a significant diff erence (P

Özet

Bu çalışmanın amacı, farklı çözdürme yöntemlerinin motilite ve morfoloji gibi sperma parametreleri ve toluidin mavisi boyası kullanılarak sperm kromatin bütünlüğü üzerindeki etkisini gözlemlemekti. Aynı Holstein boğasından toplam 20 adet donmuş sperma payeti kullanıldı. Dondurulmuş sperma payetlerinin çözdürülmesinde kullanılan 37°C’de 30 saniyelik çözdürme protokolü yavaş çözdürme grubumuzu (n=10) oluştururken, 70°C’de 6 saniyelik çözdürme protokolü hızlı çözdürme grubumuzu (n=10) oluşturmaktadır. Tüm çözdürülmüş spermaların motilitesi, canlılığı, morfolojisi, plazma membran bütünlüğü ve sperm kromatin yoğunlaşma parametrelerinin analizleri yapıldı. Karşılaştırılan iki grup arasında spermatozoon plazma membran bütünlüğü, baş ve toplam anormal spermatozoon morfolojisinde önemli bir fark gözlendi (P

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Cite This Article

KAYA, C., TAĞRIKULU, M. D., ESİN, B., ÇEVİK, M. (2022). Th e Eff ects of Fast and Slow Th awing on Spermatological Parameters and Detect of Chromatin Condensation by Toluidine Blue Staining in Frozen-Th awed Bull Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 28 - Sayı: 3, 307–313. https://ajindex.com/articles/569451

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References (1)

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