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Journals / Journal of the Turkish-German Gynecological Association / 2017 / Cilt: 18 - Sayı: 3

Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes

Satish Kumar ADİGA
Ramya NAİR
Jyothsna MANİKKATH
Aswathi R. HEGDE
Guruprasad KALTHUR
Pages
102–109
Publication Date
2022-10-18
DOI
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Results: Both DAG and IP3, individually, induced activation in ~98% of oocytes, which was significantly higher (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To explore the ability of diacyl glycerol (DAG) and inositol triphosphate (IP3), two major secondary messengers in the calcium signaling pathway, in activating oocytes.
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Key Findings & Data:Material and Methods: Oocyte cumulus complex obtained from superovulated Swiss albino mice were incubated in M16 medium with liposome-encapsulated 1,2-Dipalmitoyl-sn-glycerol (LEDAG) and/or IP3 for 3 h.
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Conclusion & Impact:Results: Both DAG and IP3, individually, induced activation in ~98% of oocytes, which was significantly higher (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: To explore the ability of diacyl glycerol (DAG) and inositol triphosphate (IP3), two major secondary messengers in the calcium signaling pathway, in activating oocytes. Material and Methods: Oocyte cumulus complex obtained from superovulated Swiss albino mice were incubated in M16 medium with liposome-encapsulated 1,2-Dipalmitoyl-sn-glycerol (LEDAG) and/or IP3 for 3 h. Strontium chloride was used as positive control. The activation potential, ploidy status, and blastocyst rate was calculated. Results: Both DAG and IP3, individually, induced activation in ~98% of oocytes, which was significantly higher (p

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Cite This Article

ADİGA, S. K., NAİR, R., MANİKKATH, J., HEGDE, A. R., KALTHUR, G. (2022). Liposome-encapsulated diacyl glycerol and inositol triphosphate-induced delayed oocyte activation and poor development of parthenotes. Journal of the Turkish-German Gynecological Association, Cilt: 18 - Sayı: 3, 102–109. https://ajindex.com/articles/567516

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