Journals / Journal of the Turkish-German Gynecological Association / 2021 / Cilt: 22 - Sayı: 2

Comparison of the rates for reaching the blastocyst stage between normal and abnormal pronucleus embryos monitored by a time-lapse system in IVF patients

Pages
120–126
Publication Date
2022-10-18
DOI
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⚡ TL;DR(Too Long; Didn't Read)

The proportion reaching blastocyst development was significantly higher following 2PN compared to those after MPN and multiPN (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To compare the rates of blastocyst stage development between embryos fertilized after one (MPN) or more than two pronucleus (PN) (3PN, 4PN-multiPN) with those after 2PN in the same patients.
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Key Findings & Data:Material and Methods: The embryos of patients who had both abnormal PN (MPN, 3PN or 4PN) and normal fertilized (2PN) embryos after intracytoplasmic sperm injection (ICSI) fertilization, were followed with a time-lapse system following the ICSI procedure.
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Conclusion & Impact:The proportion reaching blastocyst development was significantly higher following 2PN compared to those after MPN and multiPN (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: To compare the rates of blastocyst stage development between embryos fertilized after one (MPN) or more than two pronucleus (PN) (3PN, 4PN-multiPN) with those after 2PN in the same patients. Material and Methods: The embryos of patients who had both abnormal PN (MPN, 3PN or 4PN) and normal fertilized (2PN) embryos after intracytoplasmic sperm injection (ICSI) fertilization, were followed with a time-lapse system following the ICSI procedure. The rates of reaching the blastocyst stage were compared between normal and abnormally fertilized embryos. Results: One thousand eight hundred and twenty oocytes were collected from 140 patients and 1280 (70.3%) of them were fertilized. MPN, 2PN and 3PN, 4PN (multiPN) ratios of the embryos in the pronuclear stage were 11.4%, 83.13% and 5.47%, respectively. The rates of reaching the blastocyst stage among these embryos were 17.1%, 60.8% and 42.8% for MPN, 2PN and multiPN, respectively. The proportion reaching blastocyst development was significantly higher following 2PN compared to those after MPN and multiPN (p

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Cite This Article

UYAR, E., TAKMAZ, Ö., SELAM, B., UZUN, K. N., CINCIK, M. (2022). Comparison of the rates for reaching the blastocyst stage between normal and abnormal pronucleus embryos monitored by a time-lapse system in IVF patients. Journal of the Turkish-German Gynecological Association, Cilt: 22 - Sayı: 2, 120–126. https://ajindex.com/articles/567374

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References (17)

  1. Azevedo AR, Pinho MJ, Silva J, Sá R, Thorsteinsdóttir S, Barros A, et al. Molecular cytogenetics of human single pronucleated zygotes. Reprod Sci 2014; 21: 1472-82
  2. Plachot M. Fertilization. Hum Reprod 2000; 15(Suppl 4): 19-30
  3. Kaufman MH. Early Mammalian Development: Parthenogenetic Studies. UK: Cambridge University Press; 1983
  4. Balakier H, Squire J, Casper RF. Characterization of abnormal one pronuclear human oocytes by morphology, cytogenetics and in-situ hybridization. Hum Reprod 1993; 8: 402-8
  5. Staessen C, Janssenwillen C, Devroey P, Steirteghem AC. Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization. Hum Reprod 1993; 8: 221-3
  6. Noyes N, Fino ME, Krey L, McCaffrey C, Adler A, Grifo J. Embryo biopsy: the fate of abnormal pronuclear embryos. Reprod BioMed Online 2008; 17: 782-8
  7. Prados FJ, Debrock S, Lemmen JG, Angerholm I. The cleavage stage embryo. In: ESHRE: Atlas of Human Embryology
  8. Flaherty SP, Payne D, Swann NJ, Matthews CD. Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod 1995; 10: 2623-9
  9. Flaherty SP, Payne D, Matthews CD. Fertilization failures and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod 1998; 13(Suppl 1): 155-64
  10. Rosenbusch B. The chromosomal constitution of embryos arising from monopronuclear oocytes in programmes of assisted reproduction. Reprod Med 2014; 2014: 418198
  11. Papale L, Fiorentino A, Montag M, Tomasi G. The zygote. Hum Reprod 2012; 27 (Suppl 1): i22-49
  12. Feenan K, Herbert M. Can “abnormally” fertilized zygotes give rise to viable embryos?. Hum Fertil (Camb) 2006; 9: 157-69
  13. Rosenbusch BE. A preliminary concept, deduced from cytogenetic analyses, for explaining different types of multipronuclear oocytes obtained after intracytoplasmic sperm injection. Fertil Steril 2010; 94: 2479-81
  14. Moore KL, Persaud TVN, Torchia MG. The Developing Human: Clinically Oriented Embryology. 9th. Philadelphia: Elsevier Saunders; 2013
  15. Chen Z, Yan J, Feng HL. Aneuploid analysis of tripronuclear zygotes derived from in vitro fertilization and intracytoplasmic sperm injection in humans. Fertil Steril 2005; 83: 1845-8
  16. Uyar E, Usal D, Selam B, Cincik M, Bagis T. IVF outcomes after hysteroscopic metroplasty in patients with T- shaped uterus. Fertil Res Pract 2019; 5: 15
  17. Rosenbusch B, Schneider M, Sterzik K. The chromosomal constitution of multipronuclear zygotes r