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Journals / Cukurova Medical Journal / 2018 / Cilt: 43 - Sayı: 3

Evaluation of the cytotoxic and membrane damaging effects of mountain tea (Sideritis stricta Boiss & Heldr.) essential oil on parental and epirubicin-HCl resistant H1299 cells

Parental ve epirubicin-HCl dirençli H1299 hücrelerinde dağ çayı (Sideritis stricta Boiss & Heldr.) uçucu yağının sitotoksik ve membran hasar verici etkilerinin değerlendirilmesi

Ayşe ERDOĞAN
Aysun ÖZKAN
Orhan ÜNAL
Canan DÜLGEROĞLU
Pages
669–677
Publication Date
2018-09-30
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The highest concentration of the essential oil (IC70) treatment caused the highest membrane damage on both parental and drug resistant H1299 cells.Conclusions: Parental and epirubicin-HCl resistant H1299 cells showed different cellular response against potential antitumour and pro-oxidative effects of essential oil.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: In this study, we evaluated Sideritis stricta (S. stricta), as potential-oxidative agents against parental and epirubicin-HCl resistant H1299 cells.
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Key Findings & Data:Assessment of cell viability was made by CellTiter-Blue® Cell Viability Assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay after 20-600 μg/mL essential oil concentrations treated to parental and epirubicin-HCl (drug) resistant H1299 cells for 24, 48 and 72 h.
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Conclusion & Impact:The highest concentration of the essential oil (IC70) treatment caused the highest membrane damage on both parental and drug resistant H1299 cells.Conclusions: Parental and epirubicin-HCl resistant H1299 cells showed different cellular response against potential antitumour and pro-oxidative effects of essential oil.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sideritis stricta · uçucu yağ · membran hasarı

Abstract

Purpose: In this study, we evaluated Sideritis stricta (S. stricta), as potential-oxidative agents against parental and epirubicin-HCl resistant H1299 cells. Material and Methods: Oxidative stress biomarkers such as malondialdehyde level determined in cell lysates. Assessment of cell viability was made by CellTiter-Blue® Cell Viability Assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay after 20-600 μg/mL essential oil concentrations treated to parental and epirubicin-HCl (drug) resistant H1299 cells for 24, 48 and 72 h. Malondialdehyde levels were assayed for determining the membrane damaging effects. Results: Parental H1299 cells were found to be more sensitive to cytotoxic effect of the essential oil. Essential oil showed cytotoxic and more selective effects depend on time and concentration. Essential oil caused increasing malondialdehyde level on both parental and drug resistant H1299 cells. The highest concentration of the essential oil (IC70) treatment caused the highest membrane damage on both parental and drug resistant H1299 cells.Conclusions: Parental and epirubicin-HCl resistant H1299 cells showed different cellular response against potential antitumour and pro-oxidative effects of essential oil.

Özet

Amaç:. Bu çalışmada, Sideritis stricta (S. stricta) parental ve epirubicin-HCl dirençli H1299 hücrelerine karşı potansiyel oksidatif ajan olarak değerlendirilmiştir. Gereç ve Yöntem: Hücre lisatlarında malondialdehit seviyesi gibi oksidatif stres biyobelirteçleri saptanmıştır. Hücre sitotoksisitesinin değerlendirilmesi, 20-600 μg/mL konsantrasyonlarında 24, 48 ve 72 saat parental ve epirubicin-HCl (ilaç) dirençli H1299 hücrelerine uçucu yağ uygulamasından sonra CellTiter-Blue® Hücre Canlılık Testi ve 3- (4,5-dimetil-2-tiazolil) -2,5-difenil-2H-tetrazolyumbromid (MTT) testi ile yapılmıştır. Malondialdehid seviyeleri membran hasar etkisini ortaya koymak için belirlenmiştir.Bulgular: Parental H1299 hücreleri uçucu yağın sitotoksik etkisine karşı daha duyarlı bulunmuştur. Uçucu yağ, zaman ve konsantrasyona bağlı olarak sitotoksik ve daha seçici etkiler göstermiştir. Uçucu yağ, hem parental hem de ilaca dirençli H1299 hücrelerinde malondialdehit seviyesinde artışa neden olmuştur. Uçucu yağın (IC70) en yüksek konsantrasyonu, hem parental hem de ilaca dirençli H1299 hücrelerinde en yüksek membran hasarına neden olmuştur.Sonuç: Parental ve epirubicin-HCl dirençli H1299 hücreleri uçucu yağın potansiyel antitümör ve pro-oksidatif etkilerine karşı farklı hücresel tepkiler göstermiştir.

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Cite This Article

ERDOĞAN, A., ÖZKAN, A., ÜNAL, O., DÜLGEROĞLU, C. (2018). Evaluation of the cytotoxic and membrane damaging effects of mountain tea (Sideritis stricta Boiss & Heldr.) essential oil on parental and epirubicin-HCl resistant H1299 cells. Cukurova Medical Journal, Cilt: 43 - Sayı: 3, 669–677. https://ajindex.com/articles/56589

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References (16)

  1. Marete EN., Jacquier JC, O’Riordan D. Feverfew as a source of bioactives for functional foods: Storage stability in model beverages. J Funct Foods. 2011;3:38-43
  2. Atoui AK, Mansouri A, Boskou G, Kefalas P. Tea and herbal infusions: Their antioxidant activity and phenolic profile. Food Chem. 2005;89:27-36
  3. Rodriguez Vaquer MJ, Tomassini Serravalle LR, Manca de Narda MC, Strasser de Saad AM. Antioxidant capacity and antibacterial activity of phenolic compounds from Argentinean herb infusions. Food Control. 2010;21:779-85
  4. Guimara˜es R, Barros L, Carvalho AM, Ferreira CFR. Infusions and decoctions of mixed herbs used in folk medicine: Synergism in antioxidant potential. Phytother Res. 2011;25:1209-14
  5. Raal A, Orav A, Pussa T, Valner C, Malmiste B, Arak E. Content of essential oil, terpenoids and polyphenols in commercial chamomile (Chamomilla recutita L. Rauschert) teas from different countries. Food Chem. 2012;131:632-8
  6. Kırımer N, Baser KHC, Demirci B, Duman H. Essential oils of Sideritis species of Turkey belonging to the section. Empedoclia Chem Nat Compd. 2004;40:19-23
  7. Küpeli E, Şahin FP, Çaliş İ, Yeşilada E, Ezer N. Phenolic compounds of Sideritis ozturkii and their in vivo anti-inflammatory and antinociceptive activities. J Ethnopharmacol. 2007;112:356-60
  8. Davis PH. Flora of Turkey and East Aegean Islands Volume 7. Edinburgh, UK, Edinburgh University Press, 1982
  9. Davis PH, Mill RR, Tan K. Flora of Turkey and East Aegean Islands Volume 10. Edinburgh, UK, Edinburgh University Press, 1988
  10. Güner A, Özhatay N, Ekim T, Başer KHC Flora of Turkey and East Aegean Islands Volume 11. Edinburgh, UK, Edinburgh University Press, 2000
  11. Topçu G, Gören AC. Biological activity of diterpenoids isolated from Anatolian Lamiaceae plants. Rec Nat Prod. 2007;1:1-16
  12. Halfon B, Goren AC, Ertas A, Topcu G. Complete 13C NMR assignments for Ent-Kaurene diterpenoids from Sideritis species. Magn Reson Chem. 2011;49:291-4
  13. Topçu G, Ertaş A, Öztürk M, Dinçel D, Kılıç T, Halfon B. Ent-kaurane diterpenoids isolated from Sideritis congesta. Phytochem Lett. 2011;4:436-9
  14. Fraga BM. Phytochemistry and chemotaxonomy of Sideritis species from the Mediterranean region. Phytochemistry. 2012;76:7-24
  15. Şahin FP, Ezer N, Alis IC. Terpenic and phenolic compounds from Sideritis stricta. Turk J Chem. 2006;30:495-504
  16. Kırmızıbekmez H, Ariburnu E, Masullo M, Festa M, Capasso A, Yesilada E et al. Iridoid, phenylethanoid and flavonoid glycosides from