Journals / Journal of Limnology and Freshwater Fisheries Research / 2018 / Cilt: 4 - Sayı: 1

Investigating the Effect of Different Growth Media on Biomass Production of Pseudopediastrum boryanum (Turpin) E. Hegewald Isolates

Farklı Besi Ortamlarının Pseudopediastrum boryanum (Turpin) E. Hegewald İzolatlarının Biyokütle Üretimine Etkileri Üzerine Araştırma

Pages
6–12
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Optical density was recorded by using UV-Visible spectrophotometer at670 nm, and cell count examination was performed through drop count method.Besides, dry weight and chlorophyll-a concentration of strain were determined.The highest cell density (3.67x106cells/mL), dry weight (0.032 g/mL) andchlorophyll-a (16.39 µgL-1) production were observed in the Allen medium.Growth rates of P. boryanum were found to be 0.6676 d-1in the Allen and0.6021 d-1in the BG-11 medium.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Microalgae Pseudopediastrum boryanum (Turpin) E.
📊
Key Findings & Data:In the present study, we investigated the growth andbiomass production of P. boryanum through use of semi-continuous culturesemploying two growth media (Allen and BG-11).
💡
Conclusion & Impact:Optical density was recorded by using UV-Visible spectrophotometer at670 nm, and cell count examination was performed through drop count method.Besides, dry weight and chlorophyll-a concentration of strain were determined.The highest cell density (3.67x106cells/mL), dry weight (0.032 g/mL) andchlorophyll-a (16.39 µgL-1) production were observed in the Allen medium.Growth rates of P. boryanum were found to be 0.6676 d-1in the Allen and0.6021 d-1in the BG-11 medium.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Microalgae Pseudopediastrum boryanum (Turpin) E. Hegewald was chosen as asubject for the present research due to its potential uses of wastewater treatmentand biodiesel production. In the present study, we investigated the growth andbiomass production of P. boryanum through use of semi-continuous culturesemploying two growth media (Allen and BG-11). In our previous study, P.boryanum was isolated from different freshwater reservoir through the dilutiontechnique. The isolated P. boryanum strain was inoculated with 270 mL ofmedium + 30 mL of suspension culture and the 16:8 light/dark photoperiod wasapplied. Optical density was recorded by using UV-Visible spectrophotometer at670 nm, and cell count examination was performed through drop count method.Besides, dry weight and chlorophyll-a concentration of strain were determined.The highest cell density (3.67x106cells/mL), dry weight (0.032 g/mL) andchlorophyll-a (16.39 µgL-1) production were observed in the Allen medium.Growth rates of P. boryanum were found to be 0.6676 d-1in the Allen and0.6021 d-1in the BG-11 medium.

Özet

Mikroalg, Pseudopediastrum boryanum (Turpin) E. Hegewald, atık su arıtımı ve biyodizel üretim potansiyelinden dolayı araştırma konusu olarak seçilmiştir. Bu araştırmada, iki besi ortamı (Allen ve BG-11) kullanılarak, kesikli kültür sisteminde P. boryanum'un büyüme ve biyokütle üretimi araştırılmıştır. Önceki çalışmalarımızda, P. boryanum dilüsyon tekniği kullanılarak, farklı tatlısu birikintilerinden izole edilmiştir. İzole edilen P. boryanum suşu, 270 mL besi ortamı + 30 mL süspansiyon kültür ile aşılanmış ve 16:8 aydınlık/karanlık fotoperiyodu uygulanmıştır. Optik yoğunluk 670 nm'de UV-Visible spektrofotometre kullanılarak tespit edilmiş ve suşların hücre sayımı damla sayım metodu kullanılarak yapılmıştır. Kültürlerin kuru ağırlık ve klorofil-a tayinleri de gerçekleştirilmiştir. Allen ortamında en yüksek hücre yoğunluğu (3,67x106 hücre/mL), kuru ağırlık (0,032 g/mL) ve klorofil-a (16,39 μg L-1 ) olarak tespit edilmiştir. P. boryanum’un büyüme oranları Allen besi ortamında 0,6676 d-1 ve BG-11 ortamında 0,6021 d-1 olarak bulunmuştur.

🔖

Cite This Article

ERKAYA, İ. A., ÖZER, T., DUYGU, D. Y. (2022). Investigating the Effect of Different Growth Media on Biomass Production of Pseudopediastrum boryanum (Turpin) E. Hegewald Isolates. Journal of Limnology and Freshwater Fisheries Research, Cilt: 4 - Sayı: 1, 6–12. https://ajindex.com/articles/564212

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (1)

  1. Ak I, Cirik S, Goksan T. 2008. Effect of light intensity, salinity and temperature on growth in Camalt strain of Dunaliella viridis Teodoresco from Turkey. J Biol Sci. 8(8): 1356-1359. doi: 10.3923/jbs.2008.1356.1359 Al-Shatri AHA, Ali E, Al-Shorgani NKN, Kalil MS. 2014. Growth of Scenedesmus dimorphus in different algal media and pH profile due to secreted metabolites. Afr J Biotechnol. 13(16):1714-1720. doi:10.5897/AJB2013.13455 Baykal Ozer T. Acıkgoz Erkaya. I. Udo Udoh A. Yalcın Duygu D, Akbulut A, Bayramoglu G, Arica MY. 2012. Biosorption of Cr (VI) by free and immobilized Pediastrum boryanum biomass: equilibrium, kinetic, and thermodynamic studies. Environ Sci Pollut Res. 19(7): 2983-2993. doi: 10.1007/s11356-012-0809-0 Bourrelly P. 1972. Les Algues D’eau Douce, Tome I: Les Algues Vertes E’ditions N. Boubee & Cie 3, France: Place Saint Andre Des Arts 572p. Brennan L, Owende P. 2010. Biofuels form microalgae - a review of technologies for production, processing and extractios of biofuels and co-products. Renew Sust Energ Rev. 14(2): 557-577. doi: 10.1016/j.rser.2009.10.009 Bricaud A, Morel A, Babin M, Allali K, Claustre H. 1998. Variations of light absorption by suspended particles with chlorophyll a concentration in oceanic (case 1) eaters: analysis and implication for biooptical models. J Geophys Res. 103(13): 31033- 31044. doi: 10.1029/98JC02712 Chia MA, Lombardi AT, Melao MGG. 2013. Growth and biochemical composition of Chlorella vulgaris in different growth media. Annals of the Brazilian Academy of Sciences 85(4): 1427-1438. doi: 10.1590/0001-3765201393312 CSIRO 2017. Commonwealth Scientific and Industrial Research Organization. [Cited 2017 July]. Available from http://www.csiro.au/. Duygu Yalçin D. 2017. Increasing the protein amount of Chlorella vulgaris [Chlorphyta] strains isolated from different fresh water ponds. Journal of Environmental Science and Engineering B(6): 201-208. doi:10.17265/2162-5263/2017.04.003 Esakkimuthu S, Krishnamurthy V, Govindarajan R, Swaminathan K. 2016. Augmentation and starvation of calcium, magnesium, phosphate on lipid production of Scenedesmus obliquus. Biomass Bioenerg. 88: 126-134. doi: 10.1016/j.biombioe.2016.03.019 Fakhri M, Arifin NB, Budianto B, Yuniarti A, Hariati AM. 2015. Effect of salinity and photoperiod on growth of microalgae Nannochloropsis sp. and Tetraselmis sp. Nature Environment and Pollution Technology 14(3): 563-566. Falkowski PG. 1984, Physiological responses of phytoplankton to natural ligh