Journals / Journal of FisheriesSciences.com / 2010 / Cilt: 4 - Sayı: 1

The existence of Flavobacterium spp. at rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) hatcheries in Caglarca village, Mersin-Turkey

Mersin ili Çağlarca Köyündeki Gökkuşağı alabalığı (Oncorhynchus mykiss, walbaum, 1792) kuluçkahanelerinde Flavobacterium spp. varlığı

Pages
112–122
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In according to antimicrobial susceptibility test, Flavobacterium spp. strains were sensitive to gentamicin, streptomycin, neomycin, sulphamethoxazoletrimethoprim, oxytetracycline, ofloxacin, clindamycin, amoxycilin/clavulinic acid, and resistant to erythromycine, penicillin and vancomycin, respectively

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to examine the existence of the Flavobacterium spp. at two different rainbow trout farm hatcheries in Caglarca village, Mersin, between December 2006 and April 2007.
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Key Findings & Data:For this goal samples were collected from milt, ovarian fluid, and all phases from egg till 3 months old fries and from the water used in both farms.
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Conclusion & Impact:In according to antimicrobial susceptibility test, Flavobacterium spp. strains were sensitive to gentamicin, streptomycin, neomycin, sulphamethoxazoletrimethoprim, oxytetracycline, ofloxacin, clindamycin, amoxycilin/clavulinic acid, and resistant to erythromycine, penicillin and vancomycin, respectively
🔬Design:Research Article
📍Scope:2006 · 347 samples
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to examine the existence of the Flavobacterium spp. at two different rainbow trout farm hatcheries in Caglarca village, Mersin, between December 2006 and April 2007. For this goal samples were collected from milt, ovarian fluid, and all phases from egg till 3 months old fries and from the water used in both farms. In consequence of disease outbreak at the II. farm, 30–40 days after fry started feeding, mortality occured as high as 50 %. Clinical signs at fries were observed as dark coloration of the skin, exophthalmia, erosions on dorsal and caudal fins, lethargy, swimming close to the water surface, weakness and loss of appetite. As autopsy signs anaemia and haemorrhage on gills and liver, and splenomegali were observed. Totally 347 samples, including 77 water and 270 fish samples taken from the hatcheries, were examined. Flavobacterium spp. were isolated and identified using conventional culture methods. 141 (33.71%) Flavobacterium spp. have been isolated from 117 samples. 46 Flavobacterium spp. were isolated from 39 (50.64%) water samples and 95 from 78 (28.88%) fish samples. Off the total isolates 76 (42.93%) were identified as F. aquatile, 30 (16.94%) as F. columnare, 20 (11.29%) as F. branchiophilum, 12 (6.77%) as F. johnsoniae and 3 (1.69%) as F. saccarophilum. Antimicrobial susceptibility test was carried out by agar disc diffusion method. In according to antimicrobial susceptibility test, Flavobacterium spp. strains were sensitive to gentamicin, streptomycin, neomycin, sulphamethoxazoletrimethoprim, oxytetracycline, ofloxacin, clindamycin, amoxycilin/clavulinic acid, and resistant to erythromycine, penicillin and vancomycin, respectively

Özet

Bu araştırma ile Mersin İli Çağlarca Köyü’ndeki iki farklı Gökkuşağı alabalığı (Oncorhynchus mykiss) işletmesinin kuluçkahanelerinde Flavobacterium spp. varlığının aranması hedeflenmiştir. Bu amaçla, Aralık 2006 ve Nisan 2007 tarihleri arasında sperma, ovaryum sıvısı, yumurta, 3 aylık oluncaya kadar yavruların tüm gelişim basamaklarından ve işletmelerde kullanılan sudan örnekler alınmıştır. Yavrular yem almaya başladıktan 30–40 gün sonra II. işletmede görülen salgın sonucunda mortalite % 50 olarak gerçekleşmiştir. Hastalık bulgusu olarak renkte kararma, eksoftalmus, sırt yüzgecinde ve kuyruk yüzgecinde erozyonlar, uyuşukluk, halsizlik, su yüzeyine yakın yüzme ve yem alma oranında azalma gözlemlenmiştir. Otopsi bulgusu olarak solungaçlarda ve karaciğerde anemi ve hemoraji, dalakta büyüme tespit edilmiştir. Her iki işletmenin kuluçkahanesinden alınan 77 adet su örneği ve 270 adet balık örneği olmak üzere toplam 347 adet örnek çalışılmıştır. Flavobacterium spp. izolasyon ve identifikasyonu klasik yöntemler kullanılarak yapılmıştır. Toplam 117 örnekten (% 33.71) 141 adet Flavobacterium spp. izole edilmiştir. Su örneklerinin 39’unda (% 50.64) 46 adet ve balık örneklerinin 78’inde (% 28.88) 95 adet Flavobacterium spp. belirlenmiştir. Toplam izolatın 76’sı (% 42.93) F. aquatile, 30’u (% 16.94) F. columnare, 20’si (% 11.29) F. branchiophilum, 12’si (% 6.77) F. johnsoniae ve 3’ü (% 1.69) de F. saccarophilum olarak belirlenmiştir. Antimikrobiyel duyarlılık testi disk difüzyon yöntemine göre yapılmıştır. Antimikrobiyel duyarlılık testlerine göre Flavobacterium spp. izolatlarının sırasıyla gentamisin, streptomisin, neomisin, sulfametoksazol-trimetoprim, ofloksasin, oksitetrasiklin, klindamisin, amoksisilin/klavulinik asit’e duyarlı ve eritromisin, penisilin ve vankomisin’e dirençli oldukları görülmüştür.

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Cite This Article

ÖZER, S., YILDIRIM, S. (2022). The existence of Flavobacterium spp. at rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) hatcheries in Caglarca village, Mersin-Turkey. Journal of FisheriesSciences.com, Cilt: 4 - Sayı: 1, 112–122. https://ajindex.com/articles/563281

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