SavedAdd your journal

Journals / Cukurova Medical Journal / 2020 / Cilt: 45 - Sayı: 1

Effects of glutamine and β-hydroxy β-methyl butyrate on methotrexate induced intestinal mucositis

Glutamin ve β-hidroksi β-metil bütiratin metotreksatin indüklediği intestinal mukozit üzerine etkisi

Mehmet ALAKAYA
Elvan Çağlar ÇITAK
Simge TAŞKINLAR
Sakir Necat YILMAZ
Ahmet Ata ÖZÇİMEN
Mehmet Yavuz Burak ÇİMEN
Pages
266–271
Publication Date
2020-03-31
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: This research showed that the combination of Gln and HMB use is more effective then the separate use of both chemicals.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Purpose: We aimed to evaluate preventing effects of Glutamine (Gln) and β-hydroxy β-methylbutyrate (HMB) on methotrexate induced intestinal mucositisMaterials and Methods: Fifty Wistar albino rats were divided into 5 groups (G).
📊
Key Findings & Data:Group G1 defined as control and G2 was the methotrexate (MTX) treated group.
💡
Conclusion & Impact:Conclusion: This research showed that the combination of Gln and HMB use is more effective then the separate use of both chemicals.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Glutamin · β-hidroksi β-metilbutirat · metotreksat

Abstract

Purpose: We aimed to evaluate preventing effects of Glutamine (Gln) and β-hydroxy β-methylbutyrate (HMB) on methotrexate induced intestinal mucositisMaterials and Methods: Fifty Wistar albino rats were divided into 5 groups (G). Group G1 defined as control and G2 was the methotrexate (MTX) treated group. The group G3, G4 and G5 were MTX plus Gln, Mtx plus HMB, MTX plus Gln and HMB respectively. Distilled water was applied by gavage to the first 2 groups. Rat received intraperitoneal injections of MTX at the third day. On the fifth day intestinal tissue samples were obtained. Results: The G5 had similar results with the G1 group in the crypt length. According to Park scoring, G1 and G5 were both the highest scores among five study groups. When the tissue was inspected by caspase-3 coating, the lowest apoptotic rate is found in the G5. Conclusion: This research showed that the combination of Gln and HMB use is more effective then the separate use of both chemicals.

Özet

Amaç: Metotreksatın sebep olduğu intestinal mukozit üzerinde Glutamin (Gln) ve β-hidroksi β-metilbutiratın (HMB) önleyici etkilerini değerlendirmeyi amaçladıkGereç ve Yöntem: 50 Wistar albino cinsi sıçan 5 gruba (G) ayrıldı. G1 kontrol grubu ve G2 metotreksat (MTX) ile tedavi edilen gruptu. G3, G4 ve G5 sırasıyla MTX ve Gln, Mtx ve HMB, MTX ve Gln, HMB verilen gruplardı. Sıçanlara üç gün intraperitoneal MTX uygulandı. Beşinci günde bağırsak doku örnekleri alındı.Bulgular: G5 kript derinlik ölçümleri G1 grubu ile benzerdi. Park skorlamasına göre G1 ve G5, beş çalışma grubu arasında en yüksek skoru alan gruplardı. Doku kaspaz-3 ile incelendiğinde, en düşük apoptotik oran G5'te bulundu.Sonuç: Bu çalışma, Gln ve HMB kullanım kombinasyonunun her iki kimyasal maddenin ayrı kullanımından daha etkili olduğunu göstermiştir

🔖

Cite This Article

ALAKAYA, M., ÇITAK, E. Ç., TAŞKINLAR, S., YILMAZ, S. N., ÖZÇİMEN, A. A., ÇİMEN, M. Y. B. (2020). Effects of glutamine and β-hydroxy β-methyl butyrate on methotrexate induced intestinal mucositis. Cukurova Medical Journal, Cilt: 45 - Sayı: 1, 266–271. https://ajindex.com/articles/56235

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Rubenstein EB, Peterson DE, Schubert M, Keefe D, McGuire D, Epstein J et al. Clinical practice guidelines for the prevention and treatment of cancer therapyinduced oral and gastrointestinal mucositis. Cancer 2004;100:2026–46
  2. Pico JL, Avila-Garavito A, Naccache P. Mucositis: its occurrence, consequences, and treatment in the oncology setting. The Oncologist 1998;3:446–51
  3. Sonis ST, Elting LS, Keefe D, Peterson DE, Schubert M, Hauer-Jensen M et al. Perspectives on cancer therapy-induced mucosal injury: Pathogenesis, measurement, epidemiology, and consequences for patients. Cancer 2004;100:1995–2025 4. van Zanten AR., Dhaliwal R., Garrel D., Heyland DK. Enteral glutamine supplementation in critically ill patients: a systematic review and meta-analysis. Crit Care. 2015;19:294
  4. Neu J, Li N. Pathophysiology of glutamine and glutamate metabolism in premature infants. Curr Opin Clin Nutr Metab Care. 2007;10:75-9
  5. Sido B, Seel C, Hochlehnert A, Breitkreutz R, Dröge W. Low intestinal glutamine level and low glutaminase activity in Crohn's disease: a rational for glutamine supplementation? Dig Dis Sci. 2006;51:2170–9. 7. van der Hulst RR, van Kreel BK, von Meyenfeldt MF, Brummer RJ, Arends JW, Deutz NE et al. Glutamine and the preservation of gut integrity. Lancet. 1993;341:1363–5
  6. Coeffier M, Dechelotte P. The role of glutamine in intensive care unit patients: mechanisms of action and clinical outcome. Nutr Rev. 2005;63:65–9
  7. Pimentel GD, Rosa JC, Lira FS, Zanchi NE, Ropelle ER, Oyama LM et al. β-Hydroxy-β-methylbutyrate (HMB) supplementation stimulates skeletal muscle hypertrophy in rats via the mTOR pathway. Nutr Metab 2011;8:11-18
  8. Nissen S, Sharp R, Ray M, Rathmacher JA, Rice D, Fuller JC Jr, et al. Effect of leucine metabolite betahydroxy-beta-methylbutyrate on muscle metabolism during resistance-exercise training. J Appl Physion 1996;81:2095-2104
  9. Nunes EA, Kuczera D, Brito GA, Bonatto SJ, Yamazaki RK, Tanhoffer RA et al. Beta-hydroxybeta-methylbutyrate supplementation reduces tumor growth and tumor cell proliferation ex vivo and prevents cachexia in Walker 256 tumor-bearing rats by modifying nuclear factor-kappaB expres-sion. Nutr Res. 2008;28:487-93
  10. Williams JZ, Abumrad N, Barbul A. Effect of a specialized amino acid mixture on human collagen deposition. Ann Surg. 2002;236:369-74
  11. Park PO, Haglund U, Bulkley GB, Fält K. The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion.