Novel oxidative stress biomarker in sepsis: dynamic thiol-disulfide homeostasis

Objective & Hypothesis:Purpose: The aim of this study was to investigate the relationship between serum dynamic thiol-disulfide homeostasis (TDH) and sepsis, in adult patients.Materials and Methods: In this observational, prospective, case-control study patients (n=44) diagnosed with sepsis compared with ages and gender-matched healthy controls (n=44).

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Key Findings & Data:Patients were divided into two subgroups (survivors and non-survivors) dependent variable being remaining alive on the 28th day after ICU admission at the follow-up period.

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Conclusion & Impact:Conclusion: This study showed that impairment in dynamic TDH in adults with sepsis may be related to negative outcomes.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Dinamik Tiyol-Disülfir Homeostazisi · Sepsis · Oksidatif stres.

Abstract

Purpose: The aim of this study was to investigate the relationship between serum dynamic thiol-disulfide homeostasis (TDH) and sepsis, in adult patients.Materials and Methods: In this observational, prospective, case-control study patients (n=44) diagnosed with sepsis compared with ages and gender-matched healthy controls (n=44). Patients were divided into two subgroups (survivors and non-survivors) dependent variable being remaining alive on the 28th day after ICU admission at the follow-up period. TDH parameters measured using a novel automatic and spectrophotometric method and compared statistically.Results: In patients with sepsis, native thiol, total thiol, and disulfide levels were lower than the control group, and this difference was statistically significant. TDH parameters between surviving and non-surviving patients were similar at the sepsis diagnosis. However, significant differences found in native and total thiol levels at follow-up via repeated measurement analyses on days 3 and 7 in surviving and non-surviving sub-groups. Conclusion: This study showed that impairment in dynamic TDH in adults with sepsis may be related to negative outcomes.

Özet

Amaç: Bu gözlemsel, prospektif, vaka kontrol çalışmasında erişkin hastalarda serum dinamik Tiyol-Disülfir Homeostazisi (TDH) ile sepsis arasındaki ilişkiyi araştırmayı amaçladık.Gereç ve Yöntem: Sepsis tanısı ile alan 44 hasta, yaş ve cinsiyet uyumlu 44 sağlıklı kontrol ile karşılaştırıldı. Hastalar yoğun bakım ünitesi yatışından sonraki 28. günde sağ-kalan ve hayatını kaybedenler olarak 2 gruba ayrıldı. TDH parametreleri yeni, otomatik ve spektrofotometrik bir yöntem kullanılarak ölçüldü ve sonuçlar istatistiksel olarak karşılaştırıldı.Bulgular: Sepsisli hastalarda, nativ tiyol, total tiyol ve disülfit seviyeleri kontrol grubuna göre daha düşük olarak bulundu ve bu fark istatistiksel olarak anlamlı idi. Başlangıç TDH parametreleri sağ-kalan ve hayatını kaybeden hastalar arasındaki benzer bulundu. Ancak sağ kalan ve ex olan hastaların 3 ve 7. günde tekrarlanan TDH ölçümleri (nativ ve toplam tiyol seviyeleri) arasında anlamlı farklar bulundu.Sonuç: Bu çalışma, erişkin sepsis hastalarında dinamik TDH'deki bozulmanın negatif sonuçlara ilişkili olabileceğini göstermiştir.

Keywords

Dinamik Tiyol-Disülfir Homeostazisi Sepsis Oksidatif stres.Dynamic thiol-disulfide homeostasis

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Cite This Article

KOZANHAN, B., EĞİLMEZ, A. İ., CEKMECELİOGLU, B. T., TUTAR, M. S., EREN, G., ÖZEL, D. B., DENİZ, Ç. D., NEŞELİOĞLU, S., EREL, O. (2020). Novel oxidative stress biomarker in sepsis: dynamic thiol-disulfide homeostasis. Cukurova Medical Journal, Cilt: 45 - Sayı: 1, 63–70. https://ajindex.com/articles/56208

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