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Journals / Cukurova Medical Journal / 2020 / Cilt: 45 - Sayı: 2

Plasma expression and methylation levels of vascular endothelial growth factor (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia in Çukurova Region, Turkey

Çukurova Bölgesinde akut lenfoblastik lösemili çocuklarda vasküler endotelyal büyüme faktörü (VEGF-C) ve temel fibroblast büyüme faktörü (bFGF) plazma ekspresyonu ve metilasyon seviyeleri

Nihal İNANDIKLIOGLU
Osman DEMİRHAN
İbrahim BAYRAM
Atila TANYELİ
Pages
581–587
Publication Date
2020-06-30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further studies are required to establish strong links with both VEGF-C and bFGF, and ALL risk factors.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: The aim of this study was to determine the expression and methylation levels of vascular endothelial growth factor-C (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia (ALL).Materials and Methods: We detected mRNA expression profiles and methylation levels of VEGF-C and bFGF genes in the peripheral blood samples of 26 patients with newly diagnosed ALL and 26 healthy controls by real-time quantitative PCR.
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Key Findings & Data:Results: The expression level of bFGF was significantly increased (4.23-fold) in ALL patients as compared with controls.
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Conclusion & Impact:Further studies are required to establish strong links with both VEGF-C and bFGF, and ALL risk factors.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:acute lymphoblastic leukemia · basic fibroblast growth factor · gene expression

Abstract

Purpose: The aim of this study was to determine the expression and methylation levels of vascular endothelial growth factor-C (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia (ALL).Materials and Methods: We detected mRNA expression profiles and methylation levels of VEGF-C and bFGF genes in the peripheral blood samples of 26 patients with newly diagnosed ALL and 26 healthy controls by real-time quantitative PCR. Results: The expression level of bFGF was significantly increased (4.23-fold) in ALL patients as compared with controls. Moreover, VEGF-C were significantly decreased (3.41-fold) in ALL patients as compared with controls. The methylation of the promoter region of VEGF-C (6.88%) and bFGF (16.64%) genes was higher in ALL patients than in healthy controls. Conclusion: Long-term changes in VEGF-C and bFGF at different time intervals as a result of years of follow-up of patients may show stronger associations with disease risk. Further studies are required to establish strong links with both VEGF-C and bFGF, and ALL risk factors.

Özet

Amaç: Bu çalışmanın amacı akut lenfoblastik lösemili (ALL) çocuklarda vasküler endotel büyüme faktörü-C (VEGF-C) ve temel fibroblast büyüme faktörünün (bFGF) ekspresyon ve metilasyon düzeylerini belirlemekti.Gereç ve Yöntem: Gerçek zamanlı kantitatif PCR ile yeni tanı almış olan 26 hasta ve 26 sağlıklı kontrolün periferik kan örneklerinde VEGF-C ve bFGF genlerinin mRNA ekspresyon profilleri ve metilasyon seviyelerini tespit ettik.Bulgular: bFGF ekspresyon seviyesi ALL hastalarında kontrollere kıyasla anlamlı derecede artmıştı (4.23 kat). Ayrıca, VEGF-C ALL hastalarında kontrollere kıyasla anlamlı derecede azalmıştı (3.41 kat). VEGF-C (%6.88) ve bFGF (%16.64) genlerinin promotor bölgelerinin metilasyon oranı ALL hastalarında sağlıklı kontroller göre daha yüksekti.Sonuç: Hastaların yıllar süren takibi sonucunda VEGF-C ve bFGF'de farklı zaman aralıklarında uzun süreli değişiklikler hastalık riski ile daha güçlü ilişkiler gösterebilir. Hem VEGF-C hem de bFGF ve ALL risk faktörleri ile güçlü bağlantılar kurmak için daha ileri çalışmalara ihtiyaç vardır.

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Cite This Article

İNANDIKLIOGLU, N., DEMİRHAN, O., BAYRAM, İ., TANYELİ, A. (2020). Plasma expression and methylation levels of vascular endothelial growth factor (VEGF-C) and basic fibroblast growth factor (bFGF) in children with acute lymphoblastic leukemia in Çukurova Region, Turkey. Cukurova Medical Journal, Cilt: 45 - Sayı: 2, 581–587. https://ajindex.com/articles/56180

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