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Journals / Journal of Cell and Molecular Biology / 2002 / Cilt: 1 - Sayı: 1

Türkiye, Marmara bölgesinden bir populasyon örneğinde üç STR lokusunun (D16S539, D7S820, D13S317) analizi

Analysis of the three STR loci (D16S539, D7S820, D13S317) in a population sample of Marmara region of Turkey

Bahar TAŞDELEN
A. Hadi ÇAKIR
Fazilet ŞİMŞEK
Ayşen ÇELEBİOĞLU
Pages
25–30
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Population data showed a combined discrimination power of 0.9993 and a combined power of exclusion of 0.9523.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, allele and genotype frequencies for the short tandem repeat (STR) loci D16S539, D7S820, and D13S317 were investigated in 138 unrelated individuals (124 for D13S317, 125 for D7S820, 138 for D16S539) living in Marmara region by PCR technique using an allelic ladder consisting of these loci as a standard size marker.
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Key Findings & Data:Amplified producs were separated on 6% denaturing polyacrylamide gel, visualised by silver staining.
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Conclusion & Impact:Population data showed a combined discrimination power of 0.9993 and a combined power of exclusion of 0.9523.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, allele and genotype frequencies for the short tandem repeat (STR) loci D16S539, D7S820, and D13S317 were investigated in 138 unrelated individuals (124 for D13S317, 125 for D7S820, 138 for D16S539) living in Marmara region by PCR technique using an allelic ladder consisting of these loci as a standard size marker. It was evaluated that these STR loci could be valuable for forensic and paternity identification and the results were compared with other population studies. These loci were simultaneously amplified according to Gene Print™ Silver STR™ triplex kit (Promega Corp.). Amplified producs were separated on 6% denaturing polyacrylamide gel, visualised by silver staining. Seven alleles (8-14) were identified for D16S539, eight alleles (7-14) for D7S820, and seven alleles (8-14) for D13S317. No new alleles were found. The population investigated showed no deviation from Hardy-Weinberg equilibrium in the loci D16S539, D7S820 and D13S317. Population data showed a combined discrimination power of 0.9993 and a combined power of exclusion of 0.9523.

Özet

Bu çalışmada, Marmara bölgesinde yaşayan toplam 138 (D13S317 için 124, D7S820 için 125 ve D16S539 için 138 birey) akraba olmayan bireyden oluşan bir örnekte D16S539, D7S820 ve D13S317 STR lokuslarının allel ve genotip frekansları PCR tekniğiyle allelik cetvel kullanılarak belirlenmiştir. Belirtilen STR lokuslannın bu bölgede kriminal amaçli kimliklendirme ve akrabalık ilişkilerinin tespitinde kullanılabilirliği istatistiksel olarak değerlendirilmiş ve elde edilen sonuçlar farklı populasyon çalışmalan ile karşılaştrnlmıştır. Bu lokuslar, Gene PrintTM Silver STR (Promega Corp.) kitine göre PCR, denature poliakrilamid jel elektroforezi ve gümüş boyama yöntemi ile analiz yapılmıştır. Çalışmada D16S539 lokusu için 7 allel (8-14), D7S820 lokusu için 8 allel (7-14) ve D13S317 lokusu için 7 allel (8-14) tanımlanmıştır. Yeni allel bulunmamış olup, populasyonun bu lokuslarda Hardy-Weinberg Eşitliğine göre dengede olduğu görülmüştür. 3 STR lokusun birlikte ayrım gücü 0.9993, dışlama gücü ise 0,9523 olarak hesaplanmıştır.

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Cite This Article

TAŞDELEN, B., ÇAKIR, A. H., ŞİMŞEK, F., ÇELEBİOĞLU, A. (2022). Türkiye, Marmara bölgesinden bir populasyon örneğinde üç STR lokusunun (D16S539, D7S820, D13S317) analizi. Journal of Cell and Molecular Biology, Cilt: 1 - Sayı: 1, 25–30. https://ajindex.com/articles/560726

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