Journals / İstanbul Üniversitesi Veteriner Fakültesi Dergisi / 2004 / Cilt: 30 - Sayı: 1

A study on the pseudomonad septicemia in young rainbow trout (Oncorhynchus mykiss)

Yavru alabalıklarda (Oncorhynchus mykiss) pseudomonad septisemisi üzerine bir çalışma

Pages
121–131
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition to this findings diffuse oedema among the liver cells and focal necrosis in the liver and kidney lubul epithelium cells, necrosis in the gill epithelium and haemorrhagie were also seen.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the aetiological agent and histopathological changes of a infectious diseases induced high mortality in our Faculty Rainbow Trout Hatchery Unite fish (2-3 months old) located in Sapanca were investigated.
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Key Findings & Data:The bacterial colonies were obtained only from kidney inoculation after 5 days period incubation at 20°C.
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Conclusion & Impact:In addition to this findings diffuse oedema among the liver cells and focal necrosis in the liver and kidney lubul epithelium cells, necrosis in the gill epithelium and haemorrhagie were also seen.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, the aetiological agent and histopathological changes of a infectious diseases induced high mortality in our Faculty Rainbow Trout Hatchery Unite fish (2-3 months old) located in Sapanca were investigated. Bacteriological inoculations were made from kidney, liver and spleen to Tryptic Soy Ağar (TSA) from the infected fish visceral organs. The bacterial colonies were obtained only from kidney inoculation after 5 days period incubation at 20°C. The isolate were comprised motile Gram negative rod shaped bacteria those strictly oxidative by the O/F glucose test, cytocrome oxidase positive and produced fluoresce under ultraviolet light and identified as Pseudamonaxjliiorescens. Affected fish showed darkening of the skin, fin or tail rot, haemorrhagic lesions on the skin and the base of the fins, anorexia, exophtalmia. Main histopathological changes were localized in the interstitial haemopoietic tissue of kidney and in the muscle cells of the underlying dermiş. In addition to this findings diffuse oedema among the liver cells and focal necrosis in the liver and kidney lubul epithelium cells, necrosis in the gill epithelium and haemorrhagie were also seen.

Özet

Bu çalışma Fakültemize bağlı Sapanca Gökkuşağı Alabalık Üretim Ünitesi'ndeki 2-3 aylık genç alabalıklarında 2002 yılının Mart ayında görülen ve yüksek mortaliteye neden olan enteksiyöz hastalığın etkenini ve histopatolojik değişiklikleri tespit etmek için yürütülmüştür. Bakteriye! ekimler enfekte balıkların böbrek, karaciğer ve dalak gibi viseral organlarından Tryptic Soy Ağar (TSA) besiyerine yapılmıştır. Hastalık etkeni olan bakteri sadece böbrekten yapılan ve 20°C'de 5 gün süre ile inkübe edilen besiyerinde izole edilebilmiştir, tzole edilen bakteri, hareketli, Gram negatif çomak şekilli basilden oluşup, sitokronı oksidaz pozitif ve O/F gltıkoz testinde kuvvetli oksidatif olduğu ve ultraviyole ışık' altında floresans oluşturduğu için Pseııdoınonas fluorescens olarak idaııtifıye edilmiştir. Histopatolojik olarak başlıca patolojik değişiklikler derinin dermiş tabakası altındaki kas hücrelerinde ve böbreğin interrenal haemopoietik dokusunda gözlenmiştir. Bunlara ek olarak karaciğer hücreleri arasında yaygın ödem, karaciğer ve böbrek epitel hücrelerinde fokal nekroz ve solungaç epitel hücrelerinde nekroz ve hemoraji görülmüştür.

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Cite This Article

AKAYLI, T., TİMUR, G. (2022). A study on the pseudomonad septicemia in young rainbow trout (Oncorhynchus mykiss). İstanbul Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 30 - Sayı: 1, 121–131. https://ajindex.com/articles/555375

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References (2)

  1. Austin, B., Austin, D.A.: Pseudomonads. Bacterial Fish Pathogens: Disease in Farmed and Wild Fish. Chapter 12. Ellis Harword Ltd., 1987; 251-262. 2.Austin, B.: Bacteria of Fish. In: The Pseudomonadaceae. Methods in Aquatic Bacteriology. Ed. B. Austin, Chapter 5, Edinburg, 1988; 123-124. 3.Balebona, M.C., Zorrilla, I., Morinigo, M.A., Borrego, J.J.: Survey of Bacterial pathologies affecting farmed gilt-head sea bream (Spams aurata L.) in southwestern Spain From 1990 to 1996. Aquaculture 1988; 166, 19-35. 4.Bullock, A.M.: Laboratory Methods in Fish Pathology. Ed. by Roberts R.J., Bailliere Tindall, London, 1978; 235-267. 5.Culling, C.F.A.: Handbook of Histopathological Techniques. Second edition, Ed. H.E. Taylor, London, Butterworths, 1963; 235-267. 6.Csaba, G.Y., Prigli, M., Bekesi, E., Kovacs -Gayer, E., Bajmocy, E. and Fazekas, B.: Septicemia in Silver Carp (Hypophtalmalmichthys molitrix, Val.) and Bighead (Aristichhthys nobilis, Rich) Caused by Pseudomonas fluorescens. Proceeding of an International seminar on Fish, Pathogens and Enviroment in European Polyculture. June 23-27,
  2. Szarvas, Hungary, 1981; 111-123. 7.Daly, J. G.: Fish Diseases and Disorders, Volume 3:Viral, Bacterial and Fungal Infections. Ed. P.T.K.Woo and D.W. Bruno, 1999; 584-586. 8.Diler, Ö., Kubilay, A.: Gökkuşağı Alabalıklarında Yüksek Mortaliteye Neden Olan Hemorajik Septisemi Etkeni Pseudomonas sp. Üzerinde Bir Çalışma (Poster). II. International Symposium on Aquatic Products September 21-23, 1996; İstanbul - Türkiye. 9. Frerichs, G.N., Roberts, R. J.: The Bacteriology of Teleost.Fish Pathology. Ed: Ronald J. Roberts, Bailliere Tindall, London, 1989; 300-301. 10. Holt J.G., Krieg N.R.: Gram-Negative Aerobic/Microaerophilic Rods and Cocci. In: Bergey's Manual of Determinative Bacteriology. Ninth edition, Ed. William R. Hensyl, Williams & Wilkins, Baltimore, 1994; 93-168. 11.Nedoluha, P.C. and Westoff, D.: Microbiology of striped bass grown in three aquaculture systems. Food Microbiology, 1997; Vol. 14 (3), 255-264. 12.Post, G.: Bacterial Diseases of Fish. In: Textbook of Fish Health. Ed. Dr. George Post, T.F.H. Publications, 1987; 44-47. 13.Roberts, R.J.: The pathophysiology and Systematic Pathology of Teleosts. In: Fish Pathology. Ed. by Roberts R.J. Bailliere Tindall, London, 1978; 56-63. 14. Sakai, ML, Atsuta, Kobayashi, ML: Pseudomonas fluorescens isolataed from the diseased rainbow trout Oncorhynchus mykiss. The Kitasato Archives of Experimental Medicine, 1989; 62 (4), 157-162.