Journals / İstanbul Tıp Dergisi / 2011 / Cilt: 12 - Sayı: 2

Effect of Lipemia on glucose oxidase method

Glukoz oksidaz yöntemine lipeminin etkisi

Pages
61–64
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In pools with 160 mg/dl glucose concentration, the variation rates were: 3.17, 2.97, 3.09, 3.09, and 4.06, respectively.Conclusion: Our results support the idea that use of exogenous and endogenous triglycerides can produce different interference rates in lipemia interference studies.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Many factors have been defined that affect clinical biochemistry results.
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Key Findings & Data:In this study, we planned to investigate the reported lipemia interference by adding exogenous lipid during glucose measurements/analysis using the Siemens Advia system.Methods: We used 5 different levels of serum pools obtained from patients with high triglyceride levels.
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Conclusion & Impact:In pools with 160 mg/dl glucose concentration, the variation rates were: 3.17, 2.97, 3.09, 3.09, and 4.06, respectively.Conclusion: Our results support the idea that use of exogenous and endogenous triglycerides can produce different interference rates in lipemia interference studies.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Many factors have been defined that affect clinical biochemistry results. Lipemia holds an important place among these factors. In this study, we planned to investigate the reported lipemia interference by adding exogenous lipid during glucose measurements/analysis using the Siemens Advia system.Methods: We used 5 different levels of serum pools obtained from patients with high triglyceride levels. Instead of adding exogenous lipid, we added glucose to obtain different glucose concentrations.Results: As compared with the reference method, the variation rates in pools with normal glucose concentration were: 2.28, 2.08, 2.11, 2.10, and 2.85, respectively. In pools with 160 mg/dl glucose concentration, the variation rates were: 3.17, 2.97, 3.09, 3.09, and 4.06, respectively.Conclusion: Our results support the idea that use of exogenous and endogenous triglycerides can produce different interference rates in lipemia interference studies.

Özet

Amaç: Klinik biyokimya analiz sonuçlarını etkileyen birçok faktör tanımlanmıştır. Lipemi de biyokimya sonuçlarını etkileyen bu faktörler arasında önemli bir yer tutmaktadır. Bu çalışmada, laboratuvarımızda kullandığımız Simens Advia sistemlerindeki glukoz ölçümlerinde eksojen lipit ilavesiyle bildirilen lipemi interferansını araştırmayı planladık.Gereç ve Yöntem: Çalışmamızda eksojen lipit ilavesi yerine, trigliserit (TG) düzeyi yüksek hastalardan oluşturduğumuz 5 farklı düzeydeki serum havuzunu kullandık ve farklı glukoz konsantrasyonları için dışardan glukoz ilave ettik.Bulgular: Normal glukoz düzeylerindeki havuzlarımızın referans yöntem (hekzokinaz yöntemi) ile karşılaştırıldığında pozitif % değişim oranları sırasıyla: 2,28; 2,08; 2,11; 2,10; 2,85 iken; glukoz ilavesiyle düzeyi 160 mg/dl’ye getirilen havuzlarda % değişim oranları: 3,17; 2,97; 3,09; 3,09; 4,06 olarak bulundu.Sonuç: Çalışma sonuçlarımız, lipemi interferansı çalışmalarında eksojen ve endojen TG kullanımının farklı interferans oranları bildirimine yol açabileceği görüşünü desteklemektedir.

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Cite This Article

ARAL, H., GÜVENEN, G., TOPKAYA, Ç., ÖZER, C., BERÇİK, B. İ., TONBAKLAR, P. B. (2022). Effect of Lipemia on glucose oxidase method. İstanbul Tıp Dergisi, Cilt: 12 - Sayı: 2, 61–64. https://ajindex.com/articles/552560

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